Leptospira weilii str. Ecochallenge

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Leptospirales; Leptospiraceae; Leptospira; Leptospira weilii

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4702 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N1U5Y3|N1U5Y3_9LEPT Transcription termination factor Rho OS=Leptospira weilii str. Ecochallenge OX=1049986 GN=rho PE=3 SV=1
MM1 pKa = 7.51SGAFLGDD8 pKa = 3.62FEE10 pKa = 6.47SFFLHH15 pKa = 7.04SLQEE19 pKa = 4.21DD20 pKa = 3.92CMNFYY25 pKa = 11.18DD26 pKa = 5.0FDD28 pKa = 4.11PVAAKK33 pKa = 10.05VLPII37 pKa = 4.22

Molecular weight: 4.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N1U5M3|N1U5M3_9LEPT Uncharacterized protein OS=Leptospira weilii str. Ecochallenge OX=1049986 GN=LEP1GSC043_1076 PE=4 SV=1
MM1 pKa = 7.55SLIFNPFFLKK11 pKa = 8.61YY12 pKa = 9.49HH13 pKa = 6.47RR14 pKa = 11.84SIFEE18 pKa = 4.37SNSSEE23 pKa = 3.82FLPVLEE29 pKa = 5.03RR30 pKa = 11.84SLFYY34 pKa = 10.24IAHH37 pKa = 7.24LIFAKK42 pKa = 9.52SLRR45 pKa = 11.84LAQPRR50 pKa = 11.84FYY52 pKa = 10.94GRR54 pKa = 11.84SVTLRR59 pKa = 11.84WLRR62 pKa = 11.84LSRR65 pKa = 11.84SLRR68 pKa = 11.84IAHH71 pKa = 7.04LSFAKK76 pKa = 10.02SLRR79 pKa = 11.84LAQPRR84 pKa = 11.84FYY86 pKa = 10.83GRR88 pKa = 11.84SVMLRR93 pKa = 11.84WLRR96 pKa = 11.84LSRR99 pKa = 11.84SLRR102 pKa = 11.84IAHH105 pKa = 7.04LSFAKK110 pKa = 10.18SLRR113 pKa = 11.84LTLRR117 pKa = 11.84WLRR120 pKa = 11.84LSRR123 pKa = 11.84SLRR126 pKa = 11.84IAHH129 pKa = 7.04LSFAKK134 pKa = 10.18SLRR137 pKa = 11.84LTLRR141 pKa = 11.84WLRR144 pKa = 11.84LSRR147 pKa = 11.84SLRR150 pKa = 11.84IAHH153 pKa = 7.04LSFAKK158 pKa = 10.18SLRR161 pKa = 11.84LTLRR165 pKa = 11.84WLRR168 pKa = 11.84LSRR171 pKa = 11.84SLRR174 pKa = 11.84IAHH177 pKa = 7.04LSFAKK182 pKa = 10.18SLRR185 pKa = 11.84LTLRR189 pKa = 11.84WLRR192 pKa = 11.84LSRR195 pKa = 11.84SLRR198 pKa = 11.84IAHH201 pKa = 7.04LSFAKK206 pKa = 10.18SLRR209 pKa = 11.84LTLRR213 pKa = 11.84WLRR216 pKa = 11.84LSRR219 pKa = 11.84SLRR222 pKa = 11.84IARR225 pKa = 4.08

Molecular weight: 27.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4702

0

4702

1108066

32

2738

235.7

26.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.592 ± 0.042

0.878 ± 0.013

4.761 ± 0.027

7.158 ± 0.046

5.79 ± 0.039

6.557 ± 0.037

1.684 ± 0.015

7.667 ± 0.037

7.673 ± 0.042

10.31 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.996 ± 0.015

4.78 ± 0.029

3.963 ± 0.025

3.176 ± 0.027

4.988 ± 0.03

7.783 ± 0.041

4.903 ± 0.031

5.74 ± 0.029

1.095 ± 0.016

3.507 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski