Escherichia phage PHB10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481W3S6|A0A481W3S6_9CAUD Uncharacterized protein OS=Escherichia phage PHB10 OX=2530035 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 10.37EE3 pKa = 3.39GFYY6 pKa = 10.03WIQHH10 pKa = 5.03NGRR13 pKa = 11.84VQVAYY18 pKa = 10.05YY19 pKa = 10.68SNGVTEE25 pKa = 4.75DD26 pKa = 4.32LEE28 pKa = 4.46TGLIFNGIWHH38 pKa = 7.01LTQGDD43 pKa = 4.53DD44 pKa = 3.58ICDD47 pKa = 3.38NGEE50 pKa = 3.95AEE52 pKa = 4.69VIEE55 pKa = 5.08GPLPLPFRR63 pKa = 4.67

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481W3R2|A0A481W3R2_9CAUD Uncharacterized protein OS=Escherichia phage PHB10 OX=2530035 PE=4 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84LINRR6 pKa = 11.84GSKK9 pKa = 9.91QSPLARR15 pKa = 11.84QACEE19 pKa = 3.29IALAAHH25 pKa = 4.85QQRR28 pKa = 11.84YY29 pKa = 8.81GDD31 pKa = 3.75YY32 pKa = 10.25GRR34 pKa = 11.84SKK36 pKa = 10.11MKK38 pKa = 8.75EE39 pKa = 4.04TYY41 pKa = 7.6TVRR44 pKa = 11.84VDD46 pKa = 3.86GVKK49 pKa = 10.26VWVEE53 pKa = 3.75VVNCKK58 pKa = 10.32ASYY61 pKa = 10.3VATAMTGMRR70 pKa = 11.84RR71 pKa = 11.84LRR73 pKa = 11.84SLPGLANN80 pKa = 3.51

Molecular weight: 8.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

16582

37

1149

218.2

24.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.679 ± 0.316

1.176 ± 0.153

5.91 ± 0.207

6.037 ± 0.351

3.594 ± 0.167

7.478 ± 0.361

1.592 ± 0.166

5.729 ± 0.177

5.204 ± 0.265

8.636 ± 0.33

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.418 ± 0.104

4.547 ± 0.193

4.004 ± 0.231

4.191 ± 0.33

5.693 ± 0.271

6.573 ± 0.229

6.206 ± 0.284

6.513 ± 0.221

1.55 ± 0.093

3.269 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski