Burkholderiales bacterium PBB3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; unclassified Burkholderiales

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4253 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257CI22|A0A257CI22_9BURK IS4 family transposase OS=Burkholderiales bacterium PBB3 OX=2015565 GN=CFE43_20950 PE=4 SV=1
MM1 pKa = 7.2NRR3 pKa = 11.84INIMKK8 pKa = 9.23TDD10 pKa = 3.34QCAIEE15 pKa = 4.49SMSDD19 pKa = 2.9SAEE22 pKa = 4.09ALDD25 pKa = 4.06VVVDD29 pKa = 4.13SALASCDD36 pKa = 3.79LDD38 pKa = 3.54PHH40 pKa = 8.0IGAFDD45 pKa = 4.57GYY47 pKa = 11.22DD48 pKa = 3.19VTTEE52 pKa = 3.71FLTIKK57 pKa = 10.6GGSCC61 pKa = 3.72

Molecular weight: 6.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257CX61|A0A257CX61_9BURK ATP-sulfurylase large subunit OS=Burkholderiales bacterium PBB3 OX=2015565 GN=CFE43_01210 PE=4 SV=1
MM1 pKa = 7.91PKK3 pKa = 9.67MKK5 pKa = 9.88TKK7 pKa = 10.49SAAKK11 pKa = 9.4KK12 pKa = 9.52RR13 pKa = 11.84FRR15 pKa = 11.84VRR17 pKa = 11.84PGGTVKK23 pKa = 10.62RR24 pKa = 11.84GQAFKK29 pKa = 11.01RR30 pKa = 11.84HH31 pKa = 5.79ILTKK35 pKa = 9.78KK36 pKa = 3.94TTKK39 pKa = 10.22NKK41 pKa = 8.85RR42 pKa = 11.84HH43 pKa = 5.85LRR45 pKa = 11.84GSTAVHH51 pKa = 5.39STNMGHH57 pKa = 6.4MAQMLPGQGII67 pKa = 3.67

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4253

0

4253

1417406

27

3818

333.3

36.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.203 ± 0.047

0.943 ± 0.013

5.198 ± 0.029

4.941 ± 0.032

3.605 ± 0.022

7.97 ± 0.045

2.191 ± 0.02

4.415 ± 0.03

3.904 ± 0.037

10.709 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.479 ± 0.02

3.019 ± 0.025

5.011 ± 0.028

4.359 ± 0.027

5.971 ± 0.036

5.953 ± 0.035

5.618 ± 0.036

7.629 ± 0.029

1.543 ± 0.016

2.337 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski