Escherichia phage vB_EcoS_fFiEco02

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; unclassified Kagunavirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9VZ13|A0A7G9VZ13_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_fFiEco02 OX=2762426 GN=FE2_065 PE=4 SV=1
MM1 pKa = 5.98VQRR4 pKa = 11.84YY5 pKa = 7.43EE6 pKa = 3.99VEE8 pKa = 3.59KK9 pKa = 10.24WGLPEE14 pKa = 4.58PEE16 pKa = 4.15MAEE19 pKa = 4.14YY20 pKa = 10.08AYY22 pKa = 10.64GDD24 pKa = 3.8YY25 pKa = 11.22VEE27 pKa = 4.49YY28 pKa = 10.57DD29 pKa = 3.13AYY31 pKa = 11.37LKK33 pKa = 11.15LEE35 pKa = 4.16AKK37 pKa = 10.0LRR39 pKa = 11.84EE40 pKa = 4.11FAEE43 pKa = 3.91QVMYY47 pKa = 10.02EE48 pKa = 4.15PCSKK52 pKa = 9.44VTEE55 pKa = 4.17EE56 pKa = 4.05MARR59 pKa = 11.84EE60 pKa = 3.71ILGII64 pKa = 3.95

Molecular weight: 7.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9VZ07|A0A7G9VZ07_9CAUD DUF551 domain-containing protein OS=Escherichia phage vB_EcoS_fFiEco02 OX=2762426 GN=FE2_059 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84EE3 pKa = 3.91AFEE6 pKa = 3.82RR7 pKa = 11.84WAVVEE12 pKa = 3.95GLPVNKK18 pKa = 9.95GSKK21 pKa = 9.86KK22 pKa = 10.04EE23 pKa = 3.89YY24 pKa = 10.43LNVKK28 pKa = 8.31TRR30 pKa = 11.84LAWRR34 pKa = 11.84AWKK37 pKa = 10.42AGVLSVVNPRR47 pKa = 3.59

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

14060

29

824

182.6

20.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.043 ± 0.569

1.202 ± 0.158

6.074 ± 0.272

6.764 ± 0.381

3.777 ± 0.18

7.696 ± 0.243

1.679 ± 0.174

5.37 ± 0.143

5.953 ± 0.33

7.404 ± 0.253

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.461 ± 0.169

4.147 ± 0.228

3.713 ± 0.201

3.57 ± 0.406

5.505 ± 0.221

5.775 ± 0.303

6.444 ± 0.417

7.461 ± 0.332

1.422 ± 0.143

3.542 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski