Gordonia phage Affeca

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vividuovirus; unclassified Vividuovirus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3M8G4|A0A3G3M8G4_9CAUD Uncharacterized protein OS=Gordonia phage Affeca OX=2483664 GN=4 PE=4 SV=1
MM1 pKa = 7.13SRR3 pKa = 11.84AVVYY7 pKa = 9.37AAWATTVVQIPDD19 pKa = 4.07DD20 pKa = 4.12VDD22 pKa = 3.09ATTPAEE28 pKa = 3.94IRR30 pKa = 11.84DD31 pKa = 3.57WADD34 pKa = 2.85EE35 pKa = 4.1HH36 pKa = 6.68FPGVSLCHH44 pKa = 5.29QCSHH48 pKa = 5.82EE49 pKa = 4.27VNLDD53 pKa = 3.07GDD55 pKa = 4.41FEE57 pKa = 7.1ADD59 pKa = 4.15TIVTDD64 pKa = 5.2DD65 pKa = 3.44GTEE68 pKa = 3.91YY69 pKa = 11.13DD70 pKa = 3.9VATGKK75 pKa = 9.4PRR77 pKa = 11.84YY78 pKa = 9.23EE79 pKa = 3.98PP80 pKa = 3.82

Molecular weight: 8.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3M8Q2|A0A3G3M8Q2_9CAUD Uncharacterized protein OS=Gordonia phage Affeca OX=2483664 GN=51 PE=4 SV=1
MM1 pKa = 6.86TAASFGRR8 pKa = 11.84VAWFGGDD15 pKa = 3.34LVEE18 pKa = 4.32VTGYY22 pKa = 8.92TGSGMVAVLTSHH34 pKa = 7.4RR35 pKa = 11.84SPAGEE40 pKa = 3.97VVVSPALASVADD52 pKa = 4.52VIVSAAPIPPRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84TVPTKK73 pKa = 10.88AGDD76 pKa = 3.43QFLLFDD82 pKa = 3.8TSGVV86 pKa = 3.51

Molecular weight: 8.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

18909

39

1677

217.3

23.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.052 ± 0.465

0.91 ± 0.152

7.531 ± 0.433

5.68 ± 0.341

2.554 ± 0.152

8.504 ± 0.442

2.29 ± 0.234

4.548 ± 0.253

2.517 ± 0.2

7.277 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.158 ± 0.099

2.644 ± 0.149

6.341 ± 0.268

3.713 ± 0.181

7.467 ± 0.404

4.797 ± 0.226

6.901 ± 0.26

7.853 ± 0.229

2.1 ± 0.119

2.163 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski