Tomato mottle virus (isolate Florida) (ToMoV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Tomato mottle virus

Average proteome isoelectric point is 8.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q06658|TRAP_TMOV Transcriptional activator protein OS=Tomato mottle virus (isolate Florida) OX=223359 GN=AC2 PE=1 SV=1
MM1 pKa = 7.88PPPKK5 pKa = 10.09KK6 pKa = 10.36FRR8 pKa = 11.84VQSKK12 pKa = 10.58NYY14 pKa = 9.23FLTYY18 pKa = 8.54PQCSLSKK25 pKa = 11.0EE26 pKa = 4.09EE27 pKa = 5.81ALSQLQNLNTPVNKK41 pKa = 10.12KK42 pKa = 9.89FIKK45 pKa = 9.67ICRR48 pKa = 11.84EE49 pKa = 3.52LHH51 pKa = 6.26EE52 pKa = 4.93NGEE55 pKa = 4.29PHH57 pKa = 6.86LHH59 pKa = 5.98VLVQFEE65 pKa = 4.74GKK67 pKa = 8.79YY68 pKa = 9.24QCTNNRR74 pKa = 11.84FFDD77 pKa = 4.02LVSPTRR83 pKa = 11.84SAHH86 pKa = 4.86FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.8KK106 pKa = 11.28DD107 pKa = 3.26GDD109 pKa = 3.84TIEE112 pKa = 4.54WGDD115 pKa = 3.55FQIDD119 pKa = 3.22GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQSANDD132 pKa = 3.98SYY134 pKa = 12.05AKK136 pKa = 10.43ALNAGSVQSALAVLRR151 pKa = 11.84EE152 pKa = 4.07EE153 pKa = 4.17QPKK156 pKa = 11.06DD157 pKa = 3.67FVLQNHH163 pKa = 6.93NIRR166 pKa = 11.84SNLEE170 pKa = 3.88RR171 pKa = 11.84IFAKK175 pKa = 10.49APEE178 pKa = 4.22PWVPPFQVSSFTNVPDD194 pKa = 5.1EE195 pKa = 4.15MQEE198 pKa = 3.71WADD201 pKa = 3.64NYY203 pKa = 10.84FGTGDD208 pKa = 3.93AAPPDD213 pKa = 4.03RR214 pKa = 11.84PVSIIVEE221 pKa = 4.27GDD223 pKa = 3.08SRR225 pKa = 11.84TGKK228 pKa = 8.55TMWARR233 pKa = 11.84ALGPHH238 pKa = 6.63NYY240 pKa = 10.14LSGHH244 pKa = 6.79LDD246 pKa = 3.21FNGRR250 pKa = 11.84VFSNDD255 pKa = 2.42VQYY258 pKa = 11.74NVIDD262 pKa = 5.36DD263 pKa = 4.14IAPHH267 pKa = 5.05YY268 pKa = 10.73LKK270 pKa = 10.71LKK272 pKa = 9.14HH273 pKa = 5.83WKK275 pKa = 9.52EE276 pKa = 3.61LLGAQKK282 pKa = 10.6DD283 pKa = 3.68WQSNCKK289 pKa = 9.38YY290 pKa = 10.21GKK292 pKa = 9.18PVQIKK297 pKa = 9.93GGIPAIVLCNPGEE310 pKa = 4.11GASYY314 pKa = 11.25KK315 pKa = 10.62EE316 pKa = 4.18FLDD319 pKa = 3.62KK320 pKa = 11.54AEE322 pKa = 4.1NTGLKK327 pKa = 9.9NWTIKK332 pKa = 10.6NAIFITLTAPLYY344 pKa = 10.41QEE346 pKa = 4.27STQASQEE353 pKa = 4.12TGNQKK358 pKa = 10.43AQGG361 pKa = 3.58

Molecular weight: 40.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q06657|REP_TMOV Replication-associated protein OS=Tomato mottle virus (isolate Florida) OX=223359 GN=AC1 PE=3 SV=1
MM1 pKa = 8.04PKK3 pKa = 9.68RR4 pKa = 11.84DD5 pKa = 3.86LPWRR9 pKa = 11.84SMAGTSKK16 pKa = 10.56VSRR19 pKa = 11.84NANYY23 pKa = 10.32SPRR26 pKa = 11.84AGIGPRR32 pKa = 11.84INKK35 pKa = 8.66AAEE38 pKa = 3.81WVNRR42 pKa = 11.84PMYY45 pKa = 9.9RR46 pKa = 11.84KK47 pKa = 8.78PRR49 pKa = 11.84MYY51 pKa = 9.44RR52 pKa = 11.84TLRR55 pKa = 11.84TTDD58 pKa = 3.06VARR61 pKa = 11.84GCEE64 pKa = 4.62GPCKK68 pKa = 10.21VQSFEE73 pKa = 3.82QRR75 pKa = 11.84HH76 pKa = 6.24DD77 pKa = 3.0ISHH80 pKa = 6.95IGKK83 pKa = 9.36VMCISDD89 pKa = 3.56VTRR92 pKa = 11.84GNGITHH98 pKa = 6.78RR99 pKa = 11.84VGKK102 pKa = 9.44RR103 pKa = 11.84FCVKK107 pKa = 9.92SVYY110 pKa = 10.16ILGKK114 pKa = 9.37IWMDD118 pKa = 3.39EE119 pKa = 4.0NIKK122 pKa = 10.61LKK124 pKa = 10.73NHH126 pKa = 5.95TNSVMFWLVRR136 pKa = 11.84DD137 pKa = 3.7RR138 pKa = 11.84RR139 pKa = 11.84PYY141 pKa = 8.14GTPMDD146 pKa = 3.06IWTVFNMFDD155 pKa = 4.22NEE157 pKa = 4.11PSTATVKK164 pKa = 10.81NDD166 pKa = 2.84LRR168 pKa = 11.84DD169 pKa = 3.64RR170 pKa = 11.84YY171 pKa = 10.2QVMHH175 pKa = 6.91KK176 pKa = 10.25FYY178 pKa = 11.12GKK180 pKa = 8.61VTGGQYY186 pKa = 11.07ASNEE190 pKa = 3.69QAIVKK195 pKa = 9.05RR196 pKa = 11.84FWKK199 pKa = 10.22VNNHH203 pKa = 4.48VVYY206 pKa = 10.67NHH208 pKa = 5.8QEE210 pKa = 3.47AGKK213 pKa = 10.41YY214 pKa = 8.39EE215 pKa = 4.09NHH217 pKa = 6.52TEE219 pKa = 3.99NALLLYY225 pKa = 7.29MACTHH230 pKa = 7.07ASNPVYY236 pKa = 9.86ATLKK240 pKa = 9.47IRR242 pKa = 11.84IYY244 pKa = 10.59FYY246 pKa = 11.38DD247 pKa = 4.2SITNN251 pKa = 3.6

Molecular weight: 29.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1423

129

361

237.2

27.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.411 ± 0.719

1.757 ± 0.241

5.06 ± 0.321

4.638 ± 0.419

4.498 ± 0.42

5.903 ± 0.465

3.795 ± 0.351

5.622 ± 0.495

6.395 ± 0.533

6.887 ± 0.632

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.178 ± 0.514

5.973 ± 0.719

5.2 ± 0.511

4.146 ± 0.859

6.957 ± 1.0

8.222 ± 1.051

5.06 ± 0.424

6.676 ± 0.708

1.616 ± 0.26

4.006 ± 0.51
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski