Roseobacter phage CRP-6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; unclassified Caudovirales

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A646QWK5|A0A646QWK5_9CAUD Putative tail structural protein OS=Roseobacter phage CRP-6 OX=2559285 GN=CRP6_gp46 PE=4 SV=1
MM1 pKa = 7.55ALVLKK6 pKa = 10.69DD7 pKa = 3.59RR8 pKa = 11.84VKK10 pKa = 10.36EE11 pKa = 4.29TTTTTGTGTYY21 pKa = 8.78TLAGAEE27 pKa = 4.68DD28 pKa = 4.41GFQAFSAIGDD38 pKa = 3.96GNITYY43 pKa = 7.8YY44 pKa = 10.45TATNAAGDD52 pKa = 3.91WEE54 pKa = 4.63VGIGTYY60 pKa = 7.76TASGTTLARR69 pKa = 11.84TTILSSSNGGSAVDD83 pKa = 3.31WSAGEE88 pKa = 3.91KK89 pKa = 9.91QVFVTQPASKK99 pKa = 10.5ASYY102 pKa = 8.46VDD104 pKa = 3.44EE105 pKa = 4.81NGFHH109 pKa = 7.12IGNIFEE115 pKa = 4.59KK116 pKa = 10.55CVEE119 pKa = 4.24LKK121 pKa = 8.41TTVANKK127 pKa = 8.99PAYY130 pKa = 9.42EE131 pKa = 4.1EE132 pKa = 4.45GRR134 pKa = 11.84LFYY137 pKa = 11.04DD138 pKa = 3.44KK139 pKa = 11.44AFGALAFYY147 pKa = 10.81NDD149 pKa = 3.92EE150 pKa = 4.42SDD152 pKa = 2.95ITLQIGQEE160 pKa = 4.1EE161 pKa = 5.19YY162 pKa = 10.46IRR164 pKa = 11.84VYY166 pKa = 11.17NDD168 pKa = 2.86TGATLLNGTPVYY180 pKa = 9.92LTGEE184 pKa = 4.22SGATPTVSAARR195 pKa = 11.84ADD197 pKa = 3.56TTYY200 pKa = 11.4AEE202 pKa = 4.29SQAVGVTTHH211 pKa = 7.36DD212 pKa = 3.77IEE214 pKa = 4.83NNTIGYY220 pKa = 6.12VTVRR224 pKa = 11.84GLIADD229 pKa = 3.75VDD231 pKa = 4.26TSHH234 pKa = 6.66LTVGEE239 pKa = 4.17PVHH242 pKa = 5.99VAVGGGTVTEE252 pKa = 4.55SPSYY256 pKa = 9.94PYY258 pKa = 10.78YY259 pKa = 7.51PTEE262 pKa = 3.95VGVCLISAAIGGCIYY277 pKa = 10.91VSISSEE283 pKa = 4.05TFQTMRR289 pKa = 11.84VDD291 pKa = 3.88GNSHH295 pKa = 6.63FDD297 pKa = 3.47ADD299 pKa = 3.87VTIDD303 pKa = 3.41GDD305 pKa = 4.14LVVNGTQTITSSNNIALSGAFSYY328 pKa = 10.82FNSGDD333 pKa = 3.63TIGADD338 pKa = 3.05NTSFTGTGTNDD349 pKa = 3.16GVFTGHH355 pKa = 6.57YY356 pKa = 10.13NGTSSNKK363 pKa = 8.16TYY365 pKa = 10.48YY366 pKa = 10.65VRR368 pKa = 11.84ISDD371 pKa = 4.16DD372 pKa = 3.02QSAPDD377 pKa = 3.52EE378 pKa = 4.79FEE380 pKa = 3.74WSLDD384 pKa = 3.4NFVTIEE390 pKa = 3.91ATGIAITTDD399 pKa = 3.39DD400 pKa = 3.46QALEE404 pKa = 3.96EE405 pKa = 4.23GVNIKK410 pKa = 10.34FNADD414 pKa = 3.24NGHH417 pKa = 5.73VLGDD421 pKa = 3.7VWSGTASPTNVDD433 pKa = 2.98TGIASNRR440 pKa = 11.84NTGTSGVGYY449 pKa = 6.79THH451 pKa = 6.88VGMYY455 pKa = 10.47YY456 pKa = 10.36DD457 pKa = 3.52VSSNYY462 pKa = 7.02WTFFDD467 pKa = 4.74EE468 pKa = 4.6YY469 pKa = 10.87APEE472 pKa = 4.08PTGTIDD478 pKa = 3.15TSHH481 pKa = 7.0ASFSYY486 pKa = 11.27GDD488 pKa = 3.27IKK490 pKa = 11.15VNSVIGDD497 pKa = 3.52VVGNLTGIASSATQLANNRR516 pKa = 11.84NITLSGDD523 pKa = 3.56VTGTAVFNGGADD535 pKa = 3.66ANITATVVNDD545 pKa = 3.29SHH547 pKa = 6.31THH549 pKa = 4.64DD550 pKa = 3.24TRR552 pKa = 11.84YY553 pKa = 10.15VQLAGDD559 pKa = 4.01TMSGTLNVPTVDD571 pKa = 3.99FGDD574 pKa = 3.55WTITEE579 pKa = 4.57SGGSLYY585 pKa = 10.46FAYY588 pKa = 10.4QGTNKK593 pKa = 10.24LKK595 pKa = 10.69LDD597 pKa = 3.63TSGTLSVTNDD607 pKa = 2.9VQTDD611 pKa = 3.47QTII614 pKa = 3.13

Molecular weight: 64.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A646QXS7|A0A646QXS7_9CAUD P4Hc domain-containing protein OS=Roseobacter phage CRP-6 OX=2559285 GN=CRP6_gp58 PE=4 SV=1
MM1 pKa = 8.5VDD3 pKa = 3.23MKK5 pKa = 11.35VNNSAYY11 pKa = 9.77KK12 pKa = 10.27KK13 pKa = 9.44LQSLEE18 pKa = 4.23EE19 pKa = 4.07YY20 pKa = 10.54AGQEE24 pKa = 4.57LEE26 pKa = 4.36DD27 pKa = 3.92KK28 pKa = 10.77LYY30 pKa = 11.0AIANDD35 pKa = 3.72AVRR38 pKa = 11.84TTLTSTSNKK47 pKa = 9.49GKK49 pKa = 10.26IGAVDD54 pKa = 3.44SGAYY58 pKa = 10.16LEE60 pKa = 4.75SFSFALGSGRR70 pKa = 11.84PRR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84VYY76 pKa = 10.56KK77 pKa = 10.64GSQKK81 pKa = 10.68SFNPEE86 pKa = 3.11QTALQNLLFDD96 pKa = 4.08IKK98 pKa = 10.81KK99 pKa = 9.57VDD101 pKa = 3.6LMSSTRR107 pKa = 11.84ITLRR111 pKa = 11.84NGSPYY116 pKa = 10.25ARR118 pKa = 11.84KK119 pKa = 9.53VEE121 pKa = 3.99YY122 pKa = 10.53NYY124 pKa = 10.76GYY126 pKa = 10.79RR127 pKa = 11.84IFAKK131 pKa = 10.23LRR133 pKa = 11.84RR134 pKa = 11.84KK135 pKa = 9.94HH136 pKa = 5.35GG137 pKa = 3.56

Molecular weight: 15.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

14172

44

895

205.4

22.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.355 ± 0.422

0.946 ± 0.137

6.739 ± 0.284

6.548 ± 0.334

3.662 ± 0.177

7.988 ± 0.657

1.672 ± 0.143

5.151 ± 0.212

6.097 ± 0.388

7.663 ± 0.365

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.745 ± 0.205

4.558 ± 0.237

3.458 ± 0.182

3.359 ± 0.193

4.283 ± 0.285

6.809 ± 0.321

7.472 ± 0.566

7.226 ± 0.238

1.63 ± 0.138

3.641 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski