Tremblaya princeps

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Tremblaya

Average proteome isoelectric point is 8.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 134 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143WMS1|A0A143WMS1_TREPR Homoserine dehydrogenase OS=Tremblaya princeps OX=189385 GN=thrA PE=4 SV=1
MM1 pKa = 7.91DD2 pKa = 5.07RR3 pKa = 11.84LEE5 pKa = 4.04SRR7 pKa = 11.84IMRR10 pKa = 11.84ILDD13 pKa = 3.36DD14 pKa = 4.88RR15 pKa = 11.84IGARR19 pKa = 11.84GGIGYY24 pKa = 10.18EE25 pKa = 4.08DD26 pKa = 3.74ALVRR30 pKa = 11.84HH31 pKa = 7.04GIDD34 pKa = 3.03SVDD37 pKa = 3.17IMEE40 pKa = 4.55SLVDD44 pKa = 3.5IEE46 pKa = 4.66CAFDD50 pKa = 3.92IEE52 pKa = 4.98FDD54 pKa = 4.35DD55 pKa = 5.64GMLTEE60 pKa = 5.26DD61 pKa = 3.82LSIRR65 pKa = 11.84DD66 pKa = 3.47VVDD69 pKa = 3.37ATRR72 pKa = 11.84RR73 pKa = 11.84LVHH76 pKa = 5.9VAMEE80 pKa = 4.53PKK82 pKa = 10.0VHH84 pKa = 6.6PP85 pKa = 4.42

Molecular weight: 9.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143WNJ9|A0A143WNJ9_TREPR 50S ribosomal protein L14 OS=Tremblaya princeps OX=189385 GN=rplN PE=3 SV=1
MM1 pKa = 7.71SMARR5 pKa = 11.84VGRR8 pKa = 11.84TRR10 pKa = 11.84GHH12 pKa = 6.3IRR14 pKa = 11.84LQLVRR19 pKa = 11.84SPITSPRR26 pKa = 11.84RR27 pKa = 11.84QRR29 pKa = 11.84LCVRR33 pKa = 11.84CLGLRR38 pKa = 11.84RR39 pKa = 11.84MWSIRR44 pKa = 11.84TLPLTPHH51 pKa = 6.45SLGAAARR58 pKa = 11.84VQHH61 pKa = 6.31LVRR64 pKa = 11.84ILL66 pKa = 3.33

Molecular weight: 7.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

134

0

134

33941

38

1343

253.3

27.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.46 ± 0.33

3.173 ± 0.151

4.178 ± 0.187

3.695 ± 0.189

1.927 ± 0.092

8.921 ± 0.176

3.415 ± 0.143

4.443 ± 0.149

2.207 ± 0.177

9.128 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.506 ± 0.099

2.071 ± 0.09

4.222 ± 0.12

2.507 ± 0.107

10.465 ± 0.198

7.46 ± 0.17

4.826 ± 0.116

8.2 ± 0.199

0.854 ± 0.073

2.342 ± 0.092
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski