Lactococcus phage Q54

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Questintvirus; Lactococcus virus Q54

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0GXU2|Q0GXU2_9CAUD Minor structural protein OS=Lactococcus phage Q54 OX=382685 PE=4 SV=1
MM1 pKa = 7.59SYY3 pKa = 10.82KK4 pKa = 10.14IFKK7 pKa = 9.0STTASMYY14 pKa = 10.62DD15 pKa = 3.26VSSSSNIDD23 pKa = 3.23IKK25 pKa = 10.69PTGLDD30 pKa = 3.91FIVLSISDD38 pKa = 3.73HH39 pKa = 6.55AEE41 pKa = 3.77PGEE44 pKa = 4.45DD45 pKa = 3.45FTITLSEE52 pKa = 4.16NEE54 pKa = 4.17PLLTYY59 pKa = 10.76LNQLYY64 pKa = 9.16TIRR67 pKa = 11.84DD68 pKa = 3.63NFNEE72 pKa = 3.58NSYY75 pKa = 11.12NITPFFVTGSDD86 pKa = 3.37RR87 pKa = 11.84EE88 pKa = 4.25FGKK91 pKa = 8.33ITYY94 pKa = 7.72TCTKK98 pKa = 9.28VYY100 pKa = 10.09KK101 pKa = 10.38GYY103 pKa = 10.57LIVPGIFEE111 pKa = 4.44GEE113 pKa = 4.0ILL115 pKa = 4.22

Molecular weight: 13.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0GXT7|Q0GXT7_9CAUD Uncharacterized protein OS=Lactococcus phage Q54 OX=382685 PE=4 SV=1
MM1 pKa = 7.44SKK3 pKa = 10.98DD4 pKa = 3.06FFKK7 pKa = 10.78RR8 pKa = 11.84FKK10 pKa = 10.92YY11 pKa = 9.77KK12 pKa = 10.85GKK14 pKa = 10.33IGGAFNSEE22 pKa = 4.22LRR24 pKa = 11.84SGVYY28 pKa = 8.95VKK30 pKa = 10.69KK31 pKa = 10.76RR32 pKa = 11.84GDD34 pKa = 3.38GQYY37 pKa = 10.92KK38 pKa = 9.83IGVNINSSQMAILDD52 pKa = 3.45SRR54 pKa = 11.84GWVLRR59 pKa = 11.84RR60 pKa = 11.84GKK62 pKa = 9.14QNKK65 pKa = 9.4KK66 pKa = 9.96GLLVHH71 pKa = 6.97GATKK75 pKa = 10.03EE76 pKa = 3.91QNFDD80 pKa = 3.31FNAAEE85 pKa = 4.19RR86 pKa = 11.84KK87 pKa = 7.71VQKK90 pKa = 10.78ARR92 pKa = 11.84DD93 pKa = 3.81ATQSVIDD100 pKa = 4.53SVPQQ104 pKa = 3.37

Molecular weight: 11.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

8051

37

769

171.3

19.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.44 ± 0.601

0.373 ± 0.118

5.614 ± 0.207

7.465 ± 0.399

4.385 ± 0.296

6.533 ± 0.341

1.255 ± 0.193

5.664 ± 0.244

8.868 ± 0.536

8.831 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.633 ± 0.131

5.999 ± 0.366

2.832 ± 0.262

3.503 ± 0.34

3.9 ± 0.281

5.614 ± 0.344

7.105 ± 0.349

6.434 ± 0.279

1.043 ± 0.129

4.509 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski