Mycolicibacterium holsaticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5158 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3S0X4|A0A1E3S0X4_9MYCO GTPase Obg OS=Mycolicibacterium holsaticum OX=152142 GN=obg PE=3 SV=1
MM1 pKa = 7.21NRR3 pKa = 11.84PYY5 pKa = 9.9TAQSPYY11 pKa = 11.09SLTPTEE17 pKa = 4.33RR18 pKa = 11.84FGTYY22 pKa = 9.98AGEE25 pKa = 4.26PPVDD29 pKa = 3.62EE30 pKa = 5.66LDD32 pKa = 4.37YY33 pKa = 11.22PDD35 pKa = 5.86DD36 pKa = 4.3DD37 pKa = 4.54AGYY40 pKa = 10.05RR41 pKa = 11.84SSYY44 pKa = 9.09DD45 pKa = 2.96TGYY48 pKa = 9.03DD49 pKa = 2.79TGYY52 pKa = 11.05DD53 pKa = 3.34ADD55 pKa = 4.49YY56 pKa = 11.33DD57 pKa = 3.95DD58 pKa = 5.53TYY60 pKa = 11.86DD61 pKa = 3.76EE62 pKa = 4.75YY63 pKa = 11.79ADD65 pKa = 4.18EE66 pKa = 4.5YY67 pKa = 11.57ADD69 pKa = 5.02FEE71 pKa = 5.35DD72 pKa = 5.2DD73 pKa = 3.01EE74 pKa = 5.05ARR76 pKa = 11.84IDD78 pKa = 4.27RR79 pKa = 11.84RR80 pKa = 11.84WMWVAGIAGIILFIAVGTTGVILGGGDD107 pKa = 3.48SGSVTATGTSTAPSPSATATSAPRR131 pKa = 11.84ATAPAAPLVPSLPPEE146 pKa = 4.16TVTTVTPTAEE156 pKa = 4.29VTPTAQPVVPPAQAAPPPPAPSPRR180 pKa = 11.84TVTYY184 pKa = 10.07RR185 pKa = 11.84VTGNRR190 pKa = 11.84QLIDD194 pKa = 4.22LVTVIYY200 pKa = 10.54TDD202 pKa = 3.03AQGALRR208 pKa = 11.84TDD210 pKa = 3.67VNVALPWAKK219 pKa = 9.24TVVLDD224 pKa = 4.29PGVEE228 pKa = 4.03LSSVTATSVAGQLNCSITDD247 pKa = 3.3ASGALIAAQNNNTIITNCTKK267 pKa = 10.95

Molecular weight: 27.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3S0U2|A0A1E3S0U2_9MYCO TetR family transcriptional regulator OS=Mycolicibacterium holsaticum OX=152142 GN=BHQ17_05660 PE=4 SV=1
MM1 pKa = 7.61AHH3 pKa = 6.91RR4 pKa = 11.84KK5 pKa = 9.24KK6 pKa = 10.93GPSRR10 pKa = 11.84RR11 pKa = 11.84SGLSGVAVPRR21 pKa = 11.84AQARR25 pKa = 11.84ALHH28 pKa = 6.24SVDD31 pKa = 2.82IHH33 pKa = 6.64PPSSTDD39 pKa = 2.76GSFWGRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84VLLLNSTYY55 pKa = 10.82EE56 pKa = 4.11PLTALPMRR64 pKa = 11.84RR65 pKa = 11.84AVIMLMCGKK74 pKa = 10.39ADD76 pKa = 3.66VVHH79 pKa = 7.73DD80 pKa = 4.4DD81 pKa = 3.46PGGPVIHH88 pKa = 6.67SATRR92 pKa = 11.84TIVVPSVIRR101 pKa = 11.84LKK103 pKa = 10.81VYY105 pKa = 10.18VRR107 pKa = 11.84VPYY110 pKa = 10.07RR111 pKa = 11.84ARR113 pKa = 11.84VPMTRR118 pKa = 11.84AALMHH123 pKa = 6.99RR124 pKa = 11.84DD125 pKa = 3.56RR126 pKa = 11.84FRR128 pKa = 11.84CAYY131 pKa = 10.03CGGKK135 pKa = 10.0ADD137 pKa = 4.1TVDD140 pKa = 3.3HH141 pKa = 5.56VVPRR145 pKa = 11.84SRR147 pKa = 11.84GGEE150 pKa = 3.98HH151 pKa = 5.99SWEE154 pKa = 4.09NCVAACSTCNHH165 pKa = 6.31RR166 pKa = 11.84KK167 pKa = 9.52ADD169 pKa = 4.13RR170 pKa = 11.84LLGEE174 pKa = 4.82LGWTLRR180 pKa = 11.84STPLPPKK187 pKa = 8.18GQHH190 pKa = 4.74WRR192 pKa = 11.84LLSTVKK198 pKa = 10.49EE199 pKa = 4.39LDD201 pKa = 4.0PSWMRR206 pKa = 11.84YY207 pKa = 9.3LGEE210 pKa = 4.2GAAA213 pKa = 4.63

Molecular weight: 23.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5158

0

5158

1625613

30

3079

315.2

33.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.855 ± 0.045

0.84 ± 0.01

6.444 ± 0.032

5.335 ± 0.031

3.098 ± 0.019

8.715 ± 0.035

2.177 ± 0.017

4.253 ± 0.019

2.233 ± 0.02

9.691 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.126 ± 0.012

2.296 ± 0.018

5.843 ± 0.034

3.084 ± 0.02

7.237 ± 0.035

5.235 ± 0.018

6.056 ± 0.022

8.771 ± 0.035

1.509 ± 0.014

2.201 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski