Bacillus phage vB_BtS_B83

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skryabinvirinae; Pushchinovirus; Bacillus virus B83

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8MV16|A0A4P8MV16_9CAUD Uncharacterized protein OS=Bacillus phage vB_BtS_B83 OX=2565501 GN=B83_gp71 PE=4 SV=1
MM1 pKa = 7.61EE2 pKa = 5.84EE3 pKa = 3.67ILNIEE8 pKa = 3.96EE9 pKa = 4.22HH10 pKa = 5.15QEE12 pKa = 3.95KK13 pKa = 9.83VQWSSMSGYY22 pKa = 10.84AITTNEE28 pKa = 3.87QVIKK32 pKa = 10.79LLIDD36 pKa = 4.51DD37 pKa = 4.71EE38 pKa = 4.68QSCCEE43 pKa = 3.67NFGYY47 pKa = 10.5FMSEE51 pKa = 3.69DD52 pKa = 3.98DD53 pKa = 4.4FNDD56 pKa = 4.71FIGAEE61 pKa = 4.26LIDD64 pKa = 3.97VKK66 pKa = 10.58ITDD69 pKa = 3.85TEE71 pKa = 4.26LKK73 pKa = 10.36EE74 pKa = 4.27GLLEE78 pKa = 4.29KK79 pKa = 10.37HH80 pKa = 6.8DD81 pKa = 4.75LDD83 pKa = 4.69IEE85 pKa = 4.34SEE87 pKa = 4.33YY88 pKa = 11.34FEE90 pKa = 5.97GDD92 pKa = 3.61VMFVDD97 pKa = 4.26IVTSKK102 pKa = 9.89GTLQFVAYY110 pKa = 9.56NEE112 pKa = 4.1HH113 pKa = 6.07NGYY116 pKa = 9.63YY117 pKa = 9.8GHH119 pKa = 6.2EE120 pKa = 4.13AKK122 pKa = 10.79VISKK126 pKa = 9.73QINHH130 pKa = 7.05DD131 pKa = 3.68EE132 pKa = 4.22VLL134 pKa = 3.63

Molecular weight: 15.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8MXQ2|A0A4P8MXQ2_9CAUD Putative tail assembly chaperone OS=Bacillus phage vB_BtS_B83 OX=2565501 GN=B83_gp18 PE=4 SV=1
MM1 pKa = 8.1RR2 pKa = 11.84EE3 pKa = 4.22LLRR6 pKa = 11.84IWIVQVRR13 pKa = 11.84WANKK17 pKa = 8.44YY18 pKa = 9.09YY19 pKa = 10.83RR20 pKa = 11.84NGLPLYY26 pKa = 9.1FLHH29 pKa = 6.93KK30 pKa = 10.42FGIIKK35 pKa = 10.37DD36 pKa = 3.64PSLWLSVYY44 pKa = 7.42TFKK47 pKa = 11.22EE48 pKa = 4.59DD49 pKa = 3.52IRR51 pKa = 11.84FWWKK55 pKa = 9.21VRR57 pKa = 11.84KK58 pKa = 9.73GIPKK62 pKa = 10.04

Molecular weight: 7.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

14755

41

1720

207.8

23.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.838 ± 0.498

0.928 ± 0.168

6.012 ± 0.205

8.526 ± 0.341

3.66 ± 0.158

6.086 ± 0.515

1.464 ± 0.129

7.408 ± 0.273

9.048 ± 0.267

7.862 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.16

5.449 ± 0.241

2.813 ± 0.26

3.985 ± 0.246

4.541 ± 0.288

5.693 ± 0.252

5.74 ± 0.44

6.459 ± 0.238

1.294 ± 0.151

3.497 ± 0.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski