Clostridium sp. CAG:632

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2230 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5JZ48|R5JZ48_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:632 OX=1262830 GN=BN743_01264 PE=4 SV=1
MM1 pKa = 6.97TATGIEE7 pKa = 4.04HH8 pKa = 7.29DD9 pKa = 4.08AFMGTGVKK17 pKa = 10.3LLLEE21 pKa = 4.59NNTDD25 pKa = 3.36KK26 pKa = 11.46NVMVGCTALIVNNYY40 pKa = 8.09MISDD44 pKa = 4.26LFASEE49 pKa = 3.89VAAGKK54 pKa = 9.71KK55 pKa = 10.64ANDD58 pKa = 3.6TMYY61 pKa = 10.72MMSSDD66 pKa = 3.97LKK68 pKa = 11.0AAGISNIGQIEE79 pKa = 4.83IYY81 pKa = 10.18FHH83 pKa = 7.53VYY85 pKa = 10.74DD86 pKa = 4.27SDD88 pKa = 3.87TYY90 pKa = 11.59DD91 pKa = 3.66ALFDD95 pKa = 4.56SDD97 pKa = 4.3VVTIQTSKK105 pKa = 9.96YY106 pKa = 11.0NEE108 pKa = 3.96MDD110 pKa = 3.32LQADD114 pKa = 4.12EE115 pKa = 4.88EE116 pKa = 4.45EE117 pKa = 4.46SGYY120 pKa = 9.52EE121 pKa = 3.91LWNQDD126 pKa = 2.62GVRR129 pKa = 11.84IIGKK133 pKa = 9.39YY134 pKa = 8.8VDD136 pKa = 4.87EE137 pKa = 4.49NSFWGTGVLLYY148 pKa = 10.3IEE150 pKa = 4.41NTSGRR155 pKa = 11.84NIGINCDD162 pKa = 3.43DD163 pKa = 3.56MSINGFMVDD172 pKa = 3.74PVFSATVYY180 pKa = 10.52NEE182 pKa = 3.88KK183 pKa = 10.67KK184 pKa = 10.66SFDD187 pKa = 5.0DD188 pKa = 3.19ITIFSSDD195 pKa = 3.71LEE197 pKa = 4.33EE198 pKa = 5.07NGIDD202 pKa = 3.31KK203 pKa = 11.31VNDD206 pKa = 3.24IEE208 pKa = 5.01LSFHH212 pKa = 7.23IYY214 pKa = 10.58DD215 pKa = 4.02ADD217 pKa = 3.67SWSTICDD224 pKa = 3.04TGAITFSVDD233 pKa = 2.96QAAEE237 pKa = 3.78

Molecular weight: 26.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5KBB6|R5KBB6_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:632 OX=1262830 GN=BN743_01521 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.16KK9 pKa = 8.22KK10 pKa = 9.58SHH12 pKa = 5.81ARR14 pKa = 11.84VHH16 pKa = 6.18GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.89VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84KK40 pKa = 8.67HH41 pKa = 6.58LSAA44 pKa = 5.8

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2230

0

2230

701037

31

2242

314.4

35.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.511 ± 0.054

1.405 ± 0.023

5.648 ± 0.041

7.849 ± 0.049

3.728 ± 0.031

6.948 ± 0.051

1.742 ± 0.026

7.595 ± 0.043

6.246 ± 0.048

8.956 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.17 ± 0.026

4.343 ± 0.036

3.232 ± 0.032

3.787 ± 0.036

4.525 ± 0.033

5.512 ± 0.04

5.642 ± 0.052

6.951 ± 0.043

0.858 ± 0.019

4.349 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski