Brochothrix campestris FSL F6-1037

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Listeriaceae; Brochothrix; Brochothrix campestris

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2423 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W7CZ26|W7CZ26_9LIST tRNA (guanine-N(1)-)-methyltransferase OS=Brochothrix campestris FSL F6-1037 OX=1265861 GN=trmD PE=3 SV=1
MM1 pKa = 7.12QLVLNEE7 pKa = 4.24SNEE10 pKa = 3.82IVAYY14 pKa = 10.37ALIGEE19 pKa = 4.32IEE21 pKa = 4.27GAVSYY26 pKa = 10.74EE27 pKa = 3.9LLLPRR32 pKa = 11.84QFDD35 pKa = 3.81RR36 pKa = 11.84LRR38 pKa = 11.84SDD40 pKa = 3.98DD41 pKa = 3.94GRR43 pKa = 11.84DD44 pKa = 3.35DD45 pKa = 3.84EE46 pKa = 4.85YY47 pKa = 11.57LYY49 pKa = 11.1GLPPNFEE56 pKa = 4.92ADD58 pKa = 4.28FKK60 pKa = 10.95PLYY63 pKa = 10.2FSLIDD68 pKa = 4.61DD69 pKa = 5.68RR70 pKa = 11.84IILNQNYY77 pKa = 9.25IEE79 pKa = 5.12PIEE82 pKa = 4.16EE83 pKa = 4.11QNNEE87 pKa = 4.03ALTLAEE93 pKa = 4.19LTEE96 pKa = 4.8MVAEE100 pKa = 4.69MYY102 pKa = 10.71LATNLNGGEE111 pKa = 3.99

Molecular weight: 12.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W7CY30|W7CY30_9LIST ABC transporter ATPase OS=Brochothrix campestris FSL F6-1037 OX=1265861 GN=BCAMP_01995 PE=4 SV=1
MM1 pKa = 7.14KK2 pKa = 9.31QTYY5 pKa = 7.54QPKK8 pKa = 9.49KK9 pKa = 7.65RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 9.98NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2423

0

2423

633698

22

1837

261.5

29.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.661 ± 0.066

0.7 ± 0.016

5.111 ± 0.051

6.346 ± 0.058

4.262 ± 0.04

6.449 ± 0.059

1.953 ± 0.023

7.427 ± 0.045

6.305 ± 0.055

9.844 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.711 ± 0.026

4.363 ± 0.036

3.419 ± 0.026

4.187 ± 0.044

4.032 ± 0.045

5.749 ± 0.037

6.73 ± 0.045

7.39 ± 0.048

0.878 ± 0.015

3.481 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski