Microbacterium phage LeeroyJenkins

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514TXW8|A0A514TXW8_9CAUD Uncharacterized protein OS=Microbacterium phage LeeroyJenkins OX=2593338 GN=130 PE=4 SV=1
MM1 pKa = 7.28NASEE5 pKa = 4.28YY6 pKa = 10.56EE7 pKa = 4.22NYY9 pKa = 10.4SEE11 pKa = 6.21AIAAYY16 pKa = 10.26DD17 pKa = 3.48EE18 pKa = 4.75ALNDD22 pKa = 3.42HH23 pKa = 6.68YY24 pKa = 11.24FPVIAGGIEE33 pKa = 3.97FDD35 pKa = 3.05RR36 pKa = 11.84SRR38 pKa = 11.84ILRR41 pKa = 11.84EE42 pKa = 4.02LDD44 pKa = 3.92PIAYY48 pKa = 9.32RR49 pKa = 11.84VGFFDD54 pKa = 3.28WLDD57 pKa = 3.61AEE59 pKa = 5.87GIDD62 pKa = 5.74SDD64 pKa = 5.2DD65 pKa = 5.54LDD67 pKa = 5.77DD68 pKa = 5.44EE69 pKa = 5.89GYY71 pKa = 11.2DD72 pKa = 4.41DD73 pKa = 6.2LPP75 pKa = 5.96

Molecular weight: 8.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514TXV8|A0A514TXV8_9CAUD HNH endonuclease OS=Microbacterium phage LeeroyJenkins OX=2593338 GN=114 PE=4 SV=1
MM1 pKa = 7.45NNNNKK6 pKa = 10.16GIRR9 pKa = 11.84NGVTYY14 pKa = 9.33TLNGEE19 pKa = 4.14HH20 pKa = 6.87VEE22 pKa = 3.86IRR24 pKa = 11.84TADD27 pKa = 3.19STNRR31 pKa = 11.84VRR33 pKa = 11.84IMRR36 pKa = 11.84TARR39 pKa = 11.84AKK41 pKa = 10.25GATDD45 pKa = 2.81ITVYY49 pKa = 10.53TNGRR53 pKa = 11.84SLVADD58 pKa = 4.4WSS60 pKa = 3.79

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

125

0

125

18781

40

1085

150.2

16.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.621 ± 0.608

0.932 ± 0.172

6.842 ± 0.262

7.279 ± 0.339

3.509 ± 0.152

8.407 ± 0.305

1.571 ± 0.144

4.883 ± 0.154

4.105 ± 0.22

7.396 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.311 ± 0.121

3.871 ± 0.194

4.27 ± 0.2

3.147 ± 0.232

6.342 ± 0.269

6.23 ± 0.242

6.608 ± 0.287

6.98 ± 0.217

2.162 ± 0.143

3.535 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski