Acidiphilium cryptum (strain JF-5)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Acidiphilium; Acidiphilium cryptum

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3521 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5FXZ4|A5FXZ4_ACICJ Uncharacterized protein OS=Acidiphilium cryptum (strain JF-5) OX=349163 GN=Acry_1265 PE=4 SV=1
MM1 pKa = 7.93DD2 pKa = 6.3RR3 pKa = 11.84IAPFAPALILAALGIAVLGLAGCTTTAGAPYY34 pKa = 10.17GGSAYY39 pKa = 10.06YY40 pKa = 10.48APPSFGYY47 pKa = 9.58GAPYY51 pKa = 10.29GYY53 pKa = 10.65GAGYY57 pKa = 9.69GPAYY61 pKa = 9.94GPIYY65 pKa = 9.84QYY67 pKa = 11.02PDD69 pKa = 3.38YY70 pKa = 11.42APDD73 pKa = 4.94DD74 pKa = 3.83EE75 pKa = 5.82HH76 pKa = 9.52ADD78 pKa = 3.7DD79 pKa = 6.39DD80 pKa = 5.75DD81 pKa = 6.22RR82 pKa = 11.84FDD84 pKa = 6.42DD85 pKa = 5.4DD86 pKa = 5.44DD87 pKa = 6.16HH88 pKa = 8.98DD89 pKa = 4.49GDD91 pKa = 4.65RR92 pKa = 11.84AGDD95 pKa = 3.58HH96 pKa = 7.4DD97 pKa = 5.09GDD99 pKa = 5.15HH100 pKa = 7.71DD101 pKa = 5.33DD102 pKa = 5.23DD103 pKa = 6.07HH104 pKa = 7.6DD105 pKa = 3.82GHH107 pKa = 7.25

Molecular weight: 11.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5FWE9|A5FWE9_ACICJ Potassium-transporting ATPase KdpC subunit OS=Acidiphilium cryptum (strain JF-5) OX=349163 GN=kdpC PE=3 SV=1
MM1 pKa = 7.36SKK3 pKa = 10.58KK4 pKa = 10.06PGAVPKK10 pKa = 10.3SGRR13 pKa = 11.84RR14 pKa = 11.84LIRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84NLLAHH24 pKa = 6.98PRR26 pKa = 11.84PVMLVSGNDD35 pKa = 3.06GRR37 pKa = 11.84RR38 pKa = 11.84PRR40 pKa = 11.84IVRR43 pKa = 11.84LPAPLRR49 pKa = 11.84RR50 pKa = 11.84LHH52 pKa = 6.72RR53 pKa = 11.84RR54 pKa = 11.84ALRR57 pKa = 11.84LRR59 pKa = 11.84RR60 pKa = 11.84GGGRR64 pKa = 11.84APPARR69 pKa = 11.84QPHH72 pKa = 6.52HH73 pKa = 7.03PRR75 pKa = 11.84LDD77 pKa = 3.69PPAPPLRR84 pKa = 11.84PPRR87 pKa = 11.84SPCHH91 pKa = 5.03QAASPGAPPSRR102 pKa = 11.84PSRR105 pKa = 11.84PSRR108 pKa = 11.84PLRR111 pKa = 11.84PAARR115 pKa = 11.84SPARR119 pKa = 11.84PWLAPPRR126 pKa = 11.84LSRR129 pKa = 11.84HH130 pKa = 6.31RR131 pKa = 11.84IPRR134 pKa = 11.84PATPRR139 pKa = 11.84PARR142 pKa = 11.84CAGHH146 pKa = 6.86AGLHH150 pKa = 5.51RR151 pKa = 11.84RR152 pKa = 11.84HH153 pKa = 6.41PRR155 pKa = 11.84RR156 pKa = 11.84RR157 pKa = 11.84PHH159 pKa = 6.77PPPALPHH166 pKa = 5.97HH167 pKa = 6.23RR168 pKa = 11.84HH169 pKa = 5.74RR170 pKa = 11.84PAARR174 pKa = 11.84PRR176 pKa = 11.84AAAEE180 pKa = 3.81TAPTPRR186 pKa = 11.84RR187 pKa = 11.84TNGRR191 pKa = 11.84NARR194 pKa = 11.84PAARR198 pKa = 11.84SGTRR202 pKa = 11.84PGTRR206 pKa = 11.84PDD208 pKa = 3.65APSAAARR215 pKa = 11.84TPRR218 pKa = 11.84RR219 pKa = 11.84AARR222 pKa = 11.84RR223 pKa = 11.84RR224 pKa = 11.84LAPPARR230 pKa = 11.84SPAPNPLLSPVLTSRR245 pKa = 11.84RR246 pKa = 11.84ARR248 pKa = 11.84APGPRR253 pKa = 11.84PMRR256 pKa = 11.84LTRR259 pKa = 11.84SPADD263 pKa = 3.47PKK265 pKa = 11.03RR266 pKa = 11.84NSS268 pKa = 3.0

Molecular weight: 29.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3521

0

3521

1166442

39

2887

331.3

35.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.728 ± 0.072

0.864 ± 0.012

5.249 ± 0.031

5.072 ± 0.044

3.497 ± 0.028

9.054 ± 0.048

2.262 ± 0.019

5.119 ± 0.03

2.338 ± 0.033

10.419 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.343 ± 0.018

2.283 ± 0.029

5.72 ± 0.034

2.724 ± 0.022

8.159 ± 0.051

4.732 ± 0.037

5.14 ± 0.036

7.035 ± 0.033

1.291 ± 0.015

1.972 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski