Monosporascus sp. CRB-9-2

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Xylariales incertae sedis; Monosporascus; unclassified Monosporascus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11997 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q4YHI6|A0A4Q4YHI6_9PEZI ABC1 domain-containing protein OS=Monosporascus sp. CRB-9-2 OX=2211643 GN=DL770_007698 PE=4 SV=1
MM1 pKa = 7.27NRR3 pKa = 11.84LLVLFTCLLAAGNASPFVPDD23 pKa = 3.92IKK25 pKa = 10.87PRR27 pKa = 11.84DD28 pKa = 3.55ICDD31 pKa = 3.33GNTATTRR38 pKa = 11.84TEE40 pKa = 3.42WCDD43 pKa = 3.17FDD45 pKa = 6.49INTDD49 pKa = 3.63YY50 pKa = 11.5YY51 pKa = 10.37NTVPEE56 pKa = 4.23TGVTRR61 pKa = 11.84EE62 pKa = 4.15YY63 pKa = 10.76FFEE66 pKa = 4.48LQDD69 pKa = 3.59VTVSPDD75 pKa = 3.1GVSRR79 pKa = 11.84SAIAVNGSIPGPTIFADD96 pKa = 3.05WGDD99 pKa = 3.8TVVVHH104 pKa = 5.46VTNSLTTSGNGSSIHH119 pKa = 5.28FHH121 pKa = 7.33GIRR124 pKa = 11.84QNFTNQNDD132 pKa = 4.22GVVSITQCPTAVGDD146 pKa = 3.54ATTYY150 pKa = 7.44TWRR153 pKa = 11.84ATQYY157 pKa = 10.72GSTWYY162 pKa = 9.69HH163 pKa = 4.67SHH165 pKa = 7.29FALQAWEE172 pKa = 4.54GVFGGIIINGPASSNYY188 pKa = 10.4DD189 pKa = 3.28EE190 pKa = 5.03DD191 pKa = 4.78LGMVFLNDD199 pKa = 3.43WSHH202 pKa = 4.29QTVDD206 pKa = 3.89EE207 pKa = 5.1LYY209 pKa = 7.78MTAQTSGPPTLDD221 pKa = 2.86NGLINGTNVFGSDD234 pKa = 4.83DD235 pKa = 4.24SDD237 pKa = 3.85DD238 pKa = 3.79QTGSRR243 pKa = 11.84LEE245 pKa = 4.01LSVTEE250 pKa = 4.13GTSYY254 pKa = 11.26RR255 pKa = 11.84LRR257 pKa = 11.84LVNAAIDD264 pKa = 3.54THH266 pKa = 6.65FKK268 pKa = 10.84FSVDD272 pKa = 2.97NHH274 pKa = 5.46TLTVIGMDD282 pKa = 3.86LVPIEE287 pKa = 4.83PYY289 pKa = 7.93TTEE292 pKa = 3.9FVNIGIADD300 pKa = 3.88QATVATDD307 pKa = 3.28FWMRR311 pKa = 11.84AIPQVACSDD320 pKa = 3.55NDD322 pKa = 3.8SADD325 pKa = 3.49NIMAIVHH332 pKa = 6.14YY333 pKa = 9.85GDD335 pKa = 3.59STATPSTTAFEE346 pKa = 4.49YY347 pKa = 10.64TDD349 pKa = 3.47SCDD352 pKa = 5.76DD353 pKa = 3.67EE354 pKa = 5.74DD355 pKa = 4.67LSKK358 pKa = 10.95LVPIVSKK365 pKa = 11.08AVGDD369 pKa = 4.1DD370 pKa = 3.91SNDD373 pKa = 3.44LLEE376 pKa = 4.48IATVGAVGNLFKK388 pKa = 10.64WFLNSTTMVVDD399 pKa = 3.56WADD402 pKa = 3.42PTLLQLSNNITTFDD416 pKa = 4.04DD417 pKa = 3.36SDD419 pKa = 4.05AVIEE423 pKa = 5.36LPDD426 pKa = 4.03ADD428 pKa = 3.28EE429 pKa = 3.91WVYY432 pKa = 11.28VVIQTTFAVPHH443 pKa = 7.1PIHH446 pKa = 6.48LHH448 pKa = 5.3GFDD451 pKa = 4.77FFVLAQGTGTFSSDD465 pKa = 3.06SVTLNTDD472 pKa = 3.01NPPRR476 pKa = 11.84RR477 pKa = 11.84DD478 pKa = 3.33TAMLPASGYY487 pKa = 10.47LVLAFEE493 pKa = 4.81TDD495 pKa = 3.92NPGAWLMHH503 pKa = 5.82CHH505 pKa = 6.47IGWHH509 pKa = 4.85TSEE512 pKa = 4.88GFALQFVVRR521 pKa = 11.84RR522 pKa = 11.84DD523 pKa = 3.8EE524 pKa = 4.26ILDD527 pKa = 4.87LIDD530 pKa = 3.63STTLEE535 pKa = 4.53DD536 pKa = 3.5TCSAWSSYY544 pKa = 11.3AEE546 pKa = 4.08EE547 pKa = 4.64SSVEE551 pKa = 3.95QDD553 pKa = 3.26DD554 pKa = 4.42SGVV557 pKa = 3.12

Molecular weight: 60.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q4WBM7|A0A4Q4WBM7_9PEZI Uncharacterized protein OS=Monosporascus sp. CRB-9-2 OX=2211643 GN=DL770_011809 PE=4 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84RR3 pKa = 11.84ARR5 pKa = 11.84SRR7 pKa = 11.84KK8 pKa = 7.05TSPSGVRR15 pKa = 11.84LMRR18 pKa = 11.84RR19 pKa = 11.84VVRR22 pKa = 11.84VTRR25 pKa = 11.84GMPMRR30 pKa = 11.84ASICARR36 pKa = 11.84RR37 pKa = 11.84LLTAGVVMPRR47 pKa = 11.84LRR49 pKa = 11.84AAALRR54 pKa = 11.84LPAVASATKK63 pKa = 9.84KK64 pKa = 10.09PSSAEE69 pKa = 4.01PISNRR74 pKa = 11.84VHH76 pKa = 6.44AA77 pKa = 5.21

Molecular weight: 8.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11997

0

11997

5711890

66

4747

476.1

52.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.294 ± 0.021

1.17 ± 0.008

5.907 ± 0.014

6.359 ± 0.022

3.537 ± 0.014

7.306 ± 0.022

2.334 ± 0.01

4.503 ± 0.014

4.586 ± 0.02

8.754 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.008

3.508 ± 0.012

6.205 ± 0.026

3.873 ± 0.017

6.592 ± 0.022

7.815 ± 0.021

5.771 ± 0.013

6.202 ± 0.015

1.465 ± 0.008

2.709 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski