Burkholderia phage PE067

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5S8Y0L0|A0A5S8Y0L0_9CAUD Uncharacterized protein OS=Burkholderia phage PE067 OX=1735698 GN=PE067_060 PE=4 SV=1
MM1 pKa = 6.72TTQPMLQKK9 pKa = 10.2IPVVRR14 pKa = 11.84DD15 pKa = 3.04EE16 pKa = 4.32NGYY19 pKa = 10.16FIHH22 pKa = 7.94PDD24 pKa = 3.4LLHH27 pKa = 6.56FWTVTMDD34 pKa = 3.72GAEE37 pKa = 4.17HH38 pKa = 6.34CTPEE42 pKa = 3.53QWATLEE48 pKa = 4.23SKK50 pKa = 10.87AGIKK54 pKa = 9.38TSIYY58 pKa = 10.09HH59 pKa = 6.62LEE61 pKa = 4.24SEE63 pKa = 4.64NLDD66 pKa = 3.3HH67 pKa = 7.18PAYY70 pKa = 10.59VSYY73 pKa = 10.78FDD75 pKa = 5.29NGNLDD80 pKa = 3.15ITEE83 pKa = 4.37WDD85 pKa = 4.01PSPEE89 pKa = 4.25PGWWLLEE96 pKa = 4.36IGDD99 pKa = 4.83SEE101 pKa = 4.7DD102 pKa = 3.31GPYY105 pKa = 10.84AVYY108 pKa = 8.7ATHH111 pKa = 7.43AA112 pKa = 3.99

Molecular weight: 12.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G7GJI8|A0A6G7GJI8_9CAUD ATP-dependent protease proteolytic subunit OS=Burkholderia phage PE067 OX=1735698 GN=PE067_029 PE=4 SV=1
MM1 pKa = 7.73LDD3 pKa = 2.99IFQVVTHH10 pKa = 6.2CLITLFKK17 pKa = 10.42HH18 pKa = 6.1RR19 pKa = 11.84NPIRR23 pKa = 11.84KK24 pKa = 8.45PRR26 pKa = 11.84IKK28 pKa = 10.71ANTPRR33 pKa = 11.84RR34 pKa = 11.84SPYY37 pKa = 10.34QRR39 pKa = 11.84GQDD42 pKa = 3.59GNEE45 pKa = 3.79TGDD48 pKa = 3.53RR49 pKa = 11.84TNHH52 pKa = 5.91GFCTYY57 pKa = 10.47KK58 pKa = 10.48PRR60 pKa = 11.84TGAILLVILTIHH72 pKa = 7.11LIWPVNN78 pKa = 3.36

Molecular weight: 9.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

13885

43

678

201.2

21.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.345 ± 0.534

0.922 ± 0.106

5.596 ± 0.302

5.488 ± 0.56

3.032 ± 0.206

7.879 ± 0.384

1.534 ± 0.145

5.092 ± 0.219

3.615 ± 0.313

8.102 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.113

3.637 ± 0.236

5.243 ± 0.266

4.696 ± 0.293

6.403 ± 0.445

5.877 ± 0.453

6.129 ± 0.38

6.972 ± 0.25

1.505 ± 0.133

2.593 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski