Blotched snakehead virus (BSNV) (Channa lucius virus)

Taxonomy: Viruses; Riboviria; Orthornavirae; Birnaviridae; Blosnavirus

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q8AZM0|POLS_BSNV Structural polyprotein OS=Blotched snakehead virus OX=311176 PE=1 SV=1
MM1 pKa = 7.97DD2 pKa = 4.58FSKK5 pKa = 11.13EE6 pKa = 3.92NTQIRR11 pKa = 11.84YY12 pKa = 9.54LNSLLVPEE20 pKa = 5.0TGSTSIPDD28 pKa = 3.52DD29 pKa = 3.54TLDD32 pKa = 3.49RR33 pKa = 11.84HH34 pKa = 5.58CLKK37 pKa = 10.25TEE39 pKa = 3.93TTTEE43 pKa = 3.98NLVAALGGSGLIVLFPNSPSGLLGAHH69 pKa = 5.23YY70 pKa = 8.57TKK72 pKa = 10.3TPQGSLIFDD81 pKa = 3.8KK82 pKa = 11.25AITTSQDD89 pKa = 2.56LKK91 pKa = 10.71KK92 pKa = 10.38AYY94 pKa = 10.34NYY96 pKa = 10.35ARR98 pKa = 11.84LVSRR102 pKa = 11.84IVQVRR107 pKa = 11.84SSTLPAGVYY116 pKa = 10.3ALNGTFNGVTYY127 pKa = 10.33IGSLSEE133 pKa = 5.5IKK135 pKa = 10.78DD136 pKa = 3.53LDD138 pKa = 4.14YY139 pKa = 11.78NSLLSATANINDD151 pKa = 3.56KK152 pKa = 10.83VGNVLVGDD160 pKa = 4.49GVAVLSLPAGSDD172 pKa = 3.37LPYY175 pKa = 10.7VRR177 pKa = 11.84LGDD180 pKa = 4.15EE181 pKa = 4.63VPSSAGVARR190 pKa = 11.84CSPSDD195 pKa = 3.44RR196 pKa = 11.84PRR198 pKa = 11.84HH199 pKa = 4.92YY200 pKa = 10.67NANNKK205 pKa = 7.72QVQVGTTDD213 pKa = 2.94TKK215 pKa = 11.11TNGFNIDD222 pKa = 3.12ATTPTEE228 pKa = 4.12VTVDD232 pKa = 3.41MQIAQIAAGKK242 pKa = 7.69TLTVTVKK249 pKa = 10.8LMGLTGAKK257 pKa = 9.11VASRR261 pKa = 11.84SEE263 pKa = 4.38TVSGNGGTFHH273 pKa = 7.61FSTTAVFGEE282 pKa = 4.5TEE284 pKa = 3.6ITQPVVGVQVLAKK297 pKa = 9.84TNGDD301 pKa = 4.14PIVVDD306 pKa = 3.51SYY308 pKa = 11.87VGVTVHH314 pKa = 6.55GGNMPGTLRR323 pKa = 11.84PVTIIAYY330 pKa = 9.09EE331 pKa = 4.26SVATGSVLTLSGISNYY347 pKa = 8.83EE348 pKa = 4.17LIPNPEE354 pKa = 4.06LAKK357 pKa = 10.68NIQTSYY363 pKa = 11.25GKK365 pKa = 10.58LNPAEE370 pKa = 3.92MTYY373 pKa = 10.14TKK375 pKa = 10.69VVLSHH380 pKa = 7.01RR381 pKa = 11.84DD382 pKa = 3.52EE383 pKa = 5.0LGLRR387 pKa = 11.84SIWSIPQYY395 pKa = 10.64RR396 pKa = 11.84DD397 pKa = 2.79MMSYY401 pKa = 10.06FRR403 pKa = 11.84EE404 pKa = 4.02VSDD407 pKa = 3.74RR408 pKa = 11.84SSPLKK413 pKa = 10.33IAGAFGWGDD422 pKa = 3.76LLSGIRR428 pKa = 11.84KK429 pKa = 8.19WVFPVVDD436 pKa = 3.95TLLPAARR443 pKa = 11.84PLTDD447 pKa = 5.16LASGWIKK454 pKa = 10.94NKK456 pKa = 10.24YY457 pKa = 8.75PEE459 pKa = 4.45AASGRR464 pKa = 11.84PLAASGRR471 pKa = 11.84PMAASGTFSKK481 pKa = 10.64RR482 pKa = 11.84IPLASSDD489 pKa = 3.95EE490 pKa = 3.88IDD492 pKa = 4.02YY493 pKa = 11.33QSVLALTIPGTHH505 pKa = 7.19PKK507 pKa = 9.98LVPPTEE513 pKa = 4.33RR514 pKa = 11.84EE515 pKa = 4.17PNSTPDD521 pKa = 2.95GHH523 pKa = 6.98KK524 pKa = 9.43ITGAKK529 pKa = 9.08TKK531 pKa = 10.59DD532 pKa = 3.32NTGGDD537 pKa = 3.71VTVVKK542 pKa = 10.36PLDD545 pKa = 3.49WLFKK549 pKa = 10.43LPCLRR554 pKa = 11.84PQAADD559 pKa = 3.71LPISLLQTLAYY570 pKa = 8.74KK571 pKa = 10.46QPLGRR576 pKa = 11.84NSRR579 pKa = 11.84IVHH582 pKa = 5.47FTDD585 pKa = 4.02GALFPVVAFGDD596 pKa = 3.53NHH598 pKa = 5.9STSEE602 pKa = 4.03LYY604 pKa = 9.93IAVRR608 pKa = 11.84GDD610 pKa = 3.33HH611 pKa = 7.29RR612 pKa = 11.84DD613 pKa = 3.32LMSPDD618 pKa = 3.34VRR620 pKa = 11.84DD621 pKa = 3.83SYY623 pKa = 12.17ALTGDD628 pKa = 3.69DD629 pKa = 3.76HH630 pKa = 6.89KK631 pKa = 11.7VWGATHH637 pKa = 5.52HH638 pKa = 6.08TYY640 pKa = 10.59YY641 pKa = 10.99VEE643 pKa = 4.44GAPKK647 pKa = 10.27KK648 pKa = 9.48PLKK651 pKa = 10.76FNVKK655 pKa = 8.37TRR657 pKa = 11.84TDD659 pKa = 3.54LTILPVADD667 pKa = 3.74VFWRR671 pKa = 11.84ADD673 pKa = 3.02GSADD677 pKa = 4.27VDD679 pKa = 4.38VVWNDD684 pKa = 3.21MPAVAGQSSSIALALASSLPFVPKK708 pKa = 10.33AAYY711 pKa = 7.66TGCLSGTNVQPVQFGNLKK729 pKa = 10.06ARR731 pKa = 11.84AAHH734 pKa = 6.96KK735 pKa = 10.35IGLPLVGMTQDD746 pKa = 3.14GGEE749 pKa = 4.19DD750 pKa = 3.48TRR752 pKa = 11.84ICTLDD757 pKa = 3.71DD758 pKa = 4.25AADD761 pKa = 3.59HH762 pKa = 6.81AFDD765 pKa = 4.33SMEE768 pKa = 4.18STVTRR773 pKa = 11.84PEE775 pKa = 4.34SVGHH779 pKa = 5.22QAAFQGWFYY788 pKa = 11.13CGAADD793 pKa = 4.36EE794 pKa = 4.42EE795 pKa = 4.87TIEE798 pKa = 4.11EE799 pKa = 4.7LEE801 pKa = 4.74DD802 pKa = 3.82FLDD805 pKa = 4.9SIEE808 pKa = 4.36LHH810 pKa = 6.33SKK812 pKa = 8.3PTVEE816 pKa = 4.44QPQTEE821 pKa = 4.24EE822 pKa = 3.9AMEE825 pKa = 4.3LLMEE829 pKa = 5.13LARR832 pKa = 11.84KK833 pKa = 9.16DD834 pKa = 3.55PQMSKK839 pKa = 10.25ILVILGWVEE848 pKa = 3.82GAGLIDD854 pKa = 5.41ALYY857 pKa = 10.93NWAQLDD863 pKa = 3.84DD864 pKa = 4.18GGVRR868 pKa = 11.84MRR870 pKa = 11.84NMLRR874 pKa = 11.84NLPHH878 pKa = 6.97EE879 pKa = 4.72GSKK882 pKa = 9.04SQRR885 pKa = 11.84RR886 pKa = 11.84KK887 pKa = 10.05HH888 pKa = 5.77GPAPEE893 pKa = 4.24SRR895 pKa = 11.84EE896 pKa = 4.06STRR899 pKa = 11.84MEE901 pKa = 3.99VLRR904 pKa = 11.84RR905 pKa = 11.84EE906 pKa = 4.15AAAKK910 pKa = 9.78RR911 pKa = 11.84KK912 pKa = 8.53KK913 pKa = 10.01AQRR916 pKa = 11.84ISEE919 pKa = 4.16DD920 pKa = 3.19AMDD923 pKa = 4.34NGFEE927 pKa = 4.16FATIDD932 pKa = 3.22WVLEE936 pKa = 4.04NGSRR940 pKa = 11.84GPNPAQAKK948 pKa = 8.52YY949 pKa = 10.62YY950 pKa = 10.15KK951 pKa = 9.92ATGLDD956 pKa = 3.86PEE958 pKa = 5.19PGLTEE963 pKa = 4.17FLPEE967 pKa = 3.89PTHH970 pKa = 7.63APEE973 pKa = 4.03NKK975 pKa = 9.32AAKK978 pKa = 9.59LAATIYY984 pKa = 10.23GSPNQAPAPPEE995 pKa = 3.79FVEE998 pKa = 4.67EE999 pKa = 4.15VAAVLMEE1006 pKa = 4.37NNGRR1010 pKa = 11.84GPNQAQMRR1018 pKa = 11.84EE1019 pKa = 4.17LRR1021 pKa = 11.84LKK1023 pKa = 10.66ALTMKK1028 pKa = 10.32SGSGAAATFKK1038 pKa = 10.5PRR1040 pKa = 11.84NRR1042 pKa = 11.84RR1043 pKa = 11.84PAQEE1047 pKa = 3.99YY1048 pKa = 9.21QPRR1051 pKa = 11.84PPITSRR1057 pKa = 11.84AGRR1060 pKa = 11.84FLNISTTLSS1069 pKa = 3.19

Molecular weight: 115.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q8AZM0|POLS_BSNV Structural polyprotein OS=Blotched snakehead virus OX=311176 PE=1 SV=1
MM1 pKa = 8.07PDD3 pKa = 3.86LSAALCRR10 pKa = 11.84CGAQRR15 pKa = 11.84SPRR18 pKa = 11.84VSTHH22 pKa = 4.16STGPSMGLPTLEE34 pKa = 3.64VSARR38 pKa = 11.84SRR40 pKa = 11.84TWTTIHH46 pKa = 7.04FSLQRR51 pKa = 11.84PTSMTRR57 pKa = 11.84SAMYY61 pKa = 10.69LLEE64 pKa = 4.3TVSQCSHH71 pKa = 6.83FLLEE75 pKa = 4.55ATCLMLDD82 pKa = 4.34LAMKK86 pKa = 9.51FQALLEE92 pKa = 4.47SRR94 pKa = 11.84DD95 pKa = 3.93ALPPTDD101 pKa = 5.49LGTTMPTTNRR111 pKa = 11.84SRR113 pKa = 11.84SARR116 pKa = 11.84PTPRR120 pKa = 11.84PMGSTSTQQHH130 pKa = 5.35PQRR133 pKa = 11.84SRR135 pKa = 11.84STCRR139 pKa = 3.04

Molecular weight: 15.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2075

139

1069

691.7

75.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.578 ± 0.598

0.867 ± 0.631

5.205 ± 0.604

5.735 ± 0.837

2.795 ± 0.148

7.181 ± 0.915

1.976 ± 0.21

3.904 ± 0.733

5.301 ± 1.164

9.976 ± 0.531

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.747 ± 0.758

4.53 ± 0.957

6.699 ± 0.477

3.422 ± 0.38

5.446 ± 1.228

7.47 ± 1.454

8.241 ± 1.246

5.735 ± 1.144

1.446 ± 0.297

2.747 ± 0.469
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski