Ixeridium yellow mottle virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Calvusvirinae; Umbravirus

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U8YLV2|A0A1U8YLV2_9TOMB Putative movement protein OS=Ixeridium yellow mottle virus 2 OX=1817526 GN=orf4 PE=4 SV=1
MM1 pKa = 7.23VRR3 pKa = 11.84FADD6 pKa = 4.21EE7 pKa = 3.71EE8 pKa = 4.25WALLVCQQRR17 pKa = 11.84MTDD20 pKa = 3.1AHH22 pKa = 5.92QATLNEE28 pKa = 4.42WYY30 pKa = 9.59EE31 pKa = 4.14GTIEE35 pKa = 4.13TDD37 pKa = 3.22VYY39 pKa = 10.83IPLSGEE45 pKa = 3.72PTNACEE51 pKa = 4.33ALPVAEE57 pKa = 4.34PQVGPVAIPLDD68 pKa = 4.02GSHH71 pKa = 7.13RR72 pKa = 11.84STVDD76 pKa = 3.01ALAGSEE82 pKa = 4.13DD83 pKa = 3.84APGVSGDD90 pKa = 3.73ASSTTSLEE98 pKa = 4.37TYY100 pKa = 10.32LGAAHH105 pKa = 7.06EE106 pKa = 4.14LLKK109 pKa = 10.57IEE111 pKa = 4.66AEE113 pKa = 4.1IAGLQLPQTGEE124 pKa = 3.85EE125 pKa = 4.11EE126 pKa = 4.77AYY128 pKa = 10.38GPHH131 pKa = 6.28YY132 pKa = 10.24PSGYY136 pKa = 9.91CNNPCRR142 pKa = 11.84ALVPWQPLVIKK153 pKa = 9.95VPRR156 pKa = 11.84APAPVIYY163 pKa = 10.27CGYY166 pKa = 7.6TPGWEE171 pKa = 4.19EE172 pKa = 3.54SRR174 pKa = 11.84SPVEE178 pKa = 3.73RR179 pKa = 11.84LGAAVVEE186 pKa = 4.58TGNLLAGYY194 pKa = 9.44LFTKK198 pKa = 9.31VIPFAAGTVFHH209 pKa = 7.14SMAQLATMVAEE220 pKa = 5.06AEE222 pKa = 4.48QPCDD226 pKa = 3.5PTIEE230 pKa = 4.73GPTLPLEE237 pKa = 4.34EE238 pKa = 4.87RR239 pKa = 11.84GAAMGYY245 pKa = 8.72MNSAAIAMEE254 pKa = 3.86LRR256 pKa = 11.84RR257 pKa = 11.84RR258 pKa = 11.84FGRR261 pKa = 11.84PQATPANVEE270 pKa = 3.69LGGRR274 pKa = 11.84VARR277 pKa = 11.84EE278 pKa = 3.73ILSDD282 pKa = 3.55RR283 pKa = 11.84CRR285 pKa = 11.84ATRR288 pKa = 11.84SEE290 pKa = 3.58AWYY293 pKa = 8.87TSVEE297 pKa = 4.04ATKK300 pKa = 9.36MWLTPTLVDD309 pKa = 3.58MVHH312 pKa = 5.89ATGNVGFCC320 pKa = 4.19

Molecular weight: 34.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U8YMV9|A0A1U8YMV9_9TOMB RNA-directed RNA polymerase OS=Ixeridium yellow mottle virus 2 OX=1817526 GN=IYMV_gp1 PE=4 SV=1
MM1 pKa = 7.26TNVFNVYY8 pKa = 9.45TPDD11 pKa = 3.17TSKK14 pKa = 10.7QPRR17 pKa = 11.84GASANAGRR25 pKa = 11.84RR26 pKa = 11.84PPTGVGRR33 pKa = 11.84LWDD36 pKa = 3.8GHH38 pKa = 5.19NHH40 pKa = 5.63PGARR44 pKa = 11.84KK45 pKa = 9.19RR46 pKa = 11.84EE47 pKa = 4.5SNVRR51 pKa = 11.84TPPAPKK57 pKa = 10.23GPQDD61 pKa = 3.44HH62 pKa = 7.14AGQATHH68 pKa = 7.07AKK70 pKa = 9.79GNPKK74 pKa = 9.67HH75 pKa = 6.66RR76 pKa = 11.84GPVVHH81 pKa = 6.88PAAGGGVRR89 pKa = 11.84PTRR92 pKa = 11.84PRR94 pKa = 11.84RR95 pKa = 11.84LPRR98 pKa = 11.84DD99 pKa = 3.75GEE101 pKa = 4.28PLDD104 pKa = 4.51HH105 pKa = 7.34RR106 pKa = 11.84SNPAQRR112 pKa = 11.84KK113 pKa = 8.0RR114 pKa = 11.84RR115 pKa = 11.84VRR117 pKa = 11.84KK118 pKa = 9.85SKK120 pKa = 9.13LTPEE124 pKa = 3.47QGAAIRR130 pKa = 11.84ALSATLFDD138 pKa = 3.82TLIGYY143 pKa = 7.26GVRR146 pKa = 11.84KK147 pKa = 9.38GALLQHH153 pKa = 6.73CLRR156 pKa = 11.84AVRR159 pKa = 11.84DD160 pKa = 3.71SHH162 pKa = 6.85SEE164 pKa = 3.25WRR166 pKa = 11.84KK167 pKa = 9.13RR168 pKa = 11.84LQSVSPVGARR178 pKa = 11.84GEE180 pKa = 4.14PSGSQLPSSTADD192 pKa = 3.11CKK194 pKa = 10.43TSPEE198 pKa = 4.29AGTIGPIEE206 pKa = 4.35KK207 pKa = 9.45PAAVEE212 pKa = 4.24GTSAAGDD219 pKa = 3.94DD220 pKa = 3.9QPTLCRR226 pKa = 11.84SCTGHH231 pKa = 6.15NNVYY235 pKa = 10.78

Molecular weight: 25.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1677

235

872

419.3

45.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.72 ± 0.925

2.326 ± 0.262

3.936 ± 0.22

6.261 ± 1.066

2.922 ± 0.698

7.99 ± 0.663

2.504 ± 0.355

4.055 ± 0.584

3.578 ± 0.775

8.766 ± 0.962

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.509

3.518 ± 0.29

7.692 ± 0.785

3.637 ± 0.496

6.857 ± 0.933

5.307 ± 0.443

6.738 ± 0.219

7.275 ± 0.288

1.491 ± 0.224

2.803 ± 0.536
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski