Synechococcus phage Bellamy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Bellamyvirus; Synechococcus virus Bellamy

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222YVY0|A0A222YVY0_9CAUD Clamp loader subunit OS=Synechococcus phage Bellamy OX=2023996 GN=156 PE=4 SV=1
MM1 pKa = 7.56YY2 pKa = 10.42ILTIYY7 pKa = 10.49GKK9 pKa = 7.69EE10 pKa = 4.08TEE12 pKa = 4.35GAYY15 pKa = 10.34SVADD19 pKa = 3.91DD20 pKa = 4.63EE21 pKa = 5.34GDD23 pKa = 3.41QILYY27 pKa = 10.46LFEE30 pKa = 5.22EE31 pKa = 4.85EE32 pKa = 4.29DD33 pKa = 3.44DD34 pKa = 3.81AMRR37 pKa = 11.84YY38 pKa = 10.64AMMLEE43 pKa = 4.74DD44 pKa = 5.5DD45 pKa = 5.28GSPDD49 pKa = 3.02MHH51 pKa = 8.09VIEE54 pKa = 4.93VEE56 pKa = 3.94DD57 pKa = 4.28EE58 pKa = 4.39IMIKK62 pKa = 8.19TCEE65 pKa = 3.85MHH67 pKa = 6.85DD68 pKa = 3.56YY69 pKa = 10.21KK70 pKa = 11.34YY71 pKa = 11.31AVITKK76 pKa = 9.13NDD78 pKa = 3.65LVIPPDD84 pKa = 3.71ASHH87 pKa = 7.52DD88 pKa = 3.97FVV90 pKa = 4.93

Molecular weight: 10.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222YW79|A0A222YW79_9CAUD Uncharacterized protein OS=Synechococcus phage Bellamy OX=2023996 GN=265 PE=4 SV=1
MM1 pKa = 7.0NTLNLIRR8 pKa = 11.84KK9 pKa = 8.17QINKK13 pKa = 9.96ASALHH18 pKa = 6.65DD19 pKa = 3.73AQITHH24 pKa = 5.73TAYY27 pKa = 10.45RR28 pKa = 11.84GIVTKK33 pKa = 9.29VTNVKK38 pKa = 8.31PTEE41 pKa = 3.88VHH43 pKa = 6.81GEE45 pKa = 3.61FTYY48 pKa = 10.46RR49 pKa = 11.84GRR51 pKa = 11.84TYY53 pKa = 10.83TKK55 pKa = 10.57

Molecular weight: 6.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

269

0

269

65604

26

4067

243.9

26.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.58 ± 0.189

0.919 ± 0.075

6.495 ± 0.12

6.216 ± 0.237

4.375 ± 0.118

9.156 ± 0.559

1.66 ± 0.101

6.276 ± 0.172

6.318 ± 0.315

6.966 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.184 ± 0.143

5.516 ± 0.131

4.14 ± 0.141

3.267 ± 0.081

4.003 ± 0.139

6.83 ± 0.233

7.178 ± 0.384

6.929 ± 0.199

0.998 ± 0.071

3.992 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski