Marinobacterium sp. AK27

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Marinobacterium; unclassified Marinobacterium

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4141 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A081G436|A0A081G436_9GAMM Biotin carboxylase OS=Marinobacterium sp. AK27 OX=1232683 GN=ADIMK_0498 PE=4 SV=1
MM1 pKa = 7.35NRR3 pKa = 11.84QLQGGMRR10 pKa = 11.84KK11 pKa = 7.82QQQGVVLVLVAIAMLALLAMAGLALDD37 pKa = 4.62GGHH40 pKa = 7.46LLLNKK45 pKa = 9.09TRR47 pKa = 11.84LQNAVDD53 pKa = 3.72AAALSAARR61 pKa = 11.84TIADD65 pKa = 3.92FPKK68 pKa = 9.05DD69 pKa = 3.46TAVGTVHH76 pKa = 6.56TAAEE80 pKa = 4.24ASALAAFLSNLGLDD94 pKa = 5.01DD95 pKa = 4.46SAEE98 pKa = 4.08LNDD101 pKa = 4.65AYY103 pKa = 10.6ASSGSPLQVQFSTTLNPFVDD123 pKa = 3.87SATALPYY130 pKa = 10.62VRR132 pKa = 11.84VIAQDD137 pKa = 3.5LQLDD141 pKa = 3.77VWFLQVVGMADD152 pKa = 3.45KK153 pKa = 10.24PVSASAVAGPIGLNANTCDD172 pKa = 3.52ILPILACGCDD182 pKa = 3.26TDD184 pKa = 4.72GTYY187 pKa = 10.26TPDD190 pKa = 5.01NPDD193 pKa = 4.38DD194 pKa = 4.55DD195 pKa = 6.14CSDD198 pKa = 3.43SNTYY202 pKa = 10.23FGYY205 pKa = 9.95PDD207 pKa = 3.75NDD209 pKa = 3.69NNDD212 pKa = 3.43VEE214 pKa = 4.65SLGDD218 pKa = 3.27ISVIKK223 pKa = 10.3LAGGSGSDD231 pKa = 3.25VGPGNFRR238 pKa = 11.84LLRR241 pKa = 11.84LDD243 pKa = 3.36GTGGDD248 pKa = 4.32DD249 pKa = 5.55LRR251 pKa = 11.84DD252 pKa = 3.56ALAGVGDD259 pKa = 3.87SCANFGDD266 pKa = 4.71DD267 pKa = 4.1SAADD271 pKa = 3.74TEE273 pKa = 4.73PGNKK277 pKa = 9.43SGPVGQGLNTRR288 pKa = 11.84LGIYY292 pKa = 9.2QGPVDD297 pKa = 4.65PTTAPADD304 pKa = 3.74EE305 pKa = 4.34VTYY308 pKa = 9.48TYY310 pKa = 11.37YY311 pKa = 10.83SGSTPAGPTPVGPNPLVLDD330 pKa = 4.17GLDD333 pKa = 3.65DD334 pKa = 3.7QGVPVIKK341 pKa = 10.34RR342 pKa = 11.84QDD344 pKa = 3.15GSTSIDD350 pKa = 3.29GLFDD354 pKa = 3.46YY355 pKa = 10.92QDD357 pKa = 3.65YY358 pKa = 10.99LDD360 pKa = 5.33EE361 pKa = 4.39YY362 pKa = 9.11TSNYY366 pKa = 9.18PDD368 pKa = 3.86CQGSGCRR375 pKa = 11.84RR376 pKa = 11.84RR377 pKa = 11.84EE378 pKa = 3.82LVLPIGDD385 pKa = 4.01CTGSISGTSEE395 pKa = 3.78DD396 pKa = 3.45VHH398 pKa = 8.0VYY400 pKa = 10.47SVGCFFLMQNVDD412 pKa = 3.27VGTDD416 pKa = 3.31AEE418 pKa = 4.59VFGQFFTEE426 pKa = 5.08SEE428 pKa = 4.19GCDD431 pKa = 3.32AIGTFTSTPTSDD443 pKa = 3.47PVPIKK448 pKa = 10.27IVLYY452 pKa = 10.16RR453 pKa = 11.84DD454 pKa = 3.47SDD456 pKa = 3.87SGDD459 pKa = 3.29SS460 pKa = 3.44

Molecular weight: 47.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A081FVI1|A0A081FVI1_9GAMM ATP synthase subunit alpha OS=Marinobacterium sp. AK27 OX=1232683 GN=atpA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84QVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.89SLTVV44 pKa = 3.12

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4141

0

4141

1346198

37

3254

325.1

35.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.954 ± 0.043

1.077 ± 0.014

5.638 ± 0.03

6.459 ± 0.034

3.649 ± 0.023

7.662 ± 0.034

2.248 ± 0.021

5.557 ± 0.028

3.583 ± 0.025

11.341 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.017

3.126 ± 0.019

4.527 ± 0.024

4.217 ± 0.027

6.303 ± 0.037

6.136 ± 0.027

4.923 ± 0.026

7.012 ± 0.031

1.294 ± 0.014

2.681 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski