Rattus norvegicus polyomavirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Alphapolyomavirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4PP86|A0A0H4PP86_9POLY Capsid protein VP1 OS=Rattus norvegicus polyomavirus 1 OX=1679933 GN=VP1 PE=3 SV=1
MM1 pKa = 7.29GAAISVIIDD10 pKa = 4.47LIATATEE17 pKa = 4.56LSTATGFTVEE27 pKa = 4.41AVLSGEE33 pKa = 3.88ALAAIEE39 pKa = 4.38AQVSTIATMEE49 pKa = 4.06GLAAEE54 pKa = 4.41SALASMGITPEE65 pKa = 4.0MYY67 pKa = 11.15AMISTVPPTVEE78 pKa = 4.68GIISGAAGTAHH89 pKa = 7.69ILQTIAGSASLTLGVISGFRR109 pKa = 11.84DD110 pKa = 3.45QQVSIVNRR118 pKa = 11.84NMALIPWRR126 pKa = 11.84PEE128 pKa = 3.79EE129 pKa = 4.05YY130 pKa = 9.79WDD132 pKa = 3.86VLFPGVNTIARR143 pKa = 11.84GLDD146 pKa = 3.61VAHH149 pKa = 6.87GWAHH153 pKa = 6.69SLFQSVSRR161 pKa = 11.84QIWRR165 pKa = 11.84TLFEE169 pKa = 4.15EE170 pKa = 4.03TRR172 pKa = 11.84AAIDD176 pKa = 3.45YY177 pKa = 9.81AVRR180 pKa = 11.84DD181 pKa = 3.55VTIRR185 pKa = 11.84QTHH188 pKa = 6.13RR189 pKa = 11.84LLDD192 pKa = 3.69GVARR196 pKa = 11.84MLEE199 pKa = 3.9NTRR202 pKa = 11.84WVVTNAVEE210 pKa = 3.87QGGQIVSDD218 pKa = 3.92AVQNTGQLVLTAPGEE233 pKa = 4.24AYY235 pKa = 10.18RR236 pKa = 11.84GLRR239 pKa = 11.84NYY241 pKa = 9.9YY242 pKa = 10.73AEE244 pKa = 4.5LPGVNPAQRR253 pKa = 11.84QQMLRR258 pKa = 11.84TIEE261 pKa = 4.16RR262 pKa = 11.84EE263 pKa = 3.76NAQQALRR270 pKa = 11.84TEE272 pKa = 4.3VGRR275 pKa = 11.84DD276 pKa = 3.61VQSVTPSPKK285 pKa = 9.85SSGFTEE291 pKa = 4.13SGNVIQRR298 pKa = 11.84YY299 pKa = 5.76MPPGGAMQRR308 pKa = 11.84TCPDD312 pKa = 2.37WMLPLILGLYY322 pKa = 10.36GDD324 pKa = 5.34INPTWHH330 pKa = 6.46SVIEE334 pKa = 4.21EE335 pKa = 4.29EE336 pKa = 5.24YY337 pKa = 10.82GPQKK341 pKa = 10.17KK342 pKa = 9.2RR343 pKa = 11.84RR344 pKa = 11.84RR345 pKa = 11.84LQQ347 pKa = 3.2

Molecular weight: 37.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4PK86|A0A0H4PK86_9POLY Minor capsid protein OS=Rattus norvegicus polyomavirus 1 OX=1679933 GN=VP3 PE=3 SV=1
MM1 pKa = 7.92DD2 pKa = 5.99KK3 pKa = 10.69ILTKK7 pKa = 9.86EE8 pKa = 4.2EE9 pKa = 4.1KK10 pKa = 10.27SQLLALLDD18 pKa = 4.57LDD20 pKa = 3.79GQYY23 pKa = 10.18WGDD26 pKa = 3.82FGRR29 pKa = 11.84MQKK32 pKa = 10.48AYY34 pKa = 9.76HH35 pKa = 5.52AQSLKK40 pKa = 10.25FHH42 pKa = 7.28PDD44 pKa = 2.83KK45 pKa = 11.59GGDD48 pKa = 3.7PLLMQLLNTLWTKK61 pKa = 10.61LKK63 pKa = 10.64HH64 pKa = 5.97GLHH67 pKa = 5.02QVRR70 pKa = 11.84LNLGGHH76 pKa = 5.26EE77 pKa = 4.21VRR79 pKa = 11.84RR80 pKa = 11.84LPEE83 pKa = 4.3GDD85 pKa = 2.47WWLSTRR91 pKa = 11.84QTFGGNYY98 pKa = 8.69SRR100 pKa = 11.84RR101 pKa = 11.84LCRR104 pKa = 11.84MPITCLQNKK113 pKa = 9.21ACSTCNCILCTLRR126 pKa = 11.84RR127 pKa = 11.84QHH129 pKa = 6.94RR130 pKa = 11.84GLKK133 pKa = 9.2KK134 pKa = 10.57ACLAPCLVLGEE145 pKa = 5.05CYY147 pKa = 10.6CLDD150 pKa = 4.78CFSLWFGLPINNFIVVLYY168 pKa = 11.02AEE170 pKa = 4.74FLGDD174 pKa = 5.62IPVDD178 pKa = 3.13WLDD181 pKa = 3.39IDD183 pKa = 3.55VHH185 pKa = 6.81QIYY188 pKa = 10.56NPAVGKK194 pKa = 9.97YY195 pKa = 8.83

Molecular weight: 22.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

2625

95

776

328.1

36.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.829 ± 0.797

2.629 ± 0.666

5.143 ± 0.446

5.371 ± 0.387

3.2 ± 0.714

7.619 ± 0.671

2.667 ± 0.446

4.724 ± 0.578

4.914 ± 0.992

11.086 ± 1.16

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.28

4.571 ± 0.517

6.286 ± 0.522

5.41 ± 0.302

5.524 ± 0.673

6.019 ± 0.484

5.562 ± 0.508

5.6 ± 0.668

2.057 ± 0.226

3.276 ± 0.321
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski