Anoxybacter fermentans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiales incertae sedis; Anoxybacter

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3011 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9HRZ4|A0A3Q9HRZ4_9FIRM Probable 2-phosphosulfolactate phosphatase OS=Anoxybacter fermentans OX=1323375 GN=comB PE=3 SV=1
MM1 pKa = 7.55KK2 pKa = 10.51KK3 pKa = 10.43LLTLLTILILTVGLVGCDD21 pKa = 3.68FLPFMNKK28 pKa = 9.9APVIYY33 pKa = 10.44SLISEE38 pKa = 4.67KK39 pKa = 8.31TTIHH43 pKa = 6.54PGEE46 pKa = 4.04EE47 pKa = 3.63VKK49 pKa = 10.72IQVKK53 pKa = 10.5ASDD56 pKa = 4.11PNGDD60 pKa = 3.7SLVYY64 pKa = 9.04TWNATGGKK72 pKa = 9.57INGTGSEE79 pKa = 4.7VIWVAPQEE87 pKa = 4.05IGEE90 pKa = 4.06YY91 pKa = 9.22TITVKK96 pKa = 10.73VSDD99 pKa = 4.13SKK101 pKa = 11.77VDD103 pKa = 3.43VSKK106 pKa = 10.98SIQISVQPLPNEE118 pKa = 4.02APVISSLTVEE128 pKa = 4.35KK129 pKa = 10.86SVVQPEE135 pKa = 4.42EE136 pKa = 3.89KK137 pKa = 10.75VKK139 pKa = 11.03VQVQASDD146 pKa = 3.61PDD148 pKa = 4.39GDD150 pKa = 3.81VLTYY154 pKa = 9.15TWTVTGGSIEE164 pKa = 4.31GTGSEE169 pKa = 4.52VFWIASQTDD178 pKa = 3.44GNYY181 pKa = 9.57TITVEE186 pKa = 4.16VSDD189 pKa = 4.86GRR191 pKa = 11.84DD192 pKa = 3.09TVTGSINIQVNTAPIIEE209 pKa = 5.01EE210 pKa = 3.85ISASSSSVLVNGTLTLTAIVNDD232 pKa = 3.8PTGDD236 pKa = 3.31NLTYY240 pKa = 10.15RR241 pKa = 11.84WEE243 pKa = 4.13DD244 pKa = 3.41AIGEE248 pKa = 4.31VLGTEE253 pKa = 5.07PSLEE257 pKa = 3.6WHH259 pKa = 6.92APDD262 pKa = 3.5TYY264 pKa = 11.02GVYY267 pKa = 10.27PVTLYY272 pKa = 11.12VSDD275 pKa = 5.4GIFEE279 pKa = 4.14SSKK282 pKa = 9.61TININVEE289 pKa = 4.3DD290 pKa = 4.84DD291 pKa = 3.46SHH293 pKa = 6.5HH294 pKa = 6.41APEE297 pKa = 4.21IQEE300 pKa = 4.18VAITTPYY307 pKa = 10.45FEE309 pKa = 4.4SQVVVDD315 pKa = 4.53SVLAAGRR322 pKa = 11.84LYY324 pKa = 10.68AVWPIFVDD332 pKa = 3.44VDD334 pKa = 3.59EE335 pKa = 5.47SDD337 pKa = 3.53QLDD340 pKa = 3.63IQATADD346 pKa = 3.4VGEE349 pKa = 4.32VEE351 pKa = 4.66IFASEE356 pKa = 4.51YY357 pKa = 9.27EE358 pKa = 4.63TICFWVAPDD367 pKa = 3.4VAGEE371 pKa = 4.02YY372 pKa = 10.07TLTFTVTDD380 pKa = 4.77GIYY383 pKa = 9.78TVVKK387 pKa = 8.94EE388 pKa = 4.19YY389 pKa = 10.31PVQVVEE395 pKa = 4.32NSCPEE400 pKa = 3.31IDD402 pKa = 4.91AIYY405 pKa = 10.34INNEE409 pKa = 4.35LITDD413 pKa = 4.34NYY415 pKa = 8.63YY416 pKa = 10.15QAAPGEE422 pKa = 4.52EE423 pKa = 3.76ITISVLASDD432 pKa = 4.96SDD434 pKa = 3.6NDD436 pKa = 3.57APLEE440 pKa = 3.94YY441 pKa = 10.39KK442 pKa = 10.35IIDD445 pKa = 3.93PYY447 pKa = 10.62YY448 pKa = 10.04EE449 pKa = 3.42IWNTNEE455 pKa = 3.91LTFTAPMEE463 pKa = 4.16EE464 pKa = 4.34EE465 pKa = 3.86MDD467 pKa = 4.37EE468 pKa = 3.53IWIYY472 pKa = 11.28VFDD475 pKa = 4.21GLQVRR480 pKa = 11.84VASVIIDD487 pKa = 3.57VDD489 pKa = 3.48NGADD493 pKa = 4.44NNTNDD498 pKa = 5.13QNAIDD503 pKa = 4.35QNAIDD508 pKa = 4.57QINNLISRR516 pKa = 11.84FVNDD520 pKa = 5.01YY521 pKa = 10.62EE522 pKa = 4.27NQDD525 pKa = 3.25INSILSYY532 pKa = 10.6TDD534 pKa = 3.27SSYY537 pKa = 10.98QAEE540 pKa = 4.47LKK542 pKa = 10.68SRR544 pKa = 11.84LNRR547 pKa = 11.84YY548 pKa = 9.18FEE550 pKa = 4.51LEE552 pKa = 3.98RR553 pKa = 11.84VSNLTIRR560 pKa = 11.84KK561 pKa = 8.43LSEE564 pKa = 3.96IIEE567 pKa = 4.09VDD569 pKa = 3.47GEE571 pKa = 4.45FEE573 pKa = 4.19VQVAVQGDD581 pKa = 4.4FYY583 pKa = 11.72VLFEE587 pKa = 4.35TFPNSLIRR595 pKa = 11.84VFTFWFTYY603 pKa = 10.5DD604 pKa = 2.93EE605 pKa = 4.48YY606 pKa = 11.79GNILISSVWLL616 pKa = 3.62

Molecular weight: 68.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S9SVD0|A0A3S9SVD0_9FIRM Na_H_Exchanger domain-containing protein OS=Anoxybacter fermentans OX=1323375 GN=BBF96_01770 PE=4 SV=1
MM1 pKa = 7.25NLRR4 pKa = 11.84YY5 pKa = 9.49RR6 pKa = 11.84RR7 pKa = 11.84NKK9 pKa = 10.5LMTRR13 pKa = 11.84CLINFIAILITGHH26 pKa = 6.91LLDD29 pKa = 5.39GIVVNSLIAVLAAAFVLGIVNTFIRR54 pKa = 11.84PILVILSLPLTIITLGLFTFVINALMLLLTASLIPGFSISSFWTAFFGAIIISILSSIILAVFDD118 pKa = 4.0

Molecular weight: 12.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3011

0

3011

927558

37

3256

308.1

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.174 ± 0.05

0.915 ± 0.017

5.097 ± 0.037

7.741 ± 0.05

4.403 ± 0.039

7.027 ± 0.048

1.624 ± 0.018

9.115 ± 0.038

7.76 ± 0.049

10.255 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.022

4.527 ± 0.037

3.642 ± 0.028

3.196 ± 0.029

4.682 ± 0.036

4.986 ± 0.032

4.708 ± 0.036

6.845 ± 0.042

0.963 ± 0.016

3.911 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski