Pseudomonas phage Dobby

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Citexvirus; Pseudomonas virus Dobby

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F159|A0A3G8F159_9CAUD Holin family protein OS=Pseudomonas phage Dobby OX=2483611 PE=4 SV=1
MM1 pKa = 7.23MMLLGFTAIQQATPPTLEE19 pKa = 4.15PLQGIEE25 pKa = 3.95MASNGVIHH33 pKa = 6.71NNYY36 pKa = 8.66TNYY39 pKa = 10.33TYY41 pKa = 11.06DD42 pKa = 3.28FSSSVSASLHH52 pKa = 5.14TDD54 pKa = 2.78EE55 pKa = 5.65HH56 pKa = 7.12LSLEE60 pKa = 4.42AATSAFSSRR69 pKa = 11.84LASMQEE75 pKa = 3.59PLEE78 pKa = 4.08YY79 pKa = 10.05EE80 pKa = 3.99FSKK83 pKa = 10.78ILSDD87 pKa = 4.38NFLDD91 pKa = 4.88LLAA94 pKa = 5.91

Molecular weight: 10.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F1Q7|A0A3G8F1Q7_9CAUD Tail X protein OS=Pseudomonas phage Dobby OX=2483611 PE=4 SV=1
MM1 pKa = 7.41RR2 pKa = 11.84RR3 pKa = 11.84SRR5 pKa = 11.84KK6 pKa = 9.35RR7 pKa = 11.84KK8 pKa = 8.87HH9 pKa = 6.06NPHH12 pKa = 6.56IPQHH16 pKa = 6.34IDD18 pKa = 2.83QAAIPAAVFFDD29 pKa = 3.54HH30 pKa = 7.22RR31 pKa = 11.84GNGVWYY37 pKa = 8.22TLHH40 pKa = 7.06YY41 pKa = 10.92DD42 pKa = 2.96EE43 pKa = 6.25GGRR46 pKa = 11.84QRR48 pKa = 11.84RR49 pKa = 11.84TNLAPSYY56 pKa = 8.75VTLSEE61 pKa = 3.59LHH63 pKa = 6.75RR64 pKa = 11.84IMEE67 pKa = 4.16EE68 pKa = 3.87RR69 pKa = 11.84EE70 pKa = 4.32GINRR74 pKa = 11.84DD75 pKa = 3.3SLANLCGEE83 pKa = 4.48FHH85 pKa = 7.67KK86 pKa = 10.46STQFKK91 pKa = 10.11RR92 pKa = 11.84LKK94 pKa = 10.37PKK96 pKa = 8.63TQSDD100 pKa = 4.28YY101 pKa = 10.14EE102 pKa = 4.25YY103 pKa = 10.83CRR105 pKa = 11.84EE106 pKa = 3.94VLLAIPTRR114 pKa = 11.84LKK116 pKa = 10.88KK117 pKa = 10.23PLGEE121 pKa = 3.74LAVRR125 pKa = 11.84KK126 pKa = 8.71FSPALVQRR134 pKa = 11.84LVDD137 pKa = 5.17RR138 pKa = 11.84IAEE141 pKa = 4.13EE142 pKa = 4.47GTPSKK147 pKa = 10.33AAHH150 pKa = 5.11VLRR153 pKa = 11.84YY154 pKa = 9.43LRR156 pKa = 11.84RR157 pKa = 11.84VMQWGRR163 pKa = 11.84NRR165 pKa = 11.84GYY167 pKa = 10.87LQINVAQGIEE177 pKa = 4.02APVEE181 pKa = 4.01RR182 pKa = 11.84KK183 pKa = 7.39QRR185 pKa = 11.84RR186 pKa = 11.84LPAPTVMYY194 pKa = 10.54RR195 pKa = 11.84LIDD198 pKa = 3.48RR199 pKa = 11.84ARR201 pKa = 11.84EE202 pKa = 3.8LGKK205 pKa = 10.5LKK207 pKa = 10.41RR208 pKa = 11.84GQPGACPAYY217 pKa = 9.37LCSVMEE223 pKa = 4.12LAYY226 pKa = 10.56LCRR229 pKa = 11.84LRR231 pKa = 11.84GIEE234 pKa = 4.31TVTLSDD240 pKa = 4.09ANEE243 pKa = 4.06LPEE246 pKa = 5.71GILTNRR252 pKa = 11.84RR253 pKa = 11.84KK254 pKa = 10.18GSRR257 pKa = 11.84NNVVRR262 pKa = 11.84WTPRR266 pKa = 11.84LRR268 pKa = 11.84AAWDD272 pKa = 3.13HH273 pKa = 5.77AKK275 pKa = 10.42AYY277 pKa = 7.6RR278 pKa = 11.84TQVWTSKK285 pKa = 10.78SLPTPTAPEE294 pKa = 3.93LRR296 pKa = 11.84PIIVASHH303 pKa = 6.44GGSLQKK309 pKa = 10.77SSLDD313 pKa = 3.71SAWQRR318 pKa = 11.84FITAALEE325 pKa = 4.12AGIITPDD332 pKa = 3.28QRR334 pKa = 11.84FGLHH338 pKa = 5.59DD339 pKa = 4.49LKK341 pKa = 11.09RR342 pKa = 11.84RR343 pKa = 11.84GITDD347 pKa = 3.57TPGTRR352 pKa = 11.84ADD354 pKa = 3.4KK355 pKa = 11.14QEE357 pKa = 4.13ASGHH361 pKa = 5.96RR362 pKa = 11.84DD363 pKa = 2.98EE364 pKa = 6.44SMLDD368 pKa = 3.53VYY370 pKa = 11.02DD371 pKa = 4.53LSVPIVSPSADD382 pKa = 2.92

Molecular weight: 43.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11281

38

919

230.2

25.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.338 ± 0.508

1.011 ± 0.135

5.984 ± 0.235

5.664 ± 0.347

3.041 ± 0.28

7.526 ± 0.423

2.003 ± 0.173

5.168 ± 0.282

4.166 ± 0.335

10.673 ± 0.419

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.127 ± 0.185

3.227 ± 0.212

4.547 ± 0.28

4.982 ± 0.29

7.021 ± 0.351

5.833 ± 0.372

5.718 ± 0.299

5.611 ± 0.296

1.667 ± 0.162

2.695 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski