Microbacterium phage Johann

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Goodmanvirus; unclassified Goodmanvirus

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3M0C1|A0A3G3M0C1_9CAUD Uncharacterized protein OS=Microbacterium phage Johann OX=2484207 GN=33 PE=4 SV=1
MM1 pKa = 7.67SYY3 pKa = 9.42ATVATIRR10 pKa = 11.84RR11 pKa = 11.84YY12 pKa = 9.71YY13 pKa = 11.07DD14 pKa = 2.93LTNRR18 pKa = 11.84VAACVAVEE26 pKa = 4.04GLSIFPEE33 pKa = 4.03QWASEE38 pKa = 4.37KK39 pKa = 10.64AWALAAQPGWAEE51 pKa = 3.74AWEE54 pKa = 4.42SALVAHH60 pKa = 7.3KK61 pKa = 10.56DD62 pKa = 3.39DD63 pKa = 3.72EE64 pKa = 5.41AYY66 pKa = 10.98VPGLDD71 pKa = 3.99PGVITDD77 pKa = 4.01GMILSAVQALHH88 pKa = 6.9AAPQAEE94 pKa = 4.02EE95 pKa = 4.28SGVVEE100 pKa = 4.32SDD102 pKa = 3.47PEE104 pKa = 4.18EE105 pKa = 4.1VV106 pKa = 3.26

Molecular weight: 11.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3M0Q7|A0A3G3M0Q7_9CAUD DNA primase/polymerase OS=Microbacterium phage Johann OX=2484207 GN=50 PE=4 SV=1
MM1 pKa = 7.95PDD3 pKa = 2.93NRR5 pKa = 11.84PRR7 pKa = 11.84YY8 pKa = 9.48PEE10 pKa = 4.08EE11 pKa = 3.36TDD13 pKa = 3.17MNLTRR18 pKa = 11.84EE19 pKa = 4.23QRR21 pKa = 11.84RR22 pKa = 11.84HH23 pKa = 3.83VAKK26 pKa = 10.07WIYY29 pKa = 8.03RR30 pKa = 11.84ARR32 pKa = 11.84WILIAKK38 pKa = 8.25VVAVAKK44 pKa = 10.56LDD46 pKa = 4.15DD47 pKa = 3.98YY48 pKa = 11.36TPEE51 pKa = 3.89LEE53 pKa = 3.96RR54 pKa = 11.84RR55 pKa = 11.84KK56 pKa = 9.74NQEE59 pKa = 3.61LEE61 pKa = 3.87RR62 pKa = 11.84LQEE65 pKa = 4.07RR66 pKa = 11.84FEE68 pKa = 4.25GAIQSLPIWLRR79 pKa = 11.84SEE81 pKa = 4.32RR82 pKa = 11.84FLDD85 pKa = 3.45KK86 pKa = 10.93HH87 pKa = 5.67VVAPAEE93 pKa = 4.01RR94 pKa = 11.84HH95 pKa = 4.75FKK97 pKa = 10.55RR98 pKa = 11.84VGILQCARR106 pKa = 11.84EE107 pKa = 4.25IKK109 pKa = 10.39ALRR112 pKa = 11.84QGWMPP117 pKa = 4.03

Molecular weight: 14.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13581

39

978

219.0

23.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.023 ± 0.38

0.56 ± 0.12

6.384 ± 0.213

6.701 ± 0.362

2.746 ± 0.181

8.865 ± 0.382

1.745 ± 0.173

4.433 ± 0.348

4.617 ± 0.345

7.547 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.069 ± 0.109

3.196 ± 0.203

5.64 ± 0.357

4.072 ± 0.178

5.883 ± 0.382

5.309 ± 0.271

6.546 ± 0.313

7.4 ± 0.25

2.577 ± 0.252

2.688 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski