Pseudomonas phage MD8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0Z2L6|A0A1B0Z2L6_9CAUD Uncharacterized protein OS=Pseudomonas phage MD8 OX=1868596 PE=4 SV=1
MM1 pKa = 8.33DD2 pKa = 4.57DD3 pKa = 3.53TAEE6 pKa = 4.1PWALAIEE13 pKa = 4.58VDD15 pKa = 4.3CEE17 pKa = 4.03PLVLSEE23 pKa = 3.99MQEE26 pKa = 4.15YY27 pKa = 10.36AVTVTPPADD36 pKa = 3.42VLVVVAGDD44 pKa = 3.35QGPPGRR50 pKa = 11.84DD51 pKa = 3.34GVDD54 pKa = 3.22GAQWGATDD62 pKa = 3.15WW63 pKa = 3.73

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0Z001|A0A1B0Z001_9CAUD Lysis protein OS=Pseudomonas phage MD8 OX=1868596 PE=4 SV=1
MM1 pKa = 7.97ISIKK5 pKa = 10.58PEE7 pKa = 3.88GFQQQLADD15 pKa = 3.9LTEE18 pKa = 4.29LEE20 pKa = 4.3QRR22 pKa = 11.84QIPYY26 pKa = 8.86ATATALTRR34 pKa = 11.84TAQGLMDD41 pKa = 4.53RR42 pKa = 11.84LRR44 pKa = 11.84DD45 pKa = 3.56EE46 pKa = 4.08MRR48 pKa = 11.84VVFDD52 pKa = 4.2RR53 pKa = 11.84PTPYY57 pKa = 10.12TLNSLRR63 pKa = 11.84MVPARR68 pKa = 11.84KK69 pKa = 9.45DD70 pKa = 3.33RR71 pKa = 11.84LEE73 pKa = 3.87ARR75 pKa = 11.84VWFKK79 pKa = 11.63DD80 pKa = 3.61EE81 pKa = 4.8ADD83 pKa = 3.79GAQPASVWIAPEE95 pKa = 4.21VYY97 pKa = 10.21GGPRR101 pKa = 11.84RR102 pKa = 11.84NKK104 pKa = 9.51PAEE107 pKa = 3.87LQLRR111 pKa = 11.84AKK113 pKa = 10.65GILPEE118 pKa = 4.25GKK120 pKa = 9.97YY121 pKa = 9.77VVPGAGADD129 pKa = 3.49LDD131 pKa = 3.93RR132 pKa = 11.84YY133 pKa = 10.08GNIRR137 pKa = 11.84RR138 pKa = 11.84GQVTKK143 pKa = 10.52ALSGIRR149 pKa = 11.84GFSQAGYY156 pKa = 9.38NANATDD162 pKa = 3.7SRR164 pKa = 11.84RR165 pKa = 11.84SRR167 pKa = 11.84AKK169 pKa = 10.36GNARR173 pKa = 11.84RR174 pKa = 11.84YY175 pKa = 10.02FVMTRR180 pKa = 11.84KK181 pKa = 9.3GQPIGIAEE189 pKa = 4.02RR190 pKa = 11.84TGRR193 pKa = 11.84GRR195 pKa = 11.84DD196 pKa = 3.34AVSVIMAFVSRR207 pKa = 11.84PAYY210 pKa = 9.83RR211 pKa = 11.84RR212 pKa = 11.84RR213 pKa = 11.84LSFFEE218 pKa = 4.28IAQQYY223 pKa = 10.6ADD225 pKa = 3.82EE226 pKa = 4.28NLPRR230 pKa = 11.84EE231 pKa = 4.46FEE233 pKa = 4.03VAMRR237 pKa = 11.84GVAARR242 pKa = 11.84FAARR246 pKa = 11.84RR247 pKa = 3.66

Molecular weight: 27.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12975

52

827

202.7

22.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.576 ± 0.605

0.987 ± 0.14

5.588 ± 0.186

6.559 ± 0.295

2.805 ± 0.136

7.923 ± 0.318

1.873 ± 0.208

4.609 ± 0.193

3.931 ± 0.307

9.11 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.335 ± 0.144

3.114 ± 0.168

5.202 ± 0.266

4.247 ± 0.246

7.692 ± 0.385

6.335 ± 0.39

5.187 ± 0.278

6.659 ± 0.232

1.757 ± 0.191

2.513 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski