Alternaria alternata (Alternaria rot fungus) (Torula alternata)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta;

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13463 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177DB46|A0A177DB46_ALTAL FAD/FMN-containing isoamyl alcohol oxidase-like protein MreA OS=Alternaria alternata OX=5599 GN=CC77DRAFT_402917 PE=3 SV=1
MM1 pKa = 7.16RR2 pKa = 11.84QSIAILSILASAACAQDD19 pKa = 3.41VVSFFFPGGYY29 pKa = 10.28DD30 pKa = 3.25GVDD33 pKa = 3.44PVATINTVNPSTTEE47 pKa = 3.45FHH49 pKa = 6.68IACPTDD55 pKa = 3.25TDD57 pKa = 5.35DD58 pKa = 6.17SDD60 pKa = 3.66EE61 pKa = 4.95CGWGPGLDD69 pKa = 3.62VTILSQTRR77 pKa = 11.84FQAQMSTDD85 pKa = 3.69VVSMSLGCDD94 pKa = 3.47YY95 pKa = 11.34NEE97 pKa = 4.72KK98 pKa = 10.17AVEE101 pKa = 4.22MTCTVNQVGGNDD113 pKa = 3.61DD114 pKa = 3.68TGGEE118 pKa = 4.6PITATLSSDD127 pKa = 3.44DD128 pKa = 4.02VKK130 pKa = 10.98FLSATVVAGNSLLTDD145 pKa = 3.88AGSAISSMTEE155 pKa = 3.91FAQGGSTIATTTRR168 pKa = 11.84SASAGLMTPSGSVMPTSTMASASEE192 pKa = 4.11ATSAASGSSSASVSAPEE209 pKa = 4.09STGAATKK216 pKa = 10.65YY217 pKa = 10.34GIEE220 pKa = 4.17GSVLLALAGAAALNVWW236 pKa = 3.62

Molecular weight: 23.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177DED9|A0A177DED9_ALTAL Uncharacterized protein OS=Alternaria alternata OX=5599 GN=CC77DRAFT_1022357 PE=4 SV=1
MM1 pKa = 7.54PLARR5 pKa = 11.84RR6 pKa = 11.84HH7 pKa = 5.56APRR10 pKa = 11.84TAVRR14 pKa = 11.84TSPRR18 pKa = 11.84TTRR21 pKa = 11.84AARR24 pKa = 11.84PSLKK28 pKa = 9.55TRR30 pKa = 11.84LLGGNRR36 pKa = 11.84RR37 pKa = 11.84THH39 pKa = 5.35TKK41 pKa = 8.8RR42 pKa = 11.84TGGTTVTTTTTTRR55 pKa = 11.84TTRR58 pKa = 11.84TGGHH62 pKa = 6.68HH63 pKa = 6.16GHH65 pKa = 6.94ATTAAPVHH73 pKa = 5.12HH74 pKa = 7.32HH75 pKa = 6.33KK76 pKa = 10.63RR77 pKa = 11.84HH78 pKa = 4.81ATMGDD83 pKa = 3.6KK84 pKa = 11.07VSGALMKK91 pKa = 10.77LRR93 pKa = 11.84GSLTRR98 pKa = 11.84RR99 pKa = 11.84PGLKK103 pKa = 10.1AAGTRR108 pKa = 11.84RR109 pKa = 11.84MHH111 pKa = 5.92GTDD114 pKa = 2.72GRR116 pKa = 11.84NARR119 pKa = 11.84RR120 pKa = 11.84VYY122 pKa = 10.89

Molecular weight: 13.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13463

0

13463

6148114

50

7187

456.7

50.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.614 ± 0.018

1.29 ± 0.009

5.746 ± 0.014

6.15 ± 0.024

3.699 ± 0.011

6.743 ± 0.02

2.441 ± 0.009

4.895 ± 0.016

5.038 ± 0.02

8.586 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.293 ± 0.009

3.77 ± 0.013

5.968 ± 0.024

4.085 ± 0.016

5.99 ± 0.018

8.054 ± 0.024

6.143 ± 0.013

6.073 ± 0.014

1.525 ± 0.008

2.895 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski