Streptomyces phage Mojorita

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Picardvirus; unclassified Picardvirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0MCD9|A0A1J0MCD9_9CAUD Uncharacterized protein OS=Streptomyces phage Mojorita OX=1920309 GN=SEA_MOJORITA_54 PE=4 SV=1
MM1 pKa = 6.94SRR3 pKa = 11.84VYY5 pKa = 10.05ATLDD9 pKa = 3.22QLAAWTGTPAPADD22 pKa = 3.69VEE24 pKa = 4.29RR25 pKa = 11.84LLARR29 pKa = 11.84ASSDD33 pKa = 2.63VDD35 pKa = 3.4AALMCAVYY43 pKa = 8.54DD44 pKa = 4.08TTADD48 pKa = 4.01GMPTDD53 pKa = 3.92PAVVQALADD62 pKa = 3.63ATCAQVEE69 pKa = 4.39YY70 pKa = 10.97QLATGDD76 pKa = 4.57DD77 pKa = 3.93GTGAAGRR84 pKa = 11.84WGSVSIGPVSLGDD97 pKa = 4.22RR98 pKa = 11.84RR99 pKa = 11.84DD100 pKa = 3.48APQAPGGLDD109 pKa = 3.4LAPRR113 pKa = 11.84AHH115 pKa = 7.13RR116 pKa = 11.84ALMQAGLLPGVICC129 pKa = 5.53

Molecular weight: 13.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0MCH9|A0A1J0MCH9_9CAUD Uncharacterized protein OS=Streptomyces phage Mojorita OX=1920309 GN=SEA_MOJORITA_2 PE=4 SV=1
MM1 pKa = 7.14TRR3 pKa = 11.84ATTARR8 pKa = 11.84LAAMRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84LDD17 pKa = 3.55EE18 pKa = 4.66PPPEE22 pKa = 4.12IVPGQLAVNVPAGTRR37 pKa = 11.84TPPKK41 pKa = 10.11ACEE44 pKa = 4.3HH45 pKa = 6.43GNPTCGARR53 pKa = 11.84PARR56 pKa = 11.84PYY58 pKa = 10.01PCGWKK63 pKa = 10.04CDD65 pKa = 3.53EE66 pKa = 4.5HH67 pKa = 7.95QPARR71 pKa = 11.84THH73 pKa = 7.19RR74 pKa = 11.84RR75 pKa = 11.84TTPP78 pKa = 2.81

Molecular weight: 8.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12289

43

780

219.4

23.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.73 ± 0.712

0.643 ± 0.106

6.746 ± 0.324

5.647 ± 0.3

1.741 ± 0.146

8.048 ± 0.516

2.148 ± 0.207

3.45 ± 0.224

1.912 ± 0.179

8.536 ± 0.415

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.668 ± 0.1

1.693 ± 0.16

6.998 ± 0.446

3.532 ± 0.187

9.317 ± 0.478

4.419 ± 0.266

7.031 ± 0.323

7.006 ± 0.265

1.815 ± 0.119

1.92 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski