Amino acid dipepetide frequency for Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.761AlaAla: 2.761 ± 0.091
0.444AlaCys: 0.444 ± 0.033
2.494AlaAsp: 2.494 ± 0.062
4.115AlaGlu: 4.115 ± 0.087
2.867AlaPhe: 2.867 ± 0.066
4.681AlaGly: 4.681 ± 0.101
0.935AlaHis: 0.935 ± 0.032
5.055AlaIle: 5.055 ± 0.107
3.865AlaLys: 3.865 ± 0.08
7.656AlaLeu: 7.656 ± 0.12
1.923AlaMet: 1.923 ± 0.055
2.018AlaAsn: 2.018 ± 0.054
1.971AlaPro: 1.971 ± 0.063
1.531AlaGln: 1.531 ± 0.055
3.336AlaArg: 3.336 ± 0.071
4.695AlaSer: 4.695 ± 0.098
3.099AlaThr: 3.099 ± 0.072
5.422AlaVal: 5.422 ± 0.096
0.692AlaTrp: 0.692 ± 0.033
2.357AlaTyr: 2.357 ± 0.068
0.0AlaXaa: 0.0 ± 0.0
Cys
0.32CysAla: 0.32 ± 0.022
0.083CysCys: 0.083 ± 0.012
0.325CysAsp: 0.325 ± 0.028
0.414CysGlu: 0.414 ± 0.027
0.189CysPhe: 0.189 ± 0.018
0.698CysGly: 0.698 ± 0.042
0.134CysHis: 0.134 ± 0.013
0.336CysIle: 0.336 ± 0.024
0.315CysLys: 0.315 ± 0.022
0.425CysLeu: 0.425 ± 0.03
0.159CysMet: 0.159 ± 0.016
0.27CysAsn: 0.27 ± 0.02
0.536CysPro: 0.536 ± 0.033
0.128CysGln: 0.128 ± 0.016
0.353CysArg: 0.353 ± 0.023
0.461CysSer: 0.461 ± 0.027
0.292CysThr: 0.292 ± 0.024
0.483CysVal: 0.483 ± 0.026
0.056CysTrp: 0.056 ± 0.009
0.225CysTyr: 0.225 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
2.585AspAla: 2.585 ± 0.075
0.32AspCys: 0.32 ± 0.022
1.913AspAsp: 1.913 ± 0.061
3.662AspGlu: 3.662 ± 0.087
2.069AspPhe: 2.069 ± 0.053
2.999AspGly: 2.999 ± 0.067
0.731AspHis: 0.731 ± 0.036
3.395AspIle: 3.395 ± 0.076
2.666AspLys: 2.666 ± 0.073
5.41AspLeu: 5.41 ± 0.092
1.271AspMet: 1.271 ± 0.049
1.562AspAsn: 1.562 ± 0.052
2.791AspPro: 2.791 ± 0.063
1.276AspGln: 1.276 ± 0.046
2.583AspArg: 2.583 ± 0.069
2.808AspSer: 2.808 ± 0.067
1.834AspThr: 1.834 ± 0.053
4.592AspVal: 4.592 ± 0.096
0.573AspTrp: 0.573 ± 0.028
1.996AspTyr: 1.996 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
4.603GluAla: 4.603 ± 0.085
0.401GluCys: 0.401 ± 0.027
3.417GluAsp: 3.417 ± 0.08
6.067GluGlu: 6.067 ± 0.13
2.547GluPhe: 2.547 ± 0.064
4.698GluGly: 4.698 ± 0.099
0.793GluHis: 0.793 ± 0.038
6.08GluIle: 6.08 ± 0.115
4.945GluLys: 4.945 ± 0.102
7.136GluLeu: 7.136 ± 0.135
1.993GluMet: 1.993 ± 0.055
2.589GluAsn: 2.589 ± 0.063
2.041GluPro: 2.041 ± 0.053
1.457GluGln: 1.457 ± 0.045
4.507GluArg: 4.507 ± 0.102
3.651GluSer: 3.651 ± 0.087
2.956GluThr: 2.956 ± 0.076
6.345GluVal: 6.345 ± 0.092
0.658GluTrp: 0.658 ± 0.032
2.421GluTyr: 2.421 ± 0.066
0.0GluXaa: 0.0 ± 0.0
Phe
2.483PheAla: 2.483 ± 0.077
0.223PheCys: 0.223 ± 0.017
1.852PheAsp: 1.852 ± 0.059
2.002PheGlu: 2.002 ± 0.061
1.812PhePhe: 1.812 ± 0.074
2.874PheGly: 2.874 ± 0.071
0.809PheHis: 0.809 ± 0.035
2.642PheIle: 2.642 ± 0.074
2.16PheLys: 2.16 ± 0.059
4.882PheLeu: 4.882 ± 0.114
1.151PheMet: 1.151 ± 0.041
1.737PheAsn: 1.737 ± 0.058
2.013PhePro: 2.013 ± 0.058
1.149PheGln: 1.149 ± 0.036
2.179PheArg: 2.179 ± 0.061
3.69PheSer: 3.69 ± 0.078
2.486PheThr: 2.486 ± 0.064
3.277PheVal: 3.277 ± 0.069
0.461PheTrp: 0.461 ± 0.028
1.812PheTyr: 1.812 ± 0.058
0.0PheXaa: 0.0 ± 0.0
Gly
4.356GlyAla: 4.356 ± 0.105
0.49GlyCys: 0.49 ± 0.03
3.456GlyAsp: 3.456 ± 0.08
5.091GlyGlu: 5.091 ± 0.095
3.495GlyPhe: 3.495 ± 0.077
5.355GlyGly: 5.355 ± 0.121
1.129GlyHis: 1.129 ± 0.041
6.548GlyIle: 6.548 ± 0.105
5.789GlyLys: 5.789 ± 0.105
7.465GlyLeu: 7.465 ± 0.108
1.941GlyMet: 1.941 ± 0.066
3.002GlyAsn: 3.002 ± 0.087
2.149GlyPro: 2.149 ± 0.06
1.693GlyGln: 1.693 ± 0.058
3.619GlyArg: 3.619 ± 0.093
4.776GlySer: 4.776 ± 0.095
3.697GlyThr: 3.697 ± 0.104
6.519GlyVal: 6.519 ± 0.103
0.884GlyTrp: 0.884 ± 0.035
3.314GlyTyr: 3.314 ± 0.074
0.0GlyXaa: 0.0 ± 0.0
His
0.909HisAla: 0.909 ± 0.033
0.105HisCys: 0.105 ± 0.014
0.7HisAsp: 0.7 ± 0.033
1.068HisGlu: 1.068 ± 0.044
0.643HisPhe: 0.643 ± 0.032
1.338HisGly: 1.338 ± 0.047
0.336HisHis: 0.336 ± 0.023
0.784HisIle: 0.784 ± 0.035
0.55HisLys: 0.55 ± 0.029
1.445HisLeu: 1.445 ± 0.05
0.358HisMet: 0.358 ± 0.023
0.472HisAsn: 0.472 ± 0.026
0.853HisPro: 0.853 ± 0.039
0.297HisGln: 0.297 ± 0.022
0.757HisArg: 0.757 ± 0.037
0.9HisSer: 0.9 ± 0.038
0.592HisThr: 0.592 ± 0.03
1.417HisVal: 1.417 ± 0.049
0.153HisTrp: 0.153 ± 0.015
0.59HisTyr: 0.59 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.176IleAla: 5.176 ± 0.091
0.373IleCys: 0.373 ± 0.024
3.198IleAsp: 3.198 ± 0.085
4.139IleGlu: 4.139 ± 0.079
2.913IlePhe: 2.913 ± 0.07
4.929IleGly: 4.929 ± 0.09
1.093IleHis: 1.093 ± 0.04
4.606IleIle: 4.606 ± 0.109
3.865IleLys: 3.865 ± 0.087
7.818IleLeu: 7.818 ± 0.142
1.791IleMet: 1.791 ± 0.057
2.788IleAsn: 2.788 ± 0.061
4.126IlePro: 4.126 ± 0.087
1.765IleGln: 1.765 ± 0.048
3.922IleArg: 3.922 ± 0.085
6.294IleSer: 6.294 ± 0.101
4.123IleThr: 4.123 ± 0.076
5.832IleVal: 5.832 ± 0.084
0.583IleTrp: 0.583 ± 0.03
2.828IleTyr: 2.828 ± 0.07
0.0IleXaa: 0.0 ± 0.0
Lys
3.965LysAla: 3.965 ± 0.082
0.411LysCys: 0.411 ± 0.028
3.116LysAsp: 3.116 ± 0.074
5.327LysGlu: 5.327 ± 0.114
2.207LysPhe: 2.207 ± 0.058
4.748LysGly: 4.748 ± 0.088
0.64LysHis: 0.64 ± 0.036
4.682LysIle: 4.682 ± 0.087
3.406LysLys: 3.406 ± 0.085
6.397LysLeu: 6.397 ± 0.101
1.751LysMet: 1.751 ± 0.05
1.929LysAsn: 1.929 ± 0.055
2.271LysPro: 2.271 ± 0.058
1.167LysGln: 1.167 ± 0.048
3.792LysArg: 3.792 ± 0.084
3.233LysSer: 3.233 ± 0.07
2.789LysThr: 2.789 ± 0.068
5.864LysVal: 5.864 ± 0.101
0.656LysTrp: 0.656 ± 0.038
2.374LysTyr: 2.374 ± 0.062
0.0LysXaa: 0.0 ± 0.0
Leu
7.328LeuAla: 7.328 ± 0.124
0.494LeuCys: 0.494 ± 0.027
5.343LeuAsp: 5.343 ± 0.101
7.312LeuGlu: 7.312 ± 0.128
3.926LeuPhe: 3.926 ± 0.1
8.619LeuGly: 8.619 ± 0.147
1.512LeuHis: 1.512 ± 0.045
6.822LeuIle: 6.822 ± 0.129
6.417LeuLys: 6.417 ± 0.102
10.39LeuLeu: 10.39 ± 0.172
2.7LeuMet: 2.7 ± 0.068
4.435LeuAsn: 4.435 ± 0.09
4.414LeuPro: 4.414 ± 0.09
2.379LeuGln: 2.379 ± 0.068
6.13LeuArg: 6.13 ± 0.118
8.39LeuSer: 8.39 ± 0.128
5.451LeuThr: 5.451 ± 0.099
9.26LeuVal: 9.26 ± 0.137
0.832LeuTrp: 0.832 ± 0.041
3.58LeuTyr: 3.58 ± 0.071
0.0LeuXaa: 0.0 ± 0.0
Met
1.954MetAla: 1.954 ± 0.057
0.122MetCys: 0.122 ± 0.014
1.495MetAsp: 1.495 ± 0.045
2.129MetGlu: 2.129 ± 0.063
0.906MetPhe: 0.906 ± 0.038
2.26MetGly: 2.26 ± 0.063
0.265MetHis: 0.265 ± 0.022
2.046MetIle: 2.046 ± 0.056
2.16MetLys: 2.16 ± 0.057
2.044MetLeu: 2.044 ± 0.063
0.725MetMet: 0.725 ± 0.036
1.164MetAsn: 1.164 ± 0.037
0.982MetPro: 0.982 ± 0.04
0.467MetGln: 0.467 ± 0.029
1.766MetArg: 1.766 ± 0.057
1.871MetSer: 1.871 ± 0.048
1.335MetThr: 1.335 ± 0.043
1.873MetVal: 1.873 ± 0.059
0.275MetTrp: 0.275 ± 0.02
0.792MetTyr: 0.792 ± 0.038
0.0MetXaa: 0.0 ± 0.0
Asn
2.444AsnAla: 2.444 ± 0.067
0.298AsnCys: 0.298 ± 0.023
1.454AsnAsp: 1.454 ± 0.046
2.229AsnGlu: 2.229 ± 0.062
1.826AsnPhe: 1.826 ± 0.053
2.877AsnGly: 2.877 ± 0.097
0.429AsnHis: 0.429 ± 0.025
2.3AsnIle: 2.3 ± 0.057
1.838AsnLys: 1.838 ± 0.054
4.398AsnLeu: 4.398 ± 0.091
0.992AsnMet: 0.992 ± 0.042
1.354AsnAsn: 1.354 ± 0.054
2.419AsnPro: 2.419 ± 0.064
1.012AsnGln: 1.012 ± 0.041
1.813AsnArg: 1.813 ± 0.058
2.639AsnSer: 2.639 ± 0.074
1.879AsnThr: 1.879 ± 0.061
3.769AsnVal: 3.769 ± 0.085
0.461AsnTrp: 0.461 ± 0.024
1.69AsnTyr: 1.69 ± 0.055
0.0AsnXaa: 0.0 ± 0.0
Pro
2.243ProAla: 2.243 ± 0.054
0.251ProCys: 0.251 ± 0.021
2.054ProAsp: 2.054 ± 0.054
3.102ProGlu: 3.102 ± 0.068
2.169ProPhe: 2.169 ± 0.049
3.297ProGly: 3.297 ± 0.093
0.74ProHis: 0.74 ± 0.04
2.738ProIle: 2.738 ± 0.073
2.243ProLys: 2.243 ± 0.055
4.554ProLeu: 4.554 ± 0.09
1.053ProMet: 1.053 ± 0.042
1.573ProAsn: 1.573 ± 0.058
2.127ProPro: 2.127 ± 0.069
1.201ProGln: 1.201 ± 0.047
2.246ProArg: 2.246 ± 0.06
3.553ProSer: 3.553 ± 0.076
2.174ProThr: 2.174 ± 0.056
3.772ProVal: 3.772 ± 0.078
0.562ProTrp: 0.562 ± 0.029
1.943ProTyr: 1.943 ± 0.057
0.0ProXaa: 0.0 ± 0.0
Gln
1.591GlnAla: 1.591 ± 0.052
0.153GlnCys: 0.153 ± 0.017
1.32GlnAsp: 1.32 ± 0.047
2.03GlnGlu: 2.03 ± 0.062
0.926GlnPhe: 0.926 ± 0.033
2.288GlnGly: 2.288 ± 0.061
0.286GlnHis: 0.286 ± 0.019
1.641GlnIle: 1.641 ± 0.053
1.149GlnLys: 1.149 ± 0.043
2.371GlnLeu: 2.371 ± 0.066
0.57GlnMet: 0.57 ± 0.032
0.865GlnAsn: 0.865 ± 0.041
0.856GlnPro: 0.856 ± 0.043
0.611GlnGln: 0.611 ± 0.038
1.31GlnArg: 1.31 ± 0.047
1.251GlnSer: 1.251 ± 0.04
1.018GlnThr: 1.018 ± 0.047
2.36GlnVal: 2.36 ± 0.065
0.264GlnTrp: 0.264 ± 0.021
0.861GlnTyr: 0.861 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
3.356ArgAla: 3.356 ± 0.085
0.38ArgCys: 0.38 ± 0.026
3.277ArgAsp: 3.277 ± 0.083
5.31ArgGlu: 5.31 ± 0.106
2.135ArgPhe: 2.135 ± 0.07
4.246ArgGly: 4.246 ± 0.088
0.622ArgHis: 0.622 ± 0.026
4.176ArgIle: 4.176 ± 0.075
4.109ArgLys: 4.109 ± 0.084
5.141ArgLeu: 5.141 ± 0.098
1.326ArgMet: 1.326 ± 0.047
2.116ArgAsn: 2.116 ± 0.055
1.902ArgPro: 1.902 ± 0.063
1.01ArgGln: 1.01 ± 0.04
3.925ArgArg: 3.925 ± 0.094
3.114ArgSer: 3.114 ± 0.08
2.407ArgThr: 2.407 ± 0.061
4.87ArgVal: 4.87 ± 0.092
0.562ArgTrp: 0.562 ± 0.026
2.133ArgTyr: 2.133 ± 0.065
0.0ArgXaa: 0.0 ± 0.0
Ser
3.993SerAla: 3.993 ± 0.087
0.5SerCys: 0.5 ± 0.031
2.742SerAsp: 2.742 ± 0.064
3.715SerGlu: 3.715 ± 0.081
3.303SerPhe: 3.303 ± 0.075
5.029SerGly: 5.029 ± 0.089
1.095SerHis: 1.095 ± 0.041
4.752SerIle: 4.752 ± 0.09
3.995SerLys: 3.995 ± 0.08
8.754SerLeu: 8.754 ± 0.124
2.08SerMet: 2.08 ± 0.055
2.443SerAsn: 2.443 ± 0.081
3.542SerPro: 3.542 ± 0.081
2.124SerGln: 2.124 ± 0.067
4.104SerArg: 4.104 ± 0.092
6.013SerSer: 6.013 ± 0.125
3.868SerThr: 3.868 ± 0.106
5.344SerVal: 5.344 ± 0.088
0.864SerTrp: 0.864 ± 0.04
2.732SerTyr: 2.732 ± 0.071
0.0SerXaa: 0.0 ± 0.0
Thr
3.284ThrAla: 3.284 ± 0.071
0.322ThrCys: 0.322 ± 0.027
1.876ThrAsp: 1.876 ± 0.049
2.658ThrGlu: 2.658 ± 0.073
2.297ThrPhe: 2.297 ± 0.059
4.276ThrGly: 4.276 ± 0.082
0.728ThrHis: 0.728 ± 0.028
3.302ThrIle: 3.302 ± 0.082
2.458ThrLys: 2.458 ± 0.065
5.747ThrLeu: 5.747 ± 0.111
1.273ThrMet: 1.273 ± 0.05
1.696ThrAsn: 1.696 ± 0.062
2.758ThrPro: 2.758 ± 0.078
1.354ThrGln: 1.354 ± 0.048
2.678ThrArg: 2.678 ± 0.067
3.876ThrSer: 3.876 ± 0.091
2.864ThrThr: 2.864 ± 0.087
4.186ThrVal: 4.186 ± 0.096
0.611ThrTrp: 0.611 ± 0.03
2.062ThrTyr: 2.062 ± 0.066
0.0ThrXaa: 0.0 ± 0.0
Val
5.393ValAla: 5.393 ± 0.112
0.49ValCys: 0.49 ± 0.027
4.409ValAsp: 4.409 ± 0.082
6.103ValGlu: 6.103 ± 0.108
3.159ValPhe: 3.159 ± 0.075
5.908ValGly: 5.908 ± 0.094
1.198ValHis: 1.198 ± 0.044
7.14ValIle: 7.14 ± 0.113
6.384ValLys: 6.384 ± 0.114
8.134ValLeu: 8.134 ± 0.111
2.325ValMet: 2.325 ± 0.062
3.962ValAsn: 3.962 ± 0.084
3.489ValPro: 3.489 ± 0.071
1.86ValGln: 1.86 ± 0.058
4.513ValArg: 4.513 ± 0.093
6.1ValSer: 6.1 ± 0.114
4.851ValThr: 4.851 ± 0.095
7.407ValVal: 7.407 ± 0.112
0.67ValTrp: 0.67 ± 0.032
3.344ValTyr: 3.344 ± 0.081
0.0ValXaa: 0.0 ± 0.0
Trp
0.664TrpAla: 0.664 ± 0.031
0.064TrpCys: 0.064 ± 0.01
0.558TrpAsp: 0.558 ± 0.028
0.659TrpGlu: 0.659 ± 0.028
0.47TrpPhe: 0.47 ± 0.023
0.743TrpGly: 0.743 ± 0.034
0.151TrpHis: 0.151 ± 0.018
0.881TrpIle: 0.881 ± 0.042
0.732TrpLys: 0.732 ± 0.037
1.062TrpLeu: 1.062 ± 0.051
0.25TrpMet: 0.25 ± 0.018
0.576TrpAsn: 0.576 ± 0.033
0.297TrpPro: 0.297 ± 0.02
0.209TrpGln: 0.209 ± 0.019
0.633TrpArg: 0.633 ± 0.029
0.672TrpSer: 0.672 ± 0.032
0.49TrpThr: 0.49 ± 0.032
0.728TrpVal: 0.728 ± 0.035
0.166TrpTrp: 0.166 ± 0.017
0.448TrpTyr: 0.448 ± 0.031
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.508TyrAla: 2.508 ± 0.058
0.278TyrCys: 0.278 ± 0.023
2.038TyrAsp: 2.038 ± 0.061
2.172TyrGlu: 2.172 ± 0.059
1.834TyrPhe: 1.834 ± 0.054
2.906TyrGly: 2.906 ± 0.074
0.637TyrHis: 0.637 ± 0.031
2.233TyrIle: 2.233 ± 0.059
1.676TyrLys: 1.676 ± 0.059
4.504TyrLeu: 4.504 ± 0.085
0.996TyrMet: 0.996 ± 0.042
1.571TyrAsn: 1.571 ± 0.066
2.057TyrPro: 2.057 ± 0.061
1.034TyrGln: 1.034 ± 0.05
1.951TyrArg: 1.951 ± 0.057
3.006TyrSer: 3.006 ± 0.075
2.101TyrThr: 2.101 ± 0.071
3.525TyrVal: 3.525 ± 0.086
0.472TyrTrp: 0.472 ± 0.027
1.918TyrTyr: 1.918 ± 0.07
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2256 proteins (640304 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski