Hubei leech virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KP21|A0A1L3KP21_9VIRU Putative capsid protein OS=Hubei leech virus 1 OX=1922899 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 6.15LGNSPGWPWRR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 4.14VRR16 pKa = 11.84TKK18 pKa = 10.39QQAIDD23 pKa = 3.79AFPEE27 pKa = 3.96VLTYY31 pKa = 9.96WDD33 pKa = 3.52APGPVVWHH41 pKa = 6.26GFGKK45 pKa = 10.87LEE47 pKa = 4.02ILPSEE52 pKa = 4.24KK53 pKa = 10.15ADD55 pKa = 3.81RR56 pKa = 11.84KK57 pKa = 10.33CRR59 pKa = 11.84LITGAPVDD67 pKa = 4.23LALLGARR74 pKa = 11.84LYY76 pKa = 11.28EE77 pKa = 4.32DD78 pKa = 3.54FNDD81 pKa = 4.41RR82 pKa = 11.84FTTLHH87 pKa = 6.31TEE89 pKa = 3.85THH91 pKa = 6.11SALGMSKK98 pKa = 8.94FHH100 pKa = 7.03RR101 pKa = 11.84GWATLAAKK109 pKa = 9.65MDD111 pKa = 4.08GPVTEE116 pKa = 5.57ADD118 pKa = 3.19AKK120 pKa = 10.63QWDD123 pKa = 3.58SSMTIKK129 pKa = 10.78LLEE132 pKa = 3.93AVYY135 pKa = 10.22RR136 pKa = 11.84IRR138 pKa = 11.84WACLRR143 pKa = 11.84SEE145 pKa = 4.57DD146 pKa = 3.71QTAGNWARR154 pKa = 11.84HH155 pKa = 4.13QAYY158 pKa = 10.05LRR160 pKa = 11.84EE161 pKa = 4.37LTHH164 pKa = 5.88STIHH168 pKa = 6.75LPDD171 pKa = 4.0GSMWEE176 pKa = 4.15AIGGNKK182 pKa = 9.6SGSVNTAHH190 pKa = 7.62DD191 pKa = 3.72NTIGHH196 pKa = 7.31IIVCLYY202 pKa = 10.31AWLRR206 pKa = 11.84LGRR209 pKa = 11.84CSKK212 pKa = 10.41EE213 pKa = 4.19RR214 pKa = 11.84FWEE217 pKa = 4.47YY218 pKa = 10.77PFALYY223 pKa = 10.81GDD225 pKa = 4.67DD226 pKa = 4.45FLAGKK231 pKa = 10.29LEE233 pKa = 3.7EE234 pKa = 4.59RR235 pKa = 11.84FFDD238 pKa = 4.71FYY240 pKa = 11.28RR241 pKa = 11.84EE242 pKa = 3.83TGIRR246 pKa = 11.84LPRR249 pKa = 11.84EE250 pKa = 3.61NVVQHH255 pKa = 6.48DD256 pKa = 4.02QLEE259 pKa = 4.71GASFLSQRR267 pKa = 11.84FKK269 pKa = 11.08KK270 pKa = 10.68VDD272 pKa = 3.86GQWVAEE278 pKa = 4.02PDD280 pKa = 3.41GAKK283 pKa = 10.59ALFSACTSDD292 pKa = 4.47LKK294 pKa = 11.17PNLRR298 pKa = 11.84LAFEE302 pKa = 4.57RR303 pKa = 11.84VRR305 pKa = 11.84SLWLDD310 pKa = 3.72AYY312 pKa = 8.09WTEE315 pKa = 4.31AEE317 pKa = 4.08EE318 pKa = 4.32TLRR321 pKa = 11.84RR322 pKa = 11.84ACNSLADD329 pKa = 3.92EE330 pKa = 4.66LGEE333 pKa = 4.0PRR335 pKa = 11.84VSRR338 pKa = 11.84LLAKK342 pKa = 10.2RR343 pKa = 11.84IWNCYY348 pKa = 7.86EE349 pKa = 3.62IEE351 pKa = 4.61GGSGVSASPQNGEE364 pKa = 4.33HH365 pKa = 6.22NFITAADD372 pKa = 3.47HH373 pKa = 7.07CEE375 pKa = 3.91RR376 pKa = 11.84EE377 pKa = 4.63SVGEE381 pKa = 3.89NLPPPANLL389 pKa = 3.64

Molecular weight: 44.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KNW4|A0A1L3KNW4_9VIRU RNA-dependent RNA polymerase OS=Hubei leech virus 1 OX=1922899 PE=4 SV=1
MM1 pKa = 7.55TYY3 pKa = 10.49WNIKK7 pKa = 10.04VIMTILLLGVAAVAVIAGVLFVCFKK32 pKa = 10.9VRR34 pKa = 11.84VSTQQAASLTSTFIGFCVFLWIVRR58 pKa = 11.84ALVRR62 pKa = 11.84RR63 pKa = 11.84YY64 pKa = 10.12GPNKK68 pKa = 8.3TVRR71 pKa = 11.84EE72 pKa = 3.96LVMEE76 pKa = 4.48RR77 pKa = 11.84FKK79 pKa = 11.2KK80 pKa = 10.2KK81 pKa = 9.71RR82 pKa = 11.84SKK84 pKa = 10.15AAKK87 pKa = 9.57GPSGTMAGDD96 pKa = 3.62QYY98 pKa = 11.34QVNLIPGVTDD108 pKa = 4.38LLSEE112 pKa = 4.2LWDD115 pKa = 4.28DD116 pKa = 4.21VDD118 pKa = 4.67DD119 pKa = 4.39TFTLAVPLFAMGGVAWGRR137 pKa = 11.84DD138 pKa = 3.3LLFIQTAIRR147 pKa = 11.84KK148 pKa = 8.12FVATLPGWLDD158 pKa = 3.6LPLGDD163 pKa = 4.68LRR165 pKa = 11.84GRR167 pKa = 11.84GIAFVQRR174 pKa = 11.84MHH176 pKa = 7.12DD177 pKa = 3.46QGGDD181 pKa = 3.56PAQGPQPQRR190 pKa = 11.84PWRR193 pKa = 11.84EE194 pKa = 3.55RR195 pKa = 11.84LRR197 pKa = 11.84DD198 pKa = 3.53RR199 pKa = 11.84AIQLYY204 pKa = 9.59EE205 pKa = 4.49AGTHH209 pKa = 6.5PIARR213 pKa = 11.84AWDD216 pKa = 3.42EE217 pKa = 4.12AQRR220 pKa = 11.84LMDD223 pKa = 4.15GPNYY227 pKa = 10.24RR228 pKa = 11.84ATLKK232 pKa = 10.6KK233 pKa = 10.11IAVTGMTACALFLAYY248 pKa = 10.19RR249 pKa = 11.84SWSGRR254 pKa = 11.84KK255 pKa = 8.9NGKK258 pKa = 8.89KK259 pKa = 10.05EE260 pKa = 3.78RR261 pKa = 11.84DD262 pKa = 3.89EE263 pKa = 5.13PEE265 pKa = 3.44MDD267 pKa = 4.04QIVPEE272 pKa = 4.93DD273 pKa = 3.83KK274 pKa = 10.69TPSPLMLRR282 pKa = 11.84EE283 pKa = 4.0VFMSGSTVLQNVPRR297 pKa = 11.84AVQGVRR303 pKa = 11.84LLRR306 pKa = 11.84LPEE309 pKa = 4.38NGSTRR314 pKa = 11.84QSYY317 pKa = 6.9MTCFKK322 pKa = 10.51VGRR325 pKa = 11.84FFVTCSHH332 pKa = 6.01VEE334 pKa = 3.93FGEE337 pKa = 4.41GEE339 pKa = 3.9EE340 pKa = 4.11AAIALEE346 pKa = 4.12AVPLDD351 pKa = 4.94SNPTWLRR358 pKa = 11.84ARR360 pKa = 11.84VLKK363 pKa = 9.85VFPRR367 pKa = 11.84RR368 pKa = 11.84TCEE371 pKa = 3.65LADD374 pKa = 4.91DD375 pKa = 4.26IVVLEE380 pKa = 5.16CPAQLATVRR389 pKa = 11.84SIPSRR394 pKa = 11.84VAKK397 pKa = 9.54EE398 pKa = 3.58NPIAGFVAGFVDD410 pKa = 5.38GVHH413 pKa = 7.07SIPAMSPGAIDD424 pKa = 3.21TEE426 pKa = 4.67GTHH429 pKa = 6.12WCTTAPGASGSAVCDD444 pKa = 3.52HH445 pKa = 7.14AGVAIGVHH453 pKa = 5.2YY454 pKa = 10.75AGSHH458 pKa = 5.78KK459 pKa = 10.66LNSFMPFDD467 pKa = 4.16RR468 pKa = 11.84EE469 pKa = 3.77VLDD472 pKa = 4.18FVKK475 pKa = 10.71GGNSEE480 pKa = 3.91PFEE483 pKa = 4.53RR484 pKa = 11.84SVKK487 pKa = 8.96RR488 pKa = 11.84TRR490 pKa = 11.84ASSQPNGGRR499 pKa = 11.84TPGLSPLQEE508 pKa = 4.03PTLEE512 pKa = 4.15ISDD515 pKa = 3.92TSGLDD520 pKa = 3.36PLPNLSGPKK529 pKa = 9.62SSSSTATSPPTDD541 pKa = 3.46AAPISRR547 pKa = 11.84SRR549 pKa = 11.84RR550 pKa = 11.84RR551 pKa = 11.84RR552 pKa = 11.84LARR555 pKa = 11.84RR556 pKa = 11.84LKK558 pKa = 10.44KK559 pKa = 10.9LSDD562 pKa = 3.51TCSEE566 pKa = 4.21SSANAGG572 pKa = 3.34

Molecular weight: 62.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1370

389

572

456.7

50.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.781 ± 0.216

1.533 ± 0.316

4.745 ± 0.63

5.401 ± 0.839

3.723 ± 0.41

8.321 ± 0.408

1.898 ± 0.521

3.65 ± 0.221

3.796 ± 0.342

8.978 ± 0.537

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.117 ± 0.3

2.774 ± 0.298

6.423 ± 0.668

3.869 ± 0.791

7.445 ± 0.551

7.518 ± 0.749

6.35 ± 0.535

7.007 ± 0.952

2.628 ± 0.519

2.044 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski