Listeria phage LWP01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3B6XGQ7|A0A3B6XGQ7_9CAUD Uncharacterized protein OS=Listeria phage LWP01 OX=1633782 GN=UE46_p05210 PE=4 SV=1
MM1 pKa = 7.45SGCEE5 pKa = 3.8YY6 pKa = 10.5CTDD9 pKa = 2.75NWEE12 pKa = 4.06ILTTFDD18 pKa = 4.19EE19 pKa = 5.45GPDD22 pKa = 3.47VEE24 pKa = 5.08GYY26 pKa = 9.89VAIRR30 pKa = 11.84DD31 pKa = 3.76DD32 pKa = 4.39SLVVSCDD39 pKa = 2.64WGMTAVKK46 pKa = 10.2MNFCPKK52 pKa = 10.11CGAALKK58 pKa = 10.42SRR60 pKa = 11.84NDD62 pKa = 3.4EE63 pKa = 4.32EE64 pKa = 4.61EE65 pKa = 4.58PEE67 pKa = 4.13LL68 pKa = 5.22

Molecular weight:
7.59 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3B6XH47|A0A3B6XH47_9CAUD HNHc domain-containing protein OS=Listeria phage LWP01 OX=1633782 GN=UE46_p05300 PE=4 SV=1
MM1 pKa = 7.64SDD3 pKa = 3.49VEE5 pKa = 4.53VVRR8 pKa = 11.84FFALLGFLLTVFAIFCYY25 pKa = 10.17KK26 pKa = 10.32VGYY29 pKa = 10.09HH30 pKa = 6.69DD31 pKa = 4.9ARR33 pKa = 11.84KK34 pKa = 9.8KK35 pKa = 9.99MKK37 pKa = 10.48GGGQIEE43 pKa = 4.74SIEE46 pKa = 4.28KK47 pKa = 10.07ARR49 pKa = 11.84HH50 pKa = 4.38EE51 pKa = 4.37AATSRR56 pKa = 11.84TSTHH60 pKa = 4.66KK61 pKa = 9.58TIPKK65 pKa = 9.91HH66 pKa = 4.82ILTRR70 pKa = 11.84NGVGSKK76 pKa = 10.28

Molecular weight:
8.48 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12824

67

1464

229.0

25.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.289 ± 0.487

0.671 ± 0.122

6.581 ± 0.267

8.32 ± 0.319

3.876 ± 0.28

6.535 ± 0.325

1.521 ± 0.177

6.098 ± 0.319

8.227 ± 0.432

8.718 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.667 ± 0.125

4.593 ± 0.301

2.846 ± 0.343

3.649 ± 0.247

4.609 ± 0.355

5.536 ± 0.268

5.895 ± 0.369

6.597 ± 0.324

1.146 ± 0.126

3.626 ± 0.261

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski