Nitrosovibrio sp. Nv17

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosovibrio; unclassified Nitrosovibrio

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2594 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1K1M9X0|A0A1K1M9X0_9PROT Type IV pilus assembly protein PilA OS=Nitrosovibrio sp. Nv17 OX=1855339 GN=SAMN05216414_10591 PE=3 SV=1
MM1 pKa = 7.54AHH3 pKa = 7.33TIPQTSRR10 pKa = 11.84DD11 pKa = 3.56YY12 pKa = 10.8DD13 pKa = 3.57TTRR16 pKa = 11.84VIEE19 pKa = 4.49RR20 pKa = 11.84PDD22 pKa = 2.84GFYY25 pKa = 9.83WHH27 pKa = 7.8DD28 pKa = 3.65EE29 pKa = 4.3NEE31 pKa = 4.3SEE33 pKa = 4.96RR34 pKa = 11.84VFGPFPTLLEE44 pKa = 4.98AIQDD48 pKa = 3.6MEE50 pKa = 4.54YY51 pKa = 10.75NAEE54 pKa = 3.9SDD56 pKa = 4.15YY57 pKa = 11.59EE58 pKa = 4.18PGEE61 pKa = 4.16TLEE64 pKa = 4.16EE65 pKa = 5.04AEE67 pKa = 4.71DD68 pKa = 4.26EE69 pKa = 4.25IGISGWVDD77 pKa = 3.36QEE79 pKa = 4.92AGEE82 pKa = 4.27SDD84 pKa = 3.79NNLFHH89 pKa = 7.55YY90 pKa = 10.77DD91 pKa = 3.57DD92 pKa = 4.0

Molecular weight: 10.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1K1MNF2|A0A1K1MNF2_9PROT ATP phosphoribosyltransferase OS=Nitrosovibrio sp. Nv17 OX=1855339 GN=hisG PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.04TVGGAAVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.76GRR39 pKa = 11.84ARR41 pKa = 11.84LGVV44 pKa = 3.34

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2594

0

2594

829186

27

3483

319.7

35.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.473 ± 0.064

0.934 ± 0.017

5.509 ± 0.034

5.809 ± 0.051

3.543 ± 0.032

8.457 ± 0.06

2.559 ± 0.026

5.08 ± 0.036

2.954 ± 0.041

10.754 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.441 ± 0.026

2.645 ± 0.026

5.154 ± 0.032

3.568 ± 0.03

7.793 ± 0.048

5.492 ± 0.039

4.924 ± 0.033

7.077 ± 0.042

1.314 ± 0.022

2.52 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski