Human picobirnavirus (strain Human/Thailand/Hy005102/-) (PBV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Picobirnaviridae; Picobirnavirus; Human picobirnavirus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q50LE6|ORF1_HPBVH Uncharacterized ORF1 protein OS=Human picobirnavirus (strain Human/Thailand/Hy005102/-) OX=647332 GN=Segment-1 PE=4 SV=1
MM1 pKa = 7.14KK2 pKa = 10.7QNDD5 pKa = 4.09TKK7 pKa = 10.72KK8 pKa = 6.93TTQRR12 pKa = 11.84RR13 pKa = 11.84NSKK16 pKa = 10.14KK17 pKa = 10.38YY18 pKa = 9.4SSKK21 pKa = 9.73TNRR24 pKa = 11.84GTKK27 pKa = 9.57RR28 pKa = 11.84APRR31 pKa = 11.84DD32 pKa = 3.52QEE34 pKa = 4.18VGTGAQEE41 pKa = 3.97STRR44 pKa = 11.84NDD46 pKa = 3.4VAWYY50 pKa = 10.23ARR52 pKa = 11.84YY53 pKa = 8.65PHH55 pKa = 7.38ILEE58 pKa = 4.27EE59 pKa = 4.17ATRR62 pKa = 11.84LPFAYY67 pKa = 9.51PIGQYY72 pKa = 10.67YY73 pKa = 7.98DD74 pKa = 2.97TGYY77 pKa = 10.75SVASATEE84 pKa = 3.58WSKK87 pKa = 11.59YY88 pKa = 9.77VDD90 pKa = 3.43TSLTIPGVMCVNFTPTPGEE109 pKa = 4.32SYY111 pKa = 11.1NKK113 pKa = 10.04NSPINIAAQNVYY125 pKa = 10.08TYY127 pKa = 10.27VRR129 pKa = 11.84HH130 pKa = 5.77MNSGHH135 pKa = 6.72ANYY138 pKa = 9.57EE139 pKa = 4.01QADD142 pKa = 3.63LMMYY146 pKa = 10.3LLAMDD151 pKa = 4.35SLYY154 pKa = 10.49IFHH157 pKa = 7.1SYY159 pKa = 7.64VRR161 pKa = 11.84KK162 pKa = 9.72ILAISKK168 pKa = 10.38LYY170 pKa = 10.45TPVNKK175 pKa = 10.07YY176 pKa = 9.55FPRR179 pKa = 11.84ALLVALGVDD188 pKa = 3.84PEE190 pKa = 4.92DD191 pKa = 3.58VFANQAQWEE200 pKa = 4.65YY201 pKa = 9.74FVNMVAYY208 pKa = 10.26RR209 pKa = 11.84AGAFAAPASMTYY221 pKa = 8.88YY222 pKa = 10.18EE223 pKa = 3.98RR224 pKa = 11.84HH225 pKa = 4.89AWMSNGLYY233 pKa = 10.02VDD235 pKa = 3.52QDD237 pKa = 3.46VTRR240 pKa = 11.84AQIYY244 pKa = 6.99MFKK247 pKa = 9.65PTMLWKK253 pKa = 10.67YY254 pKa = 10.02EE255 pKa = 4.01NLGTTGTKK263 pKa = 9.96LVPLMMPKK271 pKa = 10.51AGDD274 pKa = 3.34NRR276 pKa = 11.84KK277 pKa = 9.33LVDD280 pKa = 4.11FQVLFNNLVSTMLGDD295 pKa = 3.57EE296 pKa = 5.0DD297 pKa = 4.39FGIMSGDD304 pKa = 3.37VFKK307 pKa = 11.38AFGADD312 pKa = 3.34GLVKK316 pKa = 10.53LLAVDD321 pKa = 4.02STTMTLPTYY330 pKa = 10.66DD331 pKa = 4.53PLILAQIHH339 pKa = 5.3SARR342 pKa = 11.84AVGAPILEE350 pKa = 4.42TSTLTGFPGRR360 pKa = 11.84QWQITQNPDD369 pKa = 2.85VNNGAIIFHH378 pKa = 7.01PSFGYY383 pKa = 10.41DD384 pKa = 3.4GQDD387 pKa = 3.14HH388 pKa = 6.95EE389 pKa = 4.76EE390 pKa = 3.86LSFRR394 pKa = 11.84AMCSNMILNLPGEE407 pKa = 4.19AHH409 pKa = 5.82SAEE412 pKa = 4.51MIIEE416 pKa = 4.25ATRR419 pKa = 11.84LATMFQVKK427 pKa = 9.63AVPAGDD433 pKa = 3.35TSKK436 pKa = 10.89PVLYY440 pKa = 10.23LPNGFGTEE448 pKa = 4.11VVNDD452 pKa = 3.48YY453 pKa = 11.01TMISVDD459 pKa = 3.15KK460 pKa = 9.82ATPHH464 pKa = 7.31DD465 pKa = 4.23LTIHH469 pKa = 5.79TFFNNILVPNAKK481 pKa = 9.74EE482 pKa = 3.85NYY484 pKa = 8.24VANLEE489 pKa = 4.14LLNNIIQFDD498 pKa = 4.25WAPQLYY504 pKa = 8.16LTYY507 pKa = 10.72GIAQEE512 pKa = 4.37SFGPFAQLNDD522 pKa = 3.39WTILTGEE529 pKa = 4.27TLARR533 pKa = 11.84MHH535 pKa = 6.16EE536 pKa = 4.32VCVTSMFDD544 pKa = 3.43VPQMGFNKK552 pKa = 10.34

Molecular weight: 62.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q50LE6|ORF1_HPBVH Uncharacterized ORF1 protein OS=Human picobirnavirus (strain Human/Thailand/Hy005102/-) OX=647332 GN=Segment-1 PE=4 SV=1
MM1 pKa = 7.23TANQIAYY8 pKa = 9.41QKK10 pKa = 9.95HH11 pKa = 6.14LEE13 pKa = 4.19TARR16 pKa = 11.84VNAVGEE22 pKa = 4.17MQRR25 pKa = 11.84GLEE28 pKa = 3.89LDD30 pKa = 3.21EE31 pKa = 4.5SRR33 pKa = 11.84RR34 pKa = 11.84HH35 pKa = 6.31NISQEE40 pKa = 3.84QLKK43 pKa = 8.74TRR45 pKa = 11.84EE46 pKa = 4.1LTEE49 pKa = 4.06LEE51 pKa = 4.02RR52 pKa = 11.84SNRR55 pKa = 11.84AVEE58 pKa = 4.37KK59 pKa = 8.64EE60 pKa = 4.25TSRR63 pKa = 11.84HH64 pKa = 4.11NVVTEE69 pKa = 3.92TEE71 pKa = 4.18TRR73 pKa = 11.84RR74 pKa = 11.84SNLARR79 pKa = 11.84EE80 pKa = 4.2WEE82 pKa = 4.58TYY84 pKa = 9.78RR85 pKa = 11.84SNSARR90 pKa = 11.84EE91 pKa = 4.02MEE93 pKa = 4.39TQRR96 pKa = 11.84SNISYY101 pKa = 9.5EE102 pKa = 4.08AIKK105 pKa = 10.39RR106 pKa = 11.84GQLALDD112 pKa = 3.36RR113 pKa = 11.84AEE115 pKa = 4.19LNEE118 pKa = 4.57SIRR121 pKa = 11.84ATNEE125 pKa = 3.33NLALQYY131 pKa = 11.24SKK133 pKa = 11.22LQTEE137 pKa = 4.27SLLTQRR143 pKa = 11.84GQDD146 pKa = 3.68LQHH149 pKa = 6.65KK150 pKa = 8.89NAIIGASANAFGSLLGYY167 pKa = 8.59STASADD173 pKa = 3.62RR174 pKa = 11.84ASRR177 pKa = 11.84EE178 pKa = 4.2EE179 pKa = 3.74IASANRR185 pKa = 11.84KK186 pKa = 7.17SQEE189 pKa = 4.54HH190 pKa = 5.82IASMQVLGSMANTMFSSVSNLVGKK214 pKa = 7.87TAGAFAGGLSS224 pKa = 3.43

Molecular weight: 24.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1310

224

552

436.7

49.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.015 ± 1.279

0.916 ± 0.385

5.267 ± 1.024

5.725 ± 1.457

3.588 ± 0.781

6.412 ± 0.446

2.061 ± 0.092

4.427 ± 0.172

4.962 ± 0.606

8.244 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.893 ± 0.378

5.267 ± 0.928

4.275 ± 1.246

4.504 ± 0.495

5.725 ± 1.366

6.87 ± 1.338

6.489 ± 0.914

6.87 ± 0.999

1.756 ± 0.464

4.733 ± 0.766
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski