Melghirimyces profundicolus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Melghirimyces

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3716 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T6AW08|A0A2T6AW08_9BACL YD repeat-containing protein OS=Melghirimyces profundicolus OX=1242148 GN=C8P63_1484 PE=4 SV=1
MM1 pKa = 7.05NARR4 pKa = 11.84VAVEE8 pKa = 5.35DD9 pKa = 3.73GLQPVSQYY17 pKa = 11.42LQIQGCQVVKK27 pKa = 10.62LDD29 pKa = 3.91EE30 pKa = 5.15GSAQNCDD37 pKa = 3.38CCVISGGDD45 pKa = 3.5KK46 pKa = 11.41DD47 pKa = 3.88MMGMQDD53 pKa = 4.17TIGEE57 pKa = 4.26MQVINAEE64 pKa = 3.93GMTPEE69 pKa = 4.26EE70 pKa = 4.66VYY72 pKa = 10.69QAVQRR77 pKa = 11.84GMNQQNNQQNLQQ89 pKa = 3.42

Molecular weight: 9.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T6BQW0|A0A2T6BQW0_9BACL Uncharacterized membrane protein YcaP (DUF421 family) OS=Melghirimyces profundicolus OX=1242148 GN=C8P63_11667 PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 10.35RR3 pKa = 11.84SILSALMIGSAAWLFGRR20 pKa = 11.84SHH22 pKa = 7.51RR23 pKa = 11.84GQKK26 pKa = 9.54LLKK29 pKa = 10.03RR30 pKa = 11.84MMGRR34 pKa = 11.84RR35 pKa = 11.84PRR37 pKa = 11.84WMRR40 pKa = 11.84KK41 pKa = 6.42MGNMAFAGIASRR53 pKa = 11.84VFGRR57 pKa = 11.84NLMRR61 pKa = 11.84RR62 pKa = 11.84FGRR65 pKa = 3.7

Molecular weight: 7.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3716

0

3716

1052417

28

2189

283.2

31.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.9 ± 0.038

0.814 ± 0.012

5.06 ± 0.034

7.659 ± 0.056

3.916 ± 0.033

8.25 ± 0.034

2.285 ± 0.02

5.55 ± 0.036

5.233 ± 0.036

10.099 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.668 ± 0.022

2.995 ± 0.027

4.843 ± 0.029

3.25 ± 0.027

7.004 ± 0.053

5.392 ± 0.028

5.149 ± 0.029

7.641 ± 0.03

1.418 ± 0.018

2.874 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski