Arthrobacter sp. SW1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter; unclassified Arthrobacter

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3661 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1E8FNB8|A0A1E8FNB8_9MICC Transcriptional regulator OS=Arthrobacter sp. SW1 OX=1920889 GN=BIU82_10275 PE=4 SV=1
MM1 pKa = 7.2VFLAALSTLALSTFGLTGASADD23 pKa = 3.71PDD25 pKa = 4.01GQHH28 pKa = 6.87GSDD31 pKa = 3.56EE32 pKa = 4.14GHH34 pKa = 7.42LIGTGATGKK43 pKa = 9.57IQLLDD48 pKa = 3.71VVDD51 pKa = 4.11VTDD54 pKa = 4.19TPDD57 pKa = 4.39LIADD61 pKa = 3.8VTVSPDD67 pKa = 2.82GNTAFLANWGEE78 pKa = 4.02PDD80 pKa = 3.55CAGPEE85 pKa = 4.08TGGQNSPDD93 pKa = 3.18AGAYY97 pKa = 10.18VIDD100 pKa = 3.7ITNIEE105 pKa = 4.36TDD107 pKa = 3.6PEE109 pKa = 4.31SAKK112 pKa = 10.92LIGFIPSHH120 pKa = 6.27QDD122 pKa = 2.95TRR124 pKa = 11.84PGEE127 pKa = 4.14GMQVVNVTTAQFSGDD142 pKa = 3.2ILVMNNEE149 pKa = 4.18ACGKK153 pKa = 7.78NAKK156 pKa = 10.17GGVSLWDD163 pKa = 3.32VTDD166 pKa = 3.71PANPKK171 pKa = 9.96KK172 pKa = 10.6LSEE175 pKa = 4.26HH176 pKa = 6.22FGDD179 pKa = 4.36RR180 pKa = 11.84EE181 pKa = 4.03PGTRR185 pKa = 11.84DD186 pKa = 2.99ANEE189 pKa = 3.72IHH191 pKa = 7.12SAFAWDD197 pKa = 4.07AGNNAYY203 pKa = 10.72LVMTDD208 pKa = 3.75NEE210 pKa = 4.25QFPDD214 pKa = 3.64VDD216 pKa = 4.4ILDD219 pKa = 3.53ITNPHH224 pKa = 6.78RR225 pKa = 11.84PRR227 pKa = 11.84LIAEE231 pKa = 4.03YY232 pKa = 10.22NLNDD236 pKa = 3.83FAVDD240 pKa = 3.74QPEE243 pKa = 4.22LGLTEE248 pKa = 4.92SFLHH252 pKa = 6.65DD253 pKa = 3.67MVVKK257 pKa = 10.54EE258 pKa = 3.6IDD260 pKa = 3.19GRR262 pKa = 11.84FIMLLSYY269 pKa = 10.21WDD271 pKa = 3.57GGYY274 pKa = 10.86VLLDD278 pKa = 3.27VTDD281 pKa = 3.92PANAVFIGDD290 pKa = 3.62TDD292 pKa = 4.29FAAVDD297 pKa = 3.67PEE299 pKa = 4.37LFEE302 pKa = 4.53SLGVALTPEE311 pKa = 4.76GNGHH315 pKa = 5.0QAEE318 pKa = 4.36FTKK321 pKa = 11.06DD322 pKa = 2.91NAFFIGTDD330 pKa = 3.29EE331 pKa = 5.03DD332 pKa = 3.87FAPYY336 pKa = 8.79RR337 pKa = 11.84TDD339 pKa = 3.07ALRR342 pKa = 11.84ITSGAFQGEE351 pKa = 4.68YY352 pKa = 10.45EE353 pKa = 4.41SVIVPGGQPPAVLEE367 pKa = 4.4DD368 pKa = 3.84LTLSGPVVYY377 pKa = 10.22GGYY380 pKa = 10.05GCPASAPVPSPEE392 pKa = 4.37DD393 pKa = 3.33VPGYY397 pKa = 9.76VASLQPGDD405 pKa = 3.64EE406 pKa = 4.63TILVLQRR413 pKa = 11.84GPSGDD418 pKa = 3.53PSATEE423 pKa = 3.87EE424 pKa = 4.07ACFPGEE430 pKa = 4.17KK431 pKa = 9.73AHH433 pKa = 6.42EE434 pKa = 4.21AALAGWDD441 pKa = 3.03AVLFVQRR448 pKa = 11.84HH449 pKa = 4.77LPAGEE454 pKa = 4.41EE455 pKa = 3.97DD456 pKa = 3.73TAFCGSGAFVDD467 pKa = 5.16SIVAVCTTHH476 pKa = 6.86EE477 pKa = 4.26AFHH480 pKa = 6.76KK481 pKa = 10.56LFNLEE486 pKa = 4.38PLDD489 pKa = 4.12PAQWSYY495 pKa = 11.63PEE497 pKa = 4.13NLAIGAIGDD506 pKa = 4.43RR507 pKa = 11.84IRR509 pKa = 11.84VGSIFDD515 pKa = 3.07GWGYY519 pKa = 10.71VHH521 pKa = 7.34LFDD524 pKa = 4.95TATLQEE530 pKa = 4.75LDD532 pKa = 3.47TYY534 pKa = 10.83AIPEE538 pKa = 3.94AHH540 pKa = 7.03DD541 pKa = 3.2PAFAFGYY548 pKa = 10.52GDD550 pKa = 3.97LSVHH554 pKa = 5.78EE555 pKa = 4.57VAVDD559 pKa = 3.68PQDD562 pKa = 3.59PSLAYY567 pKa = 10.33LSYY570 pKa = 11.08YY571 pKa = 10.66SGGLRR576 pKa = 11.84AIQIQCGGVPYY587 pKa = 10.76DD588 pKa = 4.55ADD590 pKa = 3.93NPPADD595 pKa = 4.07KK596 pKa = 10.85GSCEE600 pKa = 3.93LVEE603 pKa = 4.28VGSYY607 pKa = 10.9LDD609 pKa = 3.49PAGNDD614 pKa = 3.07FWGVEE619 pKa = 4.16TFIGDD624 pKa = 3.83DD625 pKa = 3.15GRR627 pKa = 11.84TYY629 pKa = 11.0ILGSDD634 pKa = 3.72RR635 pKa = 11.84DD636 pKa = 3.64SGLWIFTDD644 pKa = 3.86PP645 pKa = 4.44

Molecular weight:
68.69 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1E8FVW3|A0A1E8FVW3_9MICC AB hydrolase-1 domain-containing protein OS=Arthrobacter sp. SW1 OX=1920889 GN=BIU82_02175 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.31VRR4 pKa = 11.84NSLRR8 pKa = 11.84ALKK11 pKa = 9.61KK12 pKa = 9.72IPGAQVVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GKK24 pKa = 8.59TFVINKK30 pKa = 8.84KK31 pKa = 9.15NPRR34 pKa = 11.84FKK36 pKa = 10.85ARR38 pKa = 11.84QGG40 pKa = 3.28

Molecular weight:
4.65 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3661

0

3661

1202471

37

2047

328.5

35.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.352 ± 0.059

0.625 ± 0.009

5.444 ± 0.036

5.87 ± 0.046

3.334 ± 0.03

9.295 ± 0.038

2.009 ± 0.019

4.279 ± 0.033

2.965 ± 0.033

10.381 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.978 ± 0.018

2.428 ± 0.024

5.438 ± 0.033

2.96 ± 0.022

6.409 ± 0.042

5.846 ± 0.025

5.55 ± 0.033

8.331 ± 0.037

1.415 ± 0.018

2.089 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski