Dichelobacter nodosus (strain VCS1703A)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cardiobacteriales; Cardiobacteriaceae; Dichelobacter; Dichelobacter nodosus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5EVV8|A5EVV8_DICNV Conserved hypothetical membrane protein OS=Dichelobacter nodosus (strain VCS1703A) OX=246195 GN=DNO_0427 PE=4 SV=1
MM1 pKa = 7.75SDD3 pKa = 3.7FSRR6 pKa = 11.84IQLLADD12 pKa = 3.35SQVIEE17 pKa = 4.14EE18 pKa = 4.26HH19 pKa = 6.45RR20 pKa = 11.84FPLFEE25 pKa = 4.2YY26 pKa = 9.64VGLYY30 pKa = 7.68QRR32 pKa = 11.84CYY34 pKa = 9.76SPEE37 pKa = 3.5HH38 pKa = 6.1KK39 pKa = 9.44MYY41 pKa = 10.83YY42 pKa = 9.77SVIVVALEE50 pKa = 4.03DD51 pKa = 3.76TVGVSDD57 pKa = 4.25EE58 pKa = 4.26KK59 pKa = 11.39LLNLVAASGYY69 pKa = 10.71VDD71 pKa = 5.04DD72 pKa = 6.55DD73 pKa = 4.28SDD75 pKa = 3.3ITFRR79 pKa = 11.84INQASGYY86 pKa = 9.48FYY88 pKa = 10.28TYY90 pKa = 11.03FNFLPDD96 pKa = 3.5APKK99 pKa = 10.95DD100 pKa = 3.7DD101 pKa = 4.19DD102 pKa = 3.87HH103 pKa = 8.44AA104 pKa = 6.78

Molecular weight: 11.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5EY36|A5EY36_DICNV ANK_REP_REGION domain-containing protein OS=Dichelobacter nodosus (strain VCS1703A) OX=246195 GN=DNO_0956 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLSRR13 pKa = 11.84KK14 pKa = 7.51RR15 pKa = 11.84THH17 pKa = 6.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.29NGRR29 pKa = 11.84QVLSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.12GRR40 pKa = 11.84YY41 pKa = 8.65RR42 pKa = 11.84LTVV45 pKa = 2.95

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1273

0

1273

416876

31

2762

327.5

36.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.044 ± 0.092

1.129 ± 0.025

5.347 ± 0.073

6.12 ± 0.075

4.216 ± 0.05

6.359 ± 0.072

2.586 ± 0.036

6.76 ± 0.059

5.047 ± 0.061

10.435 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.438 ± 0.03

3.873 ± 0.047

4.042 ± 0.045

4.957 ± 0.067

5.496 ± 0.067

5.506 ± 0.05

5.087 ± 0.042

6.282 ± 0.063

1.165 ± 0.028

3.111 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski