Synechococcus phage S-SKS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Llyrvirus; Synechococcus virus SSKS1

Average proteome isoelectric point is 5.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 281 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4QS45|M4QS45_9CAUD Uncharacterized protein OS=Synechococcus phage S-SKS1 OX=754042 GN=SWZG_00202 PE=4 SV=1
MM1 pKa = 7.8NYY3 pKa = 8.73TLKK6 pKa = 10.71QLADD10 pKa = 3.93RR11 pKa = 11.84VNSMIKK17 pKa = 9.97EE18 pKa = 3.94QGEE21 pKa = 4.25DD22 pKa = 3.62AEE24 pKa = 4.52CAAWIYY30 pKa = 9.2TKK32 pKa = 10.79NDD34 pKa = 3.03CHH36 pKa = 8.18LKK38 pKa = 10.99DD39 pKa = 3.58EE40 pKa = 5.64DD41 pKa = 4.47GNTDD45 pKa = 3.15YY46 pKa = 11.63GNNVEE51 pKa = 5.17DD52 pKa = 3.92PALIARR58 pKa = 11.84IFDD61 pKa = 3.76DD62 pKa = 4.03VGQIDD67 pKa = 4.67YY68 pKa = 10.0IYY70 pKa = 11.08TMIQDD75 pKa = 3.96CVDD78 pKa = 3.6EE79 pKa = 4.45VVEE82 pKa = 4.24EE83 pKa = 4.03QLVQYY88 pKa = 7.77QQEE91 pKa = 4.32MVEE94 pKa = 4.57TKK96 pKa = 10.61

Molecular weight: 11.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4QPR3|M4QPR3_9CAUD Uncharacterized protein OS=Synechococcus phage S-SKS1 OX=754042 GN=SWZG_00266 PE=4 SV=1
MM1 pKa = 7.45AVGAIAAGALRR12 pKa = 11.84IGSMFAKK19 pKa = 10.45GAGSVLSGGARR30 pKa = 11.84GVGRR34 pKa = 11.84SGGMLRR40 pKa = 11.84RR41 pKa = 11.84AVLKK45 pKa = 9.79KK46 pKa = 10.79SKK48 pKa = 10.45VKK50 pKa = 10.53RR51 pKa = 11.84EE52 pKa = 3.74NIARR56 pKa = 11.84SKK58 pKa = 11.08SFNKK62 pKa = 10.37KK63 pKa = 9.78LMEE66 pKa = 4.52RR67 pKa = 11.84NKK69 pKa = 10.14RR70 pKa = 11.84RR71 pKa = 11.84QKK73 pKa = 10.95EE74 pKa = 4.06KK75 pKa = 10.98LL76 pKa = 3.39

Molecular weight: 8.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

281

0

281

56029

49

1901

199.4

22.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.626 ± 0.178

1.173 ± 0.09

6.581 ± 0.115

6.709 ± 0.285

4.292 ± 0.122

7.446 ± 0.28

1.594 ± 0.098

7.061 ± 0.171

6.274 ± 0.296

7.419 ± 0.134

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.304 ± 0.134

6.184 ± 0.18

3.634 ± 0.133

3.211 ± 0.09

3.887 ± 0.132

7.637 ± 0.193

7.087 ± 0.406

6.27 ± 0.131

1.203 ± 0.073

4.408 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski