Flavobacteriaceae bacterium R33

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4113 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L9E6X2|A0A6L9E6X2_9FLAO Rrf2 family transcriptional regulator OS=Flavobacteriaceae bacterium R33 OX=2697053 GN=GTQ38_00685 PE=4 SV=1
MM1 pKa = 7.71LFLCLWITSDD11 pKa = 5.72LFAQSEE17 pKa = 5.26FITTWDD23 pKa = 3.35TTKK26 pKa = 10.52PGQSNDD32 pKa = 3.09NSITIPAYY40 pKa = 8.84GTYY43 pKa = 10.54DD44 pKa = 3.14VDD46 pKa = 4.17LGNDD50 pKa = 3.36GSYY53 pKa = 11.09EE54 pKa = 4.09LLSQTGTITVDD65 pKa = 3.39VTTHH69 pKa = 6.47NYY71 pKa = 7.39TAGLIQLALRR81 pKa = 11.84NAVSGNRR88 pKa = 11.84LTRR91 pKa = 11.84IHH93 pKa = 6.75FNYY96 pKa = 10.66AGDD99 pKa = 3.85RR100 pKa = 11.84QKK102 pKa = 11.35LLSVDD107 pKa = 3.1QWGSGIYY114 pKa = 9.2WSTMEE119 pKa = 5.07DD120 pKa = 3.28AFSGCTNLEE129 pKa = 3.96VKK131 pKa = 9.67ATDD134 pKa = 3.86FPNLGSVSNMSWMFGGCTSLTGATDD159 pKa = 4.08FSKK162 pKa = 10.65WDD164 pKa = 3.53TGKK167 pKa = 8.68VTDD170 pKa = 4.16MSFMFHH176 pKa = 6.77GAGAFNGNIASWNTDD191 pKa = 2.28KK192 pKa = 10.51VTNMQALFMDD202 pKa = 4.15TSAFNQNIASWNTSNVTTMANMFRR226 pKa = 11.84NAAVFDD232 pKa = 3.89QDD234 pKa = 3.08IGSWDD239 pKa = 3.64TGSVTDD245 pKa = 4.17MVSMFNSASNFDD257 pKa = 3.65QDD259 pKa = 5.28LGDD262 pKa = 3.55WDD264 pKa = 4.85LGKK267 pKa = 10.71LSNGTTMFKK276 pKa = 10.67SSGLSVGNWDD286 pKa = 3.22ATLIGWYY293 pKa = 7.15TQSFTNTPTIGASGLVYY310 pKa = 9.49CTAGTQRR317 pKa = 11.84AALTLNITGDD327 pKa = 3.8SAEE330 pKa = 4.4TTPPTAACQTAVTLQLGTSGTVMLDD355 pKa = 3.0AASVDD360 pKa = 3.54NGSSDD365 pKa = 3.27TCGNVSLGLSQSSFTGSDD383 pKa = 2.8LGTNTVTLTVTDD395 pKa = 4.07PNGNKK400 pKa = 9.67NSCTIVVTVEE410 pKa = 4.26DD411 pKa = 4.92KK412 pKa = 10.78IAPTAACKK420 pKa = 8.76DD421 pKa = 3.74TKK423 pKa = 11.1VEE425 pKa = 3.98LDD427 pKa = 3.53SAGSATITAANVNNNSSDD445 pKa = 3.38NSGSVILSLNTSGYY459 pKa = 9.94DD460 pKa = 3.39CSKK463 pKa = 9.66TGEE466 pKa = 4.19NTVTLTVSDD475 pKa = 4.13GSGNSDD481 pKa = 3.12SCTAKK486 pKa = 9.47VTVEE490 pKa = 4.4DD491 pKa = 4.14KK492 pKa = 10.72LAPSATDD499 pKa = 3.87LAPMNLQCSSVAPAADD515 pKa = 3.06ISIVKK520 pKa = 10.18DD521 pKa = 3.32VTDD524 pKa = 3.66NCTANPTVAFVSDD537 pKa = 3.76VSDD540 pKa = 3.89GNSNPEE546 pKa = 4.24VITRR550 pKa = 11.84TYY552 pKa = 11.1SVTDD556 pKa = 3.51GAGNSTTLTQKK567 pKa = 8.8ITLGDD572 pKa = 3.63TTAPTANMLSPISVEE587 pKa = 4.19CKK589 pKa = 10.32SNVPAADD596 pKa = 3.18IALVTGVADD605 pKa = 3.44NCTANPTVAFVSDD618 pKa = 3.76VSDD621 pKa = 3.89GNSNPEE627 pKa = 4.24VITRR631 pKa = 11.84TYY633 pKa = 11.1SVTDD637 pKa = 3.51GAGNSTPLTQTITVNDD653 pKa = 3.83TTAPTVLCQSVTLQLGTDD671 pKa = 4.08GTASLTANLVDD682 pKa = 3.99NGSNDD687 pKa = 3.08ACGSVTLNLSSYY699 pKa = 11.42SFTTSDD705 pKa = 4.15LGDD708 pKa = 3.44NTVTLTVTDD717 pKa = 4.41PNGNTNTCQATVTIVDD733 pKa = 3.85PASVFVTTWDD743 pKa = 3.3TTKK746 pKa = 10.65PGTSDD751 pKa = 3.22GKK753 pKa = 11.04SITIPVSGTYY763 pKa = 10.4DD764 pKa = 3.04VDD766 pKa = 3.92LGNDD770 pKa = 3.55GTYY773 pKa = 11.09EE774 pKa = 4.28LLDD777 pKa = 3.61QNGTLTLDD785 pKa = 3.24VTTYY789 pKa = 10.42KK790 pKa = 9.35HH791 pKa = 5.21TAGEE795 pKa = 3.97IQLALRR801 pKa = 11.84NAASGNGSLSRR812 pKa = 11.84IHH814 pKa = 6.58FNNSGDD820 pKa = 3.79RR821 pKa = 11.84QKK823 pKa = 11.37LLSVDD828 pKa = 3.12QWGSSITWSTMEE840 pKa = 3.86EE841 pKa = 3.99AFYY844 pKa = 11.13GCANLEE850 pKa = 4.32VKK852 pKa = 9.63ATDD855 pKa = 3.95APNLSYY861 pKa = 9.88VTTMIEE867 pKa = 4.23SFMLCTSLKK876 pKa = 9.1GTTSFPSWNTTGVTDD891 pKa = 3.48MSYY894 pKa = 9.81MFNQASSFNGNISSWEE910 pKa = 4.11TGNVTNMAAMFTGASSFDD928 pKa = 3.86RR929 pKa = 11.84NIGSWNTSGVTNMASMFYY947 pKa = 9.9QASSFNANIGSWNTSNVTTMVSMFYY972 pKa = 9.9QASSFDD978 pKa = 3.88EE979 pKa = 5.59DD980 pKa = 4.25LGNWDD985 pKa = 4.46IGQLTNGTGMLNGSGLSMEE1004 pKa = 4.54NWDD1007 pKa = 3.69DD1008 pKa = 3.9TLIGWHH1014 pKa = 5.64SQGFTNAVTIGASGLHH1030 pKa = 5.55YY1031 pKa = 10.19CQAGAEE1037 pKa = 4.07RR1038 pKa = 11.84AAMTLSFSGDD1048 pKa = 3.72SVEE1051 pKa = 4.53DD1052 pKa = 3.7TAPTASNPASVSVKK1066 pKa = 10.23CSSDD1070 pKa = 3.31VPAADD1075 pKa = 2.91IAVVTDD1081 pKa = 3.68EE1082 pKa = 5.16SDD1084 pKa = 3.2NCTASPTVTFVSDD1097 pKa = 3.62VSDD1100 pKa = 3.92GNSNPEE1106 pKa = 4.23VITRR1110 pKa = 11.84TYY1112 pKa = 10.63RR1113 pKa = 11.84ITDD1116 pKa = 3.34EE1117 pKa = 5.0AGNSTDD1123 pKa = 3.14VTQTITIEE1131 pKa = 4.12DD1132 pKa = 3.74TT1133 pKa = 3.49

Molecular weight: 118.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L9EHV2|A0A6L9EHV2_9FLAO MMPL family transporter OS=Flavobacteriaceae bacterium R33 OX=2697053 GN=GTQ38_19655 PE=4 SV=1
MM1 pKa = 7.74PGYY4 pKa = 9.93IKK6 pKa = 10.67NRR8 pKa = 11.84IKK10 pKa = 11.03GRR12 pKa = 11.84GFPWFRR18 pKa = 11.84AVGMICLFPIRR29 pKa = 11.84HH30 pKa = 6.45KK31 pKa = 10.84GAQCAWAGRR40 pKa = 11.84YY41 pKa = 8.33MDD43 pKa = 5.56DD44 pKa = 2.72MHH46 pKa = 9.12LLFRR50 pKa = 11.84VLKK53 pKa = 10.05RR54 pKa = 11.84SKK56 pKa = 9.58PLPSAWEE63 pKa = 3.87VLGG66 pKa = 4.16

Molecular weight: 7.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4113

0

4113

1474473

32

8468

358.5

40.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.943 ± 0.037

0.766 ± 0.016

5.927 ± 0.048

6.8 ± 0.06

5.036 ± 0.036

7.095 ± 0.044

1.798 ± 0.021

6.905 ± 0.04

6.326 ± 0.076

9.66 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.021

5.243 ± 0.047

3.75 ± 0.032

3.509 ± 0.023

4.236 ± 0.04

6.704 ± 0.052

5.693 ± 0.103

6.293 ± 0.032

1.227 ± 0.017

3.865 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski