Dragonfly associated cyclovirus 8

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Cyclovirus

Average proteome isoelectric point is 8.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M9V9N2|M9V9N2_9CIRC ATP-dependent helicase Rep OS=Dragonfly associated cyclovirus 8 OX=1574362 PE=3 SV=1
MM1 pKa = 7.54CNSTVRR7 pKa = 11.84KK8 pKa = 9.52FCFTWNNYY16 pKa = 7.32TEE18 pKa = 4.62HH19 pKa = 7.81DD20 pKa = 3.93EE21 pKa = 4.44NKK23 pKa = 10.2CKK25 pKa = 10.84DD26 pKa = 4.81FIAQYY31 pKa = 10.03CKK33 pKa = 10.59YY34 pKa = 10.45GIFGKK39 pKa = 10.35EE40 pKa = 3.71LAPTTNTPHH49 pKa = 6.25LQGYY53 pKa = 9.2CNLSKK58 pKa = 10.65PMRR61 pKa = 11.84FSTIKK66 pKa = 10.19KK67 pKa = 8.25HH68 pKa = 5.12LHH70 pKa = 5.16NSIHH74 pKa = 6.15IEE76 pKa = 3.86KK77 pKa = 10.82ANGSDD82 pKa = 3.63EE83 pKa = 4.12QNKK86 pKa = 9.6EE87 pKa = 4.02YY88 pKa = 10.39CSKK91 pKa = 10.72SGEE94 pKa = 4.12IFEE97 pKa = 5.06KK98 pKa = 9.94GTPIKK103 pKa = 10.38RR104 pKa = 11.84GQRR107 pKa = 11.84TDD109 pKa = 3.62LQSLLADD116 pKa = 3.37IQDD119 pKa = 3.91GNRR122 pKa = 11.84NIQTLAQSHH131 pKa = 5.76PTTYY135 pKa = 9.72IRR137 pKa = 11.84YY138 pKa = 8.25FRR140 pKa = 11.84GIHH143 pKa = 5.69TYY145 pKa = 11.14LNLVHH150 pKa = 7.2PIAPRR155 pKa = 11.84NFKK158 pKa = 10.11TDD160 pKa = 2.71TYY162 pKa = 10.15YY163 pKa = 11.14YY164 pKa = 9.18WGPPGSGKK172 pKa = 8.76SRR174 pKa = 11.84RR175 pKa = 11.84ALEE178 pKa = 4.02EE179 pKa = 3.36ATARR183 pKa = 11.84CNEE186 pKa = 4.13SIYY189 pKa = 10.53YY190 pKa = 9.62KK191 pKa = 10.54PRR193 pKa = 11.84GQWWDD198 pKa = 3.68GYY200 pKa = 9.12HH201 pKa = 4.78QQEE204 pKa = 4.6GVIIDD209 pKa = 5.22DD210 pKa = 4.3FYY212 pKa = 11.96GWIKK216 pKa = 10.71YY217 pKa = 10.27DD218 pKa = 3.78EE219 pKa = 4.42LLKK222 pKa = 10.49VTDD225 pKa = 4.36RR226 pKa = 11.84YY227 pKa = 9.86PYY229 pKa = 9.33KK230 pKa = 10.68VQVKK234 pKa = 10.33GSFEE238 pKa = 4.21EE239 pKa = 4.8FTSKK243 pKa = 10.73HH244 pKa = 4.18IWITSNVDD252 pKa = 2.75TCDD255 pKa = 3.11LYY257 pKa = 11.61KK258 pKa = 10.91FIGYY262 pKa = 7.93CTDD265 pKa = 4.06AIEE268 pKa = 5.0RR269 pKa = 11.84RR270 pKa = 11.84ITLKK274 pKa = 10.85SYY276 pKa = 10.13MSS278 pKa = 3.6

Molecular weight: 32.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M9V9N2|M9V9N2_9CIRC ATP-dependent helicase Rep OS=Dragonfly associated cyclovirus 8 OX=1574362 PE=3 SV=1
MM1 pKa = 7.27ARR3 pKa = 11.84YY4 pKa = 9.13RR5 pKa = 11.84RR6 pKa = 11.84LRR8 pKa = 11.84RR9 pKa = 11.84PLLRR13 pKa = 11.84RR14 pKa = 11.84NRR16 pKa = 11.84SSRR19 pKa = 11.84LRR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 8.47PLRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84FRR31 pKa = 11.84FKK33 pKa = 9.86RR34 pKa = 11.84TPVLYY39 pKa = 10.66VKK41 pKa = 9.37LTRR44 pKa = 11.84TVQVVSVINKK54 pKa = 9.88GGLNSLNQVLNDD66 pKa = 3.43FAEE69 pKa = 4.58FQNLAPSFEE78 pKa = 4.19RR79 pKa = 11.84VKK81 pKa = 10.79VYY83 pKa = 10.65RR84 pKa = 11.84LNVRR88 pKa = 11.84VFPHH92 pKa = 5.84QNVSNNSTSKK102 pKa = 9.83VPGYY106 pKa = 10.88AIVPYY111 pKa = 10.07HH112 pKa = 6.98RR113 pKa = 11.84PPPAATPSFSACLSIDD129 pKa = 3.19RR130 pKa = 11.84AKK132 pKa = 10.65VYY134 pKa = 10.52RR135 pKa = 11.84GTAFGRR141 pKa = 11.84MSLIPASRR149 pKa = 11.84LDD151 pKa = 3.52VEE153 pKa = 5.1ANTGEE158 pKa = 4.53KK159 pKa = 10.42YY160 pKa = 10.8SPDD163 pKa = 3.92RR164 pKa = 11.84IDD166 pKa = 3.21WKK168 pKa = 10.79PEE170 pKa = 3.65FEE172 pKa = 4.36ISSAAATQYY181 pKa = 11.02LYY183 pKa = 10.54TGFVIFEE190 pKa = 4.44TEE192 pKa = 3.58PTAPDD197 pKa = 3.97SEE199 pKa = 4.57TATYY203 pKa = 9.14TIVYY207 pKa = 9.6DD208 pKa = 3.79LYY210 pKa = 11.76VRR212 pKa = 11.84FKK214 pKa = 10.4NQRR217 pKa = 11.84SFII220 pKa = 4.23

Molecular weight: 25.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

498

220

278

249.0

29.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.221 ± 1.32

2.008 ± 0.999

4.418 ± 0.795

5.02 ± 0.598

5.02 ± 0.572

5.02 ± 1.183

2.41 ± 0.965

6.024 ± 1.244

6.627 ± 1.339

6.426 ± 0.837

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.004 ± 0.061

5.422 ± 0.021

5.422 ± 1.191

3.614 ± 0.571

8.835 ± 2.796

7.028 ± 0.742

7.229 ± 0.557

5.221 ± 2.197

1.406 ± 0.612

6.627 ± 0.754
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski