Streptococcus phage 7A5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P0VID9|A0A2P0VID9_9CAUD Uncharacterized protein OS=Streptococcus phage 7A5 OX=2041496 GN=7A5_017 PE=4 SV=1
MM1 pKa = 7.3SVSKK5 pKa = 9.08EE6 pKa = 4.26TIMQTLNLDD15 pKa = 3.8EE16 pKa = 4.97TDD18 pKa = 3.78DD19 pKa = 3.8TALIPAYY26 pKa = 9.65IEE28 pKa = 3.9SARR31 pKa = 11.84QYY33 pKa = 10.3VVNSVGDD40 pKa = 3.54DD41 pKa = 3.37PKK43 pKa = 10.95FYY45 pKa = 11.07NLDD48 pKa = 3.41SVRR51 pKa = 11.84ALFDD55 pKa = 3.34TAVIALTSSYY65 pKa = 8.66FTYY68 pKa = 10.07RR69 pKa = 11.84VALTDD74 pKa = 3.37TATYY78 pKa = 9.41PVNLTLNSIIGQLRR92 pKa = 11.84GLYY95 pKa = 8.66ATYY98 pKa = 10.56SEE100 pKa = 4.46EE101 pKa = 5.06RR102 pKa = 11.84GDD104 pKa = 3.53

Molecular weight: 11.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P0VIU3|A0A2P0VIU3_9CAUD Terminase large subunit OS=Streptococcus phage 7A5 OX=2041496 GN=7A5_002 PE=4 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84SKK4 pKa = 11.27YY5 pKa = 9.93KK6 pKa = 10.66DD7 pKa = 3.41KK8 pKa = 11.17LVGIYY13 pKa = 10.22APARR17 pKa = 11.84YY18 pKa = 8.96GHH20 pKa = 6.1TSVLEE25 pKa = 4.05KK26 pKa = 9.96TQEE29 pKa = 3.66FSKK32 pKa = 10.41WFWKK36 pKa = 8.74NHH38 pKa = 5.03KK39 pKa = 10.6DD40 pKa = 4.0IDD42 pKa = 4.9LISNKK47 pKa = 10.36LGISTNKK54 pKa = 9.91LNRR57 pKa = 11.84ILTLEE62 pKa = 3.93QLPDD66 pKa = 3.17EE67 pKa = 4.65RR68 pKa = 11.84LLKK71 pKa = 10.85KK72 pKa = 10.27MIEE75 pKa = 4.03LCKK78 pKa = 10.72

Molecular weight: 9.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

10787

42

1593

245.2

27.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.554 ± 0.497

0.547 ± 0.117

6.424 ± 0.248

6.944 ± 0.5

4.422 ± 0.255

6.267 ± 0.474

1.391 ± 0.112

6.406 ± 0.217

8.575 ± 0.555

7.973 ± 0.41

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.151

6.369 ± 0.274

2.948 ± 0.196

4.209 ± 0.267

4.023 ± 0.274

6.703 ± 0.333

6.295 ± 0.352

6.007 ± 0.265

1.437 ± 0.143

4.116 ± 0.329
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski