Escherichia phage mckay

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dhillonvirus; unclassified Dhillonvirus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9X3I4|A0A6B9X3I4_9CAUD Calcineurin-like phosphoesterase superfamily domain protein OS=Escherichia phage mckay OX=2696416 GN=mckay_49 PE=4 SV=1
MM1 pKa = 7.29YY2 pKa = 10.23GDD4 pKa = 3.94PQTFIDD10 pKa = 3.83YY11 pKa = 10.12AAARR15 pKa = 11.84GVEE18 pKa = 4.25VTLIDD23 pKa = 3.6ATRR26 pKa = 11.84HH27 pKa = 4.17LTVVNDD33 pKa = 3.82FLNGINWIGEE43 pKa = 4.12PADD46 pKa = 3.53QTGIDD51 pKa = 3.16AWPRR55 pKa = 11.84INYY58 pKa = 9.13PSDD61 pKa = 3.2GKK63 pKa = 9.61PVRR66 pKa = 11.84DD67 pKa = 3.52TLTEE71 pKa = 3.88VLAVVPAGQIVDD83 pKa = 4.52FASIPVAVEE92 pKa = 3.33QAVYY96 pKa = 10.39RR97 pKa = 11.84LAMLVADD104 pKa = 4.92EE105 pKa = 4.63VDD107 pKa = 3.17ISPVGSGKK115 pKa = 7.93EE116 pKa = 4.14TIRR119 pKa = 11.84EE120 pKa = 4.16TVGPITMEE128 pKa = 3.9YY129 pKa = 10.31DD130 pKa = 3.28PASIGSGVSFPWWGGLLGHH149 pKa = 6.83WVDD152 pKa = 4.19SGGNAAGNFDD162 pKa = 3.97VFRR165 pKa = 11.84GG166 pKa = 3.62

Molecular weight: 17.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9XCX4|A0A6B9XCX4_9CAUD Uncharacterized protein OS=Escherichia phage mckay OX=2696416 GN=mckay_55 PE=4 SV=1
MM1 pKa = 7.2SASIRR6 pKa = 11.84DD7 pKa = 3.7MLMHH11 pKa = 6.54NRR13 pKa = 11.84FEE15 pKa = 4.11IGGRR19 pKa = 11.84VFRR22 pKa = 11.84NYY24 pKa = 9.44FVARR28 pKa = 11.84AWARR32 pKa = 11.84HH33 pKa = 4.64IGKK36 pKa = 9.03PEE38 pKa = 3.66GAIRR42 pKa = 11.84CYY44 pKa = 10.59TMM46 pKa = 4.87

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13559

39

1139

218.7

24.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.318 ± 0.539

1.232 ± 0.188

6.453 ± 0.333

6.129 ± 0.279

3.665 ± 0.151

7.714 ± 0.286

1.836 ± 0.178

5.391 ± 0.253

6.151 ± 0.341

7.493 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.184

4.226 ± 0.228

4.005 ± 0.243

3.798 ± 0.244

6.158 ± 0.281

4.809 ± 0.243

5.974 ± 0.275

7.242 ± 0.3

1.63 ± 0.153

3.002 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski