Listeria floridensis FSL S10-1187

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria; Listeria floridensis

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2904 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|W7CAD2|W7CAD2_9LIST Uncharacterized protein OS=Listeria floridensis FSL S10-1187 OX=1265817 GN=MFLO_01215 PE=4 SV=1
MM1 pKa = 7.86IDD3 pKa = 3.19GKK5 pKa = 7.93TNRR8 pKa = 11.84FDD10 pKa = 3.93YY11 pKa = 10.7SGCVYY16 pKa = 10.63PIGLVPDD23 pKa = 3.4EE24 pKa = 3.7VLYY27 pKa = 11.0FNEE30 pKa = 4.03EE31 pKa = 4.15NIDD34 pKa = 3.64EE35 pKa = 4.26VLFAGFYY42 pKa = 10.83DD43 pKa = 4.12DD44 pKa = 6.37DD45 pKa = 3.9EE46 pKa = 6.33ARR48 pKa = 11.84FQTLYY53 pKa = 11.25SDD55 pKa = 3.17WSEE58 pKa = 4.1NNASGIEE65 pKa = 3.98KK66 pKa = 10.56GVVDD70 pKa = 5.13KK71 pKa = 11.01PLL73 pKa = 4.56

Molecular weight:
8.34 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|W7C958|W7C958_9LIST 30S ribosomal protein S2 OS=Listeria floridensis FSL S10-1187 OX=1265817 GN=rpsB PE=3 SV=1
MM1 pKa = 7.12HH2 pKa = 7.55RR3 pKa = 11.84CLFFLLYY10 pKa = 10.07FKK12 pKa = 10.55VVKK15 pKa = 10.07RR16 pKa = 11.84GSIALFIVFLTSCGIEE32 pKa = 4.17VVQLIQTTLVTGFSGMTLPSGRR54 pKa = 11.84STDD57 pKa = 3.12IDD59 pKa = 4.84DD60 pKa = 5.44IILNTLSGSLGVLIIYY76 pKa = 7.8LIPNFRR82 pKa = 11.84KK83 pKa = 10.11RR84 pKa = 11.84IGRR87 pKa = 11.84KK88 pKa = 8.56KK89 pKa = 10.31RR90 pKa = 11.84RR91 pKa = 3.46

Molecular weight:
10.28 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2904

0

2904

737768

22

2800

254.1

28.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.9 ± 0.055

0.583 ± 0.013

5.384 ± 0.045

7.376 ± 0.067

4.754 ± 0.054

6.897 ± 0.042

1.79 ± 0.024

7.271 ± 0.049

6.994 ± 0.044

9.747 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.608 ± 0.027

4.296 ± 0.04

3.421 ± 0.028

3.529 ± 0.03

4.241 ± 0.033

5.976 ± 0.033

5.784 ± 0.064

7.086 ± 0.045

0.902 ± 0.019

3.463 ± 0.035

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski