Lactococcus phage ul36.k1t1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5GYQ9|A5GYQ9_9CAUD Uncharacterized protein OS=Lactococcus phage ul36.k1t1 OX=374526 PE=4 SV=1
MM1 pKa = 7.08SVKK4 pKa = 9.84VHH6 pKa = 6.15FSNGEE11 pKa = 4.04SIVISEE17 pKa = 4.5EE18 pKa = 3.94TRR20 pKa = 11.84ISAWNSLDD28 pKa = 3.64KK29 pKa = 11.49DD30 pKa = 3.32PDD32 pKa = 3.46GYY34 pKa = 10.6YY35 pKa = 10.77AEE37 pKa = 5.18GVFSGSNIDD46 pKa = 4.29SPDD49 pKa = 3.38LGTSYY54 pKa = 10.29QHH56 pKa = 6.52IGLMGLFGSTDD67 pKa = 2.74WFAIGLDD74 pKa = 3.72FKK76 pKa = 9.31TTYY79 pKa = 8.25KK80 pKa = 10.22TSAIVSLEE88 pKa = 3.88EE89 pKa = 4.31TPWW92 pKa = 4.61

Molecular weight: 10.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5GYL3|A5GYL3_9CAUD Putative holin OS=Lactococcus phage ul36.k1t1 OX=374526 PE=4 SV=1
MM1 pKa = 7.71EE2 pKa = 4.14EE3 pKa = 3.94QAWRR7 pKa = 11.84EE8 pKa = 3.86VLEE11 pKa = 3.96RR12 pKa = 11.84LARR15 pKa = 11.84IEE17 pKa = 4.27TKK19 pKa = 10.35LDD21 pKa = 3.19NYY23 pKa = 9.17EE24 pKa = 4.14TVRR27 pKa = 11.84DD28 pKa = 3.65KK29 pKa = 11.59AEE31 pKa = 3.94RR32 pKa = 11.84ALLIAQSNAKK42 pKa = 9.99HH43 pKa = 5.57IEE45 pKa = 3.94KK46 pKa = 9.87MEE48 pKa = 4.71ANNKK52 pKa = 6.92WAWGFMLTLAVTVIGYY68 pKa = 9.08LFTKK72 pKa = 9.54IRR74 pKa = 11.84FRR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84VKK80 pKa = 10.61

Molecular weight: 9.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

10993

52

1027

207.4

23.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.677 ± 0.442

0.5 ± 0.136

6.195 ± 0.245

7.377 ± 0.696

4.303 ± 0.216

6.522 ± 0.463

1.274 ± 0.178

7.323 ± 0.47

8.615 ± 0.578

7.705 ± 0.36

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.329 ± 0.225

5.776 ± 0.261

3.184 ± 0.157

4.121 ± 0.22

3.411 ± 0.284

6.986 ± 0.334

6.65 ± 0.52

6.049 ± 0.257

1.319 ± 0.136

3.684 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski