bacterium HR25

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 982 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5YL89|A0A2H5YL89_9BACT Acetyltransf_6 domain-containing protein OS=bacterium HR25 OX=2035420 GN=HRbin25_00039 PE=4 SV=1
MM1 pKa = 7.62LSGPARR7 pKa = 11.84RR8 pKa = 11.84PWGLLLAGVLLAVLTALAFGSDD30 pKa = 3.77SPALPGLGGPRR41 pKa = 11.84LAQAIPPHH49 pKa = 6.6SPALDD54 pKa = 3.61TDD56 pKa = 4.96DD57 pKa = 6.18DD58 pKa = 5.41DD59 pKa = 7.91DD60 pKa = 6.85DD61 pKa = 5.49ILVKK65 pKa = 10.84DD66 pKa = 4.18RR67 pKa = 11.84DD68 pKa = 3.74RR69 pKa = 11.84DD70 pKa = 3.96GVPDD74 pKa = 3.72STDD77 pKa = 3.09NCPRR81 pKa = 11.84TANASQEE88 pKa = 4.18DD89 pKa = 4.17TDD91 pKa = 4.21GDD93 pKa = 4.14GRR95 pKa = 11.84GDD97 pKa = 3.52ACDD100 pKa = 4.39PDD102 pKa = 4.44LDD104 pKa = 4.24NDD106 pKa = 4.32GVPNTADD113 pKa = 3.56KK114 pKa = 10.99RR115 pKa = 11.84PVPAGPGVRR124 pKa = 11.84ALDD127 pKa = 3.66ATTTDD132 pKa = 3.02SDD134 pKa = 4.09GDD136 pKa = 4.24GVPDD140 pKa = 5.02ASDD143 pKa = 3.2NCPFVPNASQEE154 pKa = 4.09DD155 pKa = 4.09TDD157 pKa = 4.62RR158 pKa = 11.84DD159 pKa = 4.48GIGDD163 pKa = 3.83ACDD166 pKa = 3.46TDD168 pKa = 3.68GDD170 pKa = 4.16NDD172 pKa = 4.21GVPDD176 pKa = 4.49SSDD179 pKa = 3.38NCPQDD184 pKa = 3.77PNPDD188 pKa = 3.58QVDD191 pKa = 3.36SDD193 pKa = 4.13GDD195 pKa = 4.22GEE197 pKa = 5.34GDD199 pKa = 3.28ACDD202 pKa = 3.51PVAEE206 pKa = 4.2VFYY209 pKa = 11.28VRR211 pKa = 11.84STDD214 pKa = 3.2GGQTFGNYY222 pKa = 9.13YY223 pKa = 10.49NISNDD228 pKa = 3.35GLHH231 pKa = 6.67SGIAHH236 pKa = 6.05VAASGGVVHH245 pKa = 6.76IVWDD249 pKa = 3.98RR250 pKa = 11.84GDD252 pKa = 3.38EE253 pKa = 3.98PRR255 pKa = 11.84EE256 pKa = 3.71VVYY259 pKa = 10.57RR260 pKa = 11.84RR261 pKa = 11.84SLDD264 pKa = 3.45GGVSFAPIFNASSNNGDD281 pKa = 3.44SSEE284 pKa = 4.34SDD286 pKa = 3.3LTVGGSVLHH295 pKa = 5.89VVWEE299 pKa = 4.07EE300 pKa = 4.21DD301 pKa = 3.63VLDD304 pKa = 4.6ANGNPDD310 pKa = 3.73PCCPGDD316 pKa = 3.24EE317 pKa = 4.11AYY319 pKa = 10.67YY320 pKa = 10.39RR321 pKa = 11.84RR322 pKa = 11.84SGDD325 pKa = 3.13AGQTFSASRR334 pKa = 11.84NLSQTPNAHH343 pKa = 6.53SRR345 pKa = 11.84DD346 pKa = 3.71PDD348 pKa = 3.57VATSGNLVGVVHH360 pKa = 7.14EE361 pKa = 5.59DD362 pKa = 3.45PTPGNDD368 pKa = 3.69DD369 pKa = 3.38IFFEE373 pKa = 4.53RR374 pKa = 11.84STDD377 pKa = 4.25GGLTWSTFVNLSQDD391 pKa = 3.48AGQSAEE397 pKa = 3.97PAIDD401 pKa = 3.14ISGNTVHH408 pKa = 6.36VVWEE412 pKa = 4.29HH413 pKa = 5.84RR414 pKa = 11.84ASGRR418 pKa = 11.84IAYY421 pKa = 8.68VRR423 pKa = 11.84STDD426 pKa = 4.7GGVTWSAPLLLPAGADD442 pKa = 3.17RR443 pKa = 11.84AAIVAASGSTVHH455 pKa = 6.49VVACGGGEE463 pKa = 3.74VRR465 pKa = 11.84YY466 pKa = 10.09YY467 pKa = 10.97RR468 pKa = 11.84STDD471 pKa = 3.15GGATWGAPVNLSNNPGACSKK491 pKa = 10.95PMVDD495 pKa = 3.3VLGDD499 pKa = 3.23QVYY502 pKa = 8.83VVWEE506 pKa = 4.19DD507 pKa = 3.65TTTGASDD514 pKa = 3.53VYY516 pKa = 10.87LRR518 pKa = 11.84RR519 pKa = 11.84SSDD522 pKa = 2.99GGITFAPTQNLSNNAGEE539 pKa = 4.19SSEE542 pKa = 4.15PSVAVDD548 pKa = 3.5PSTGHH553 pKa = 6.53VYY555 pKa = 9.85VVWYY559 pKa = 10.0DD560 pKa = 3.89FTRR563 pKa = 11.84PTPPAAPADD572 pKa = 4.0ADD574 pKa = 3.84SDD576 pKa = 4.34GVADD580 pKa = 5.28PSDD583 pKa = 3.82NCPGVANPDD592 pKa = 3.32QADD595 pKa = 3.47ADD597 pKa = 4.14GDD599 pKa = 4.36GQGDD603 pKa = 3.73ACDD606 pKa = 4.69PDD608 pKa = 4.99DD609 pKa = 6.42DD610 pKa = 5.06NDD612 pKa = 4.21GVPDD616 pKa = 4.56ASDD619 pKa = 3.46NCPFAANAAQTDD631 pKa = 3.73TDD633 pKa = 4.04GDD635 pKa = 4.34GQGDD639 pKa = 3.91ACDD642 pKa = 4.48SDD644 pKa = 5.23DD645 pKa = 5.93DD646 pKa = 5.14GDD648 pKa = 4.27GVADD652 pKa = 4.56PFDD655 pKa = 4.14NCPLVPNAGQEE666 pKa = 4.12NTDD669 pKa = 3.35GDD671 pKa = 4.51GQGDD675 pKa = 3.93ACDD678 pKa = 5.58DD679 pKa = 4.59DD680 pKa = 6.45DD681 pKa = 7.02DD682 pKa = 6.22NDD684 pKa = 5.29GIPDD688 pKa = 3.9ASDD691 pKa = 3.66PCPLQSDD698 pKa = 4.32CDD700 pKa = 4.05SDD702 pKa = 4.48GVPDD706 pKa = 4.17SSDD709 pKa = 3.19NCRR712 pKa = 11.84LAFNPAQQDD721 pKa = 3.16SDD723 pKa = 3.84GDD725 pKa = 4.23GTGDD729 pKa = 4.36ACDD732 pKa = 3.6TDD734 pKa = 4.61GNPMLAFVAAGPGGATLYY752 pKa = 10.6EE753 pKa = 4.76FGRR756 pKa = 11.84GTTGEE761 pKa = 3.3IWYY764 pKa = 9.21RR765 pKa = 11.84AWDD768 pKa = 3.78GTAWSAWTGLSAVANSPPTVATSGADD794 pKa = 3.05LYY796 pKa = 11.63VFARR800 pKa = 11.84GSDD803 pKa = 3.28DD804 pKa = 3.5GLWYY808 pKa = 10.35RR809 pKa = 11.84RR810 pKa = 11.84LSGGTWDD817 pKa = 3.09AWTPLGGTMRR827 pKa = 11.84GVPAAVATAGGHH839 pKa = 5.4IYY841 pKa = 10.8VFVRR845 pKa = 11.84GASGDD850 pKa = 2.37IWYY853 pKa = 9.52RR854 pKa = 11.84HH855 pKa = 5.22WDD857 pKa = 3.42GTAWQPWASLGGLATEE873 pKa = 4.72SPGALALDD881 pKa = 3.71SEE883 pKa = 4.94VFLFVRR889 pKa = 11.84GSDD892 pKa = 3.23DD893 pKa = 3.49GLWYY897 pKa = 10.33RR898 pKa = 11.84RR899 pKa = 11.84LSGATWGDD907 pKa = 3.42WASLGGPVSSGPRR920 pKa = 11.84PVALGSVEE928 pKa = 4.15ADD930 pKa = 3.16VFARR934 pKa = 11.84GTDD937 pKa = 3.17NAVWYY942 pKa = 8.67RR943 pKa = 11.84HH944 pKa = 5.27WDD946 pKa = 3.43GTTWGAWQTLGGSLHH961 pKa = 6.79SPPQPLAVGGSLFLMALGTGDD982 pKa = 4.23DD983 pKa = 3.17LWYY986 pKa = 10.16RR987 pKa = 11.84QLSGGTWADD996 pKa = 3.32WQPLEE1001 pKa = 4.45GRR1003 pKa = 11.84LTAAPALVAFQSGAFVFGRR1022 pKa = 11.84GSEE1025 pKa = 3.97GDD1027 pKa = 2.84VWYY1030 pKa = 10.41RR1031 pKa = 11.84HH1032 pKa = 5.49WDD1034 pKa = 3.41GTTWGAWQPLGGLVTAGVPP1053 pKa = 3.42

Molecular weight: 109.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5YMZ8|A0A2H5YMZ8_9BACT Acetolactate synthase large subunit IlvG OS=bacterium HR25 OX=2035420 GN=ilvG_2 PE=3 SV=1
MM1 pKa = 8.03PKK3 pKa = 8.99RR4 pKa = 11.84TYY6 pKa = 9.41QPKK9 pKa = 8.73NRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84QRR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 5.04GFLARR22 pKa = 11.84NRR24 pKa = 11.84TRR26 pKa = 11.84SGRR29 pKa = 11.84AVLKK33 pKa = 10.34RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84LKK38 pKa = 10.82GRR40 pKa = 11.84WRR42 pKa = 11.84LAVV45 pKa = 3.35

Molecular weight: 5.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

982

0

982

285183

30

1256

290.4

31.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.366 ± 0.098

0.935 ± 0.026

4.508 ± 0.059

7.503 ± 0.082

3.046 ± 0.047

9.062 ± 0.08

2.064 ± 0.039

3.865 ± 0.067

1.83 ± 0.046

11.839 ± 0.101

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.005 ± 0.032

1.729 ± 0.036

6.154 ± 0.066

3.624 ± 0.054

9.238 ± 0.084

4.399 ± 0.05

4.317 ± 0.049

8.456 ± 0.063

1.482 ± 0.04

2.578 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski