Fomitopsis pinicola (strain FP-58527) (Brown rot fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Fomitopsidaceae; Fomitopsis; Fomitopsis pinicola

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13806 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S8EF35|S8EF35_FOMPI Uncharacterized protein OS=Fomitopsis pinicola (strain FP-58527) OX=743788 GN=FOMPIDRAFT_1115832 PE=4 SV=1
MM1 pKa = 7.45HH2 pKa = 7.27SAHH5 pKa = 6.62NLLALVTLAVLASAAPSVTDD25 pKa = 3.19TAKK28 pKa = 10.74RR29 pKa = 11.84STCTVDD35 pKa = 3.41SVSSASSLSDD45 pKa = 3.2CSDD48 pKa = 3.44VVISSFTVDD57 pKa = 4.63DD58 pKa = 4.5GDD60 pKa = 4.0TVTMSFASGATVTMTGEE77 pKa = 4.05VTFASTTASGPLITFEE93 pKa = 4.51GDD95 pKa = 3.38SISFDD100 pKa = 3.26GGGNTFNGNGADD112 pKa = 3.58YY113 pKa = 10.59WDD115 pKa = 4.3GEE117 pKa = 4.44GTNGGVAKK125 pKa = 8.72PHH127 pKa = 6.79PFMKK131 pKa = 10.13IKK133 pKa = 10.83ASGTFEE139 pKa = 3.95NFIVLNTPAQAISVGNDD156 pKa = 3.14DD157 pKa = 5.23PLTISDD163 pKa = 3.65VTVNNLAGNTDD174 pKa = 3.8SLGHH178 pKa = 5.69NTDD181 pKa = 3.41GFDD184 pKa = 3.3VAASDD189 pKa = 3.88VTIKK193 pKa = 11.01NCVVEE198 pKa = 4.32NQDD201 pKa = 3.22DD202 pKa = 4.37CIAINSGSNIVFQDD216 pKa = 3.55NTCSGGHH223 pKa = 5.65GMSIGSIASGKK234 pKa = 7.22TVSGVIFSGNTVSDD248 pKa = 3.6SLYY251 pKa = 7.96GTRR254 pKa = 11.84VKK256 pKa = 10.62VDD258 pKa = 3.15SDD260 pKa = 3.61ATSGSVSNVTWSGNTISGISDD281 pKa = 3.53YY282 pKa = 11.36GVLITQSYY290 pKa = 8.96PDD292 pKa = 3.61NDD294 pKa = 3.98GTPGTDD300 pKa = 3.12TTISDD305 pKa = 4.38LNFIDD310 pKa = 3.7STTSVSVDD318 pKa = 3.02SGATRR323 pKa = 11.84VTVDD327 pKa = 3.64CGYY330 pKa = 8.94CTGTWDD336 pKa = 3.99WSQLDD341 pKa = 3.81ATGGDD346 pKa = 3.22AGTIVASDD354 pKa = 3.35ATIEE358 pKa = 4.34GGTYY362 pKa = 10.41

Molecular weight: 36.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S8FTB7|S8FTB7_FOMPI TPR_REGION domain-containing protein OS=Fomitopsis pinicola (strain FP-58527) OX=743788 GN=FOMPIDRAFT_1120590 PE=4 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84PSWSGRR8 pKa = 11.84RR9 pKa = 11.84PPPVHH14 pKa = 6.58RR15 pKa = 11.84HH16 pKa = 5.1AAPPPTQAVAPRR28 pKa = 11.84SPPRR32 pKa = 11.84PPPIAPAPALAARR45 pKa = 11.84ALTLAARR52 pKa = 11.84LRR54 pKa = 11.84PRR56 pKa = 11.84LLPP59 pKa = 4.12

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13805

1

13806

5596684

49

5042

405.4

44.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.521 ± 0.02

1.288 ± 0.01

5.712 ± 0.015

6.034 ± 0.021

3.459 ± 0.013

6.633 ± 0.019

2.617 ± 0.01

4.388 ± 0.015

4.168 ± 0.017

9.216 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.111 ± 0.008

3.034 ± 0.012

6.602 ± 0.027

3.676 ± 0.012

6.678 ± 0.021

8.051 ± 0.027

5.967 ± 0.015

6.633 ± 0.016

1.501 ± 0.006

2.71 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski