Rubrobacter taiwanensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Rubrobacteria; Rubrobacterales; Rubrobacteraceae; Rubrobacter

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3014 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R1BS95|A0A4R1BS95_9ACTN PA0069 family radical SAM protein OS=Rubrobacter taiwanensis OX=185139 GN=E0L93_00075 PE=4 SV=1
MM1 pKa = 7.33LRR3 pKa = 11.84VAHH6 pKa = 6.79LSPDD10 pKa = 3.38APNVDD15 pKa = 3.36VYY17 pKa = 11.8LNDD20 pKa = 3.41EE21 pKa = 4.56PVAEE25 pKa = 4.38LQGVPYY31 pKa = 9.08GTISPYY37 pKa = 10.97LPVPAGTQNVKK48 pKa = 10.25VYY50 pKa = 10.72AAGDD54 pKa = 3.39TSEE57 pKa = 4.44PVIEE61 pKa = 4.97ADD63 pKa = 3.09VDD65 pKa = 4.05LAAGADD71 pKa = 3.81YY72 pKa = 10.45TVGAVGLVEE81 pKa = 5.52DD82 pKa = 5.24GSLTAQVYY90 pKa = 10.3QDD92 pKa = 5.29DD93 pKa = 4.28NTPPAGDD100 pKa = 3.51NARR103 pKa = 11.84LRR105 pKa = 11.84VVHH108 pKa = 6.8ASPDD112 pKa = 3.5AGPVDD117 pKa = 4.05VGAQGGGNLVEE128 pKa = 4.93GLEE131 pKa = 4.36FPNASDD137 pKa = 3.84YY138 pKa = 12.02AEE140 pKa = 4.6VPAGTYY146 pKa = 9.24TIEE149 pKa = 4.03VKK151 pKa = 10.68AAGTDD156 pKa = 3.79QVALTVPDD164 pKa = 3.5VALNAGVVYY173 pKa = 10.67SAFAVGLAEE182 pKa = 5.85DD183 pKa = 4.53GSLDD187 pKa = 4.09VILTEE192 pKa = 5.92DD193 pKa = 3.7SMAMPDD199 pKa = 3.47TGGLSPGGVLLAGSLLFFSAAAAGILLLRR228 pKa = 11.84RR229 pKa = 11.84RR230 pKa = 11.84LTT232 pKa = 3.4

Molecular weight: 23.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R1BFP3|A0A4R1BFP3_9ACTN Shikimate dehydrogenase OS=Rubrobacter taiwanensis OX=185139 GN=E0L93_11830 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.21QPNRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.57RR12 pKa = 11.84HH13 pKa = 4.5KK14 pKa = 7.95THH16 pKa = 6.0GFRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84TTAGRR28 pKa = 11.84KK29 pKa = 8.66ILAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.04GRR39 pKa = 11.84KK40 pKa = 8.79RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3014

0

3014

923775

28

1613

306.5

33.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.305 ± 0.055

0.754 ± 0.013

4.597 ± 0.034

8.446 ± 0.061

3.518 ± 0.029

9.559 ± 0.045

1.802 ± 0.018

4.496 ± 0.038

2.268 ± 0.027

10.765 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.04 ± 0.017

2.073 ± 0.019

5.366 ± 0.03

2.307 ± 0.028

8.988 ± 0.055

5.021 ± 0.026

4.421 ± 0.028

8.369 ± 0.041

1.228 ± 0.021

2.676 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski