Candidatus Marinamargulisbacteria bacterium SCGC AAA071-K20

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Candidatus Margulisbacteria; Candidatus Marinamargulisbacteria

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1682 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328RUG5|A0A328RUG5_9BACT Metal-dependent hydrolase OS=Candidatus Marinamargulisbacteria bacterium SCGC AAA071-K20 OX=2184347 GN=DID80_01320 PE=4 SV=1
MM1 pKa = 7.44ISYY4 pKa = 8.06VTEE7 pKa = 4.02PTGEE11 pKa = 3.92WVSYY15 pKa = 11.33DD16 pKa = 3.23DD17 pKa = 3.89TNSIRR22 pKa = 11.84EE23 pKa = 4.15KK24 pKa = 10.84VNYY27 pKa = 9.79IQDD30 pKa = 3.77QNLGGAMIWEE40 pKa = 4.17LTGDD44 pKa = 4.18DD45 pKa = 4.34EE46 pKa = 5.4NHH48 pKa = 6.49TLLQLIHH55 pKa = 6.77SEE57 pKa = 4.35LNDD60 pKa = 3.38

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328RWP4|A0A328RWP4_9BACT Alanine--tRNA ligase OS=Candidatus Marinamargulisbacteria bacterium SCGC AAA071-K20 OX=2184347 GN=alaS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 10.37RR3 pKa = 11.84PFQPNTRR10 pKa = 11.84KK11 pKa = 9.92RR12 pKa = 11.84NKK14 pKa = 9.41KK15 pKa = 9.38HH16 pKa = 5.44GFLVRR21 pKa = 11.84MRR23 pKa = 11.84TVGGRR28 pKa = 11.84RR29 pKa = 11.84VIRR32 pKa = 11.84SRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84HH40 pKa = 4.25QLAAA44 pKa = 4.03

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1682

0

1682

524630

28

8757

311.9

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.105 ± 0.105

0.982 ± 0.024

5.549 ± 0.05

6.199 ± 0.061

4.923 ± 0.048

6.123 ± 0.072

1.992 ± 0.025

7.869 ± 0.063

8.075 ± 0.074

10.211 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.116 ± 0.028

5.187 ± 0.045

3.612 ± 0.031

3.478 ± 0.04

3.697 ± 0.038

7.648 ± 0.063

5.899 ± 0.052

6.267 ± 0.047

0.769 ± 0.018

3.299 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski