Streptococcus phage 31B4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P0VI16|A0A2P0VI16_9CAUD Receptor binding protein OS=Streptococcus phage 31B4 OX=2041495 GN=31B4_017 PE=4 SV=1
MM1 pKa = 7.3SVSKK5 pKa = 9.08EE6 pKa = 4.26TIMQTLNLDD15 pKa = 3.8EE16 pKa = 4.97TDD18 pKa = 3.78DD19 pKa = 3.8TALIPAYY26 pKa = 9.65IEE28 pKa = 3.9SARR31 pKa = 11.84QYY33 pKa = 10.3VVNSVGDD40 pKa = 3.54DD41 pKa = 3.37PKK43 pKa = 10.95FYY45 pKa = 11.07NLDD48 pKa = 3.41SVRR51 pKa = 11.84ALFDD55 pKa = 3.34TAVIALTSSYY65 pKa = 8.66FTYY68 pKa = 10.07RR69 pKa = 11.84VALTDD74 pKa = 3.37TATYY78 pKa = 9.41PVNLTLNSIIGQLRR92 pKa = 11.84GLYY95 pKa = 8.66ATYY98 pKa = 10.56SEE100 pKa = 4.46EE101 pKa = 5.06RR102 pKa = 11.84GDD104 pKa = 3.53

Molecular weight: 11.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P0VG79|A0A2P0VG79_9CAUD Terminase OS=Streptococcus phage 31B4 OX=2041495 GN=31B4_001 PE=4 SV=1
MM1 pKa = 7.56PMVRR5 pKa = 11.84RR6 pKa = 11.84CKK8 pKa = 10.63ADD10 pKa = 2.94GCRR13 pKa = 11.84SLTEE17 pKa = 4.33RR18 pKa = 11.84PAHH21 pKa = 5.85YY22 pKa = 7.72CTTHH26 pKa = 7.43KK27 pKa = 10.96SMEE30 pKa = 3.82AAYY33 pKa = 7.91TQEE36 pKa = 3.76RR37 pKa = 11.84QRR39 pKa = 11.84YY40 pKa = 8.48SRR42 pKa = 11.84TRR44 pKa = 11.84YY45 pKa = 8.96NKK47 pKa = 9.75RR48 pKa = 11.84VRR50 pKa = 11.84NRR52 pKa = 11.84DD53 pKa = 3.33DD54 pKa = 3.39EE55 pKa = 4.57TKK57 pKa = 10.41EE58 pKa = 3.75RR59 pKa = 11.84YY60 pKa = 9.28AFYY63 pKa = 10.69RR64 pKa = 11.84SRR66 pKa = 11.84TWSSIRR72 pKa = 11.84KK73 pKa = 8.59IALEE77 pKa = 4.04RR78 pKa = 11.84DD79 pKa = 3.31NYY81 pKa = 10.11LCQYY85 pKa = 10.34CLALDD90 pKa = 3.82VVTPDD95 pKa = 3.53SRR97 pKa = 11.84IGDD100 pKa = 3.58HH101 pKa = 5.32VTPVEE106 pKa = 3.97IAPEE110 pKa = 4.07LKK112 pKa = 9.69TDD114 pKa = 3.36VSNIVATCRR123 pKa = 11.84SCDD126 pKa = 3.27NTKK129 pKa = 9.28RR130 pKa = 11.84TLEE133 pKa = 3.83QEE135 pKa = 4.04IYY137 pKa = 9.73GTGQNRR143 pKa = 11.84TKK145 pKa = 10.92QNTDD149 pKa = 2.61LRR151 pKa = 11.84LSVAAWAGLIARR163 pKa = 11.84KK164 pKa = 9.53KK165 pKa = 9.95ADD167 pKa = 3.35VVKK170 pKa = 10.34PLL172 pKa = 3.7

Molecular weight: 19.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

40

0

40

11196

47

1656

279.9

31.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.431 ± 0.433

0.447 ± 0.115

6.663 ± 0.268

6.61 ± 0.516

4.466 ± 0.24

6.824 ± 0.562

1.465 ± 0.126

6.654 ± 0.22

8.244 ± 0.491

7.797 ± 0.424

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.474 ± 0.161

6.565 ± 0.281

3.278 ± 0.188

4.001 ± 0.275

4.171 ± 0.259

6.27 ± 0.263

6.199 ± 0.381

6.083 ± 0.321

1.545 ± 0.16

3.805 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski