Circoviridae 13 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples

Average proteome isoelectric point is 7.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5T695|S5T695_9CIRC Uncharacterized protein OS=Circoviridae 13 LDMD-2013 OX=1379717 PE=4 SV=1
MM1 pKa = 7.34SKK3 pKa = 10.28YY4 pKa = 9.49WCFTLNNYY12 pKa = 7.41TRR14 pKa = 11.84EE15 pKa = 3.94EE16 pKa = 4.14EE17 pKa = 4.2EE18 pKa = 4.06AVKK21 pKa = 10.39ALCEE25 pKa = 4.27GEE27 pKa = 5.0SPIADD32 pKa = 3.34YY33 pKa = 10.94LVYY36 pKa = 10.53GRR38 pKa = 11.84EE39 pKa = 3.93RR40 pKa = 11.84GEE42 pKa = 4.9GEE44 pKa = 4.42DD45 pKa = 3.84TPHH48 pKa = 5.89LQGYY52 pKa = 9.64IEE54 pKa = 4.21FKK56 pKa = 9.34NRR58 pKa = 11.84KK59 pKa = 8.69RR60 pKa = 11.84FSTVRR65 pKa = 11.84TVIPRR70 pKa = 11.84AHH72 pKa = 6.9IEE74 pKa = 3.78RR75 pKa = 11.84RR76 pKa = 11.84MGSASEE82 pKa = 3.6AAAYY86 pKa = 8.56CKK88 pKa = 10.06KK89 pKa = 10.83DD90 pKa = 3.07EE91 pKa = 4.85DD92 pKa = 4.19FFEE95 pKa = 5.81HH96 pKa = 6.74GVISQGQGARR106 pKa = 11.84NDD108 pKa = 3.61LARR111 pKa = 11.84LHH113 pKa = 6.1EE114 pKa = 5.16AIFVEE119 pKa = 4.76QKK121 pKa = 10.54SVAHH125 pKa = 5.12VQEE128 pKa = 5.04EE129 pKa = 5.12YY130 pKa = 10.01FGSYY134 pKa = 8.96IKK136 pKa = 9.34YY137 pKa = 9.28HH138 pKa = 6.31RR139 pKa = 11.84GIEE142 pKa = 4.09KK143 pKa = 10.4VLQQRR148 pKa = 11.84AKK150 pKa = 9.54PRR152 pKa = 11.84TWKK155 pKa = 10.63SRR157 pKa = 11.84VVVYY161 pKa = 8.91WGPTGTGKK169 pKa = 7.96TRR171 pKa = 11.84RR172 pKa = 11.84CFASSDD178 pKa = 3.78PPTWIYY184 pKa = 11.35SSDD187 pKa = 3.03GWFDD191 pKa = 3.99GYY193 pKa = 11.25QGDD196 pKa = 4.09EE197 pKa = 4.15TVLFDD202 pKa = 5.35DD203 pKa = 4.97FGGHH207 pKa = 5.76EE208 pKa = 4.34FRR210 pKa = 11.84LTHH213 pKa = 6.75LLKK216 pKa = 11.12LLDD219 pKa = 4.45RR220 pKa = 11.84YY221 pKa = 10.07PMRR224 pKa = 11.84VKK226 pKa = 10.8VKK228 pKa = 10.06GSFVQWCPKK237 pKa = 9.3TIYY240 pKa = 8.68ITSNIEE246 pKa = 3.47PAFWYY251 pKa = 9.37PNARR255 pKa = 11.84EE256 pKa = 4.05VHH258 pKa = 6.27RR259 pKa = 11.84EE260 pKa = 3.43ALMRR264 pKa = 11.84RR265 pKa = 11.84IDD267 pKa = 5.09DD268 pKa = 3.71IEE270 pKa = 4.07EE271 pKa = 4.17MADD274 pKa = 3.04EE275 pKa = 4.47WVPPVPVEE283 pKa = 4.31NEE285 pKa = 4.23VIDD288 pKa = 4.19LTDD291 pKa = 3.22EE292 pKa = 4.05

Molecular weight: 34.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5T695|S5T695_9CIRC Uncharacterized protein OS=Circoviridae 13 LDMD-2013 OX=1379717 PE=4 SV=1
MM1 pKa = 8.16PDD3 pKa = 3.15LDD5 pKa = 4.31QNFTYY10 pKa = 10.47SPPLPTFIGPCQVGPRR26 pKa = 11.84EE27 pKa = 3.97RR28 pKa = 11.84AVLGVSRR35 pKa = 11.84RR36 pKa = 11.84TCDD39 pKa = 2.9EE40 pKa = 3.82VAFRR44 pKa = 11.84DD45 pKa = 3.61YY46 pKa = 11.05FQIFRR51 pKa = 11.84AIYY54 pKa = 9.41IMLPKK59 pKa = 10.07RR60 pKa = 11.84IQQIKK65 pKa = 8.9PARR68 pKa = 11.84ANQGMLRR75 pKa = 11.84DD76 pKa = 3.65HH77 pKa = 6.53MRR79 pKa = 11.84AFMQTHH85 pKa = 6.95RR86 pKa = 11.84GSKK89 pKa = 10.03ARR91 pKa = 11.84IIAEE95 pKa = 4.05LASLGWHH102 pKa = 5.73ITPSFVKK109 pKa = 10.69GLGLSFGTWSATKK122 pKa = 10.48KK123 pKa = 10.17FIKK126 pKa = 10.3KK127 pKa = 9.84FEE129 pKa = 4.36KK130 pKa = 10.1PYY132 pKa = 10.34EE133 pKa = 4.14WDD135 pKa = 3.5DD136 pKa = 3.54APPRR140 pKa = 11.84VEE142 pKa = 4.6KK143 pKa = 10.69RR144 pKa = 11.84KK145 pKa = 9.08QSKK148 pKa = 10.07IKK150 pKa = 10.57FPSKK154 pKa = 8.76KK155 pKa = 9.35QKK157 pKa = 9.78RR158 pKa = 11.84VHH160 pKa = 6.32PTTQLLADD168 pKa = 3.79VAFGASKK175 pKa = 10.8KK176 pKa = 10.3SLSKK180 pKa = 9.79STYY183 pKa = 7.38TKK185 pKa = 10.29QMPYY189 pKa = 9.92RR190 pKa = 11.84RR191 pKa = 11.84KK192 pKa = 9.95GYY194 pKa = 10.18GKK196 pKa = 10.3SGMQKK201 pKa = 9.3RR202 pKa = 11.84RR203 pKa = 11.84RR204 pKa = 11.84YY205 pKa = 8.64KK206 pKa = 9.93KK207 pKa = 9.05KK208 pKa = 7.97RR209 pKa = 11.84QRR211 pKa = 11.84RR212 pKa = 11.84SRR214 pKa = 11.84SFGLTKK220 pKa = 10.3KK221 pKa = 10.14IKK223 pKa = 9.68RR224 pKa = 11.84QIRR227 pKa = 11.84NIAKK231 pKa = 9.98RR232 pKa = 11.84ISHH235 pKa = 6.62EE236 pKa = 4.25SLPQEE241 pKa = 3.75TRR243 pKa = 11.84EE244 pKa = 4.14IDD246 pKa = 3.07NHH248 pKa = 6.03QYY250 pKa = 10.92SSPVNTASYY259 pKa = 10.85NAIDD263 pKa = 4.32CLTRR267 pKa = 11.84DD268 pKa = 4.91DD269 pKa = 5.18VDD271 pKa = 3.45NLLNYY276 pKa = 7.8TYY278 pKa = 8.58PTIDD282 pKa = 4.05GGTIDD287 pKa = 4.24WKK289 pKa = 11.04DD290 pKa = 3.57LSSTNSDD297 pKa = 3.0CRR299 pKa = 11.84VYY301 pKa = 9.5FTKK304 pKa = 10.71GSYY307 pKa = 9.07LQLWIRR313 pKa = 11.84NNFDD317 pKa = 3.45YY318 pKa = 10.63PVTMKK323 pKa = 10.14FYY325 pKa = 10.08PYY327 pKa = 10.49KK328 pKa = 10.7CADD331 pKa = 3.59STNVDD336 pKa = 3.87AEE338 pKa = 4.41TEE340 pKa = 4.21MTNKK344 pKa = 9.63FQEE347 pKa = 4.31TQYY350 pKa = 11.57SVAAPPLILGNAKK363 pKa = 9.57QYY365 pKa = 11.39YY366 pKa = 9.21FINPSDD372 pKa = 3.77VKK374 pKa = 9.85MKK376 pKa = 10.06DD377 pKa = 2.93WKK379 pKa = 10.72RR380 pKa = 11.84HH381 pKa = 4.15GKK383 pKa = 9.41AQTKK387 pKa = 9.75IINPSEE393 pKa = 4.04EE394 pKa = 4.41CKK396 pKa = 10.93VSFPLTGYY404 pKa = 10.29YY405 pKa = 10.28DD406 pKa = 3.51YY407 pKa = 11.62RR408 pKa = 11.84NANDD412 pKa = 3.1QGTTYY417 pKa = 10.66IAGCSHH423 pKa = 6.75SLLIKK428 pKa = 10.29QYY430 pKa = 10.12GHH432 pKa = 7.34PSHH435 pKa = 6.74GKK437 pKa = 9.58QEE439 pKa = 3.84VDD441 pKa = 3.14AQGLNTGLSDD451 pKa = 3.5TKK453 pKa = 10.56VDD455 pKa = 4.02VISRR459 pKa = 11.84CNLKK463 pKa = 10.7YY464 pKa = 10.76KK465 pKa = 10.64LVTKK469 pKa = 10.47DD470 pKa = 3.34EE471 pKa = 4.47IGHH474 pKa = 6.2IKK476 pKa = 10.17HH477 pKa = 5.83GQNLDD482 pKa = 3.27RR483 pKa = 11.84YY484 pKa = 9.04GG485 pKa = 3.31

Molecular weight: 55.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

777

292

485

388.5

45.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.663 ± 0.248

1.673 ± 0.019

5.92 ± 0.049

5.92 ± 2.151

4.247 ± 0.271

6.306 ± 0.268

2.703 ± 0.188

5.663 ± 0.26

8.366 ± 1.426

5.792 ± 0.323

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.931 ± 0.108

3.604 ± 0.596

5.148 ± 0.175

4.505 ± 0.703

7.593 ± 0.309

6.435 ± 0.641

6.049 ± 0.451

5.277 ± 0.946

1.802 ± 0.463

5.405 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski