Gordonia phage William

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EGI8|A0A4Y6EGI8_9CAUD Serine integrase OS=Gordonia phage William OX=2571253 GN=40 PE=4 SV=1
MM1 pKa = 7.76IEE3 pKa = 4.29PSNLAHH9 pKa = 7.04FYY11 pKa = 10.78AAGEE15 pKa = 4.26GTYY18 pKa = 8.35WWHH21 pKa = 7.52DD22 pKa = 3.43AATDD26 pKa = 3.68AYY28 pKa = 10.77LITDD32 pKa = 4.1TFEE35 pKa = 4.53PPTDD39 pKa = 3.66DD40 pKa = 5.5ALYY43 pKa = 10.88LMPWDD48 pKa = 5.04GPWFAQWDD56 pKa = 4.41EE57 pKa = 4.04NWTAAAAQLNSVIARR72 pKa = 11.84HH73 pKa = 5.76EE74 pKa = 4.12EE75 pKa = 4.11AEE77 pKa = 4.25DD78 pKa = 3.36

Molecular weight: 8.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EEL8|A0A4Y6EEL8_9CAUD Uncharacterized protein OS=Gordonia phage William OX=2571253 GN=51 PE=4 SV=1
MM1 pKa = 7.47SLADD5 pKa = 4.07MDD7 pKa = 4.07QQNWDD12 pKa = 3.39AEE14 pKa = 4.11EE15 pKa = 4.24RR16 pKa = 11.84RR17 pKa = 11.84AQRR20 pKa = 11.84AARR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84TTPKK29 pKa = 9.33PATPADD35 pKa = 3.5VMPDD39 pKa = 3.43VEE41 pKa = 4.61RR42 pKa = 11.84DD43 pKa = 2.93RR44 pKa = 11.84HH45 pKa = 5.53LRR47 pKa = 11.84VASGRR52 pKa = 11.84PVFEE56 pKa = 5.08LADD59 pKa = 4.3LPPSGDD65 pKa = 2.98RR66 pKa = 11.84GIPEE70 pKa = 3.65VRR72 pKa = 11.84KK73 pKa = 10.28AFIAFCRR80 pKa = 11.84QHH82 pKa = 6.14PGRR85 pKa = 11.84WVRR88 pKa = 11.84YY89 pKa = 7.53NAAGIEE95 pKa = 4.24DD96 pKa = 3.96STATALRR103 pKa = 11.84HH104 pKa = 4.19QVRR107 pKa = 11.84RR108 pKa = 11.84RR109 pKa = 11.84TGGFTDD115 pKa = 3.98GFEE118 pKa = 4.16GALRR122 pKa = 11.84QLGSDD127 pKa = 3.42KK128 pKa = 10.43PSLYY132 pKa = 10.23VRR134 pKa = 11.84YY135 pKa = 10.18VSGGDD140 pKa = 3.14RR141 pKa = 11.84RR142 pKa = 3.71

Molecular weight: 16.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

16521

42

1872

199.0

21.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.094 ± 0.527

0.89 ± 0.166

7.221 ± 0.318

5.647 ± 0.239

2.724 ± 0.179

8.087 ± 0.376

1.949 ± 0.17

4.546 ± 0.18

3.347 ± 0.324

7.687 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.058 ± 0.169

2.778 ± 0.152

5.684 ± 0.283

3.426 ± 0.159

7.578 ± 0.437

5.417 ± 0.17

6.828 ± 0.377

7.802 ± 0.253

2.088 ± 0.122

2.149 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski